Restriction map for
pF1KB9553
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI AhdI AlwI
AocI ApaI ApoI AscI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FspI
HgaI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 749 GACGTC GACGT^C
AccI 696 GTCTAC GT^[AC][GT]AC
AcyI 749 GACGTC G[GA]^CG[CT]C
Alw44I 509 GTGCAC G^TGCAC
Aor51HI 404 AGCGCT AGC^GCT
ApaLI 509 GTGCAC G^TGCAC
AseI 774 ATTAAT AT^TAAT
AsnI 774 ATTAAT AT^TAAT
AspI 633 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 205 CCCGAG C^[CT]CG[GA]G
BanI 117 GGTGCC G^G[CT][GA]CC
BbvCI 219 GCTGAGG CCTCAGC(-5/-2)
BfaI 340 CTAG C^TAG
BglI 94 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 506 TACGTG [CT]AC^GT[GA]
BsaHI 749 GACGTC G[GA]^CG[CT]C
BsgI 526 GTGCAG GTGCAG(16/14)
BsmI 616 GCATTC GAATGC(1/-1)
BsoBI 205 CCCGAG C^[CT]CG[GA]G
BspMI 522 GCAGGT ACCTGC(4/8)
BsrFI 94 GCCGGC [GA]^CCGG[CT]
BssHII 172 GCGCGC G^CGCGC
BstAPI 519 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 94 GCCGGC [GA]^CCGG[CT]
DpnII 265 GATC ^GATC
DsaI 562 CCATGG C^C[GA][CT]GG
Eco47III 404 AGCGCT AGC^GCT
EcoRV 603 GATATC GAT^ATC
FseI 93 GGCCGGCC GGCCGG^CC
Hin1I 749 GACGTC G[GA]^CG[CT]C
HphI 541 TCACC GGTGA(8/7)
MaeI 340 CTAG C^TAG
MaeIII 540 GTCAC ^GT[ACGT]AC
MboI 265 GATC ^GATC
NaeI 94 GCCGGC GCC^GGC
NcoI 562 CCATGG C^CATGG
NdeII 265 GATC ^GATC
NgoMIV 94 GCCGGC G^CCGGC
PflFI 633 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 774 ATTAAT AT^TAAT
PstI 517 CTGCAG CTGCA^G
SacI 684 GAGCTC GAGCT^C
Sau3AI 265 GATC ^GATC
SfaNI 418 GATGC GCATC(5/9)
SfcI 517 CTGCAG C^T[GA][CT]AG
SfiI 93 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I 540 GTCAC ^GT[GC]AC
Tth111I 633 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 18 CGCG CG^CG
173 CGCG
AspHI 509 GTGCAC G[AT]GC[AT]^C
684 GAGCTC
BpmI 547 CTCCAG CTGGAG(16/14)
678 CTGGAG
BsiEI 29 CGGCCG CG[GA][CT]^CG
92 CGGCCG
BsiHKAI 509 GTGCAC G[AT]GC[AT]^C
684 GAGCTC
BsmFI 37 GGGAC GGGAC(10/14)
747 GGGAC
BsrBI 109 CCGCTC CCGCTC(-3/-3)
153 GAGCGG
BstF5I 413 GGATG GGATG(2/0)
417 GGATG
BstUI 18 CGCG CG^CG
173 CGCG
EagI 29 CGGCCG C^GGCCG
92 CGGCCG
EclXI 29 CGGCCG C^GGCCG
92 CGGCCG
Eco52I 29 CGGCCG C^GGCCG
92 CGGCCG
EcoT14I 449 CCAAGG C^C[AT][AT]GG
562 CCATGG
FauI 70 CCCGC CCCGC(4/6)
155 GCGGG
FokI 413 GGATG GGATG(9/13)
417 GGATG
MaeII 507 ACGT A^CGT
750 ACGT
MseI 397 TTAA T^TAA
775 TTAA
MvnI 18 CGCG CG^CG
173 CGCG
PpuMI 37 GGGACCC [GA]G^G[AT]CC[CT]
707 AGGACCC
StyI 449 CCAAGG C^C[AT][AT]GG
562 CCATGG
TaqI 267 TCGA T^CGA
279 TCGA
TfiI 567 GATTC G^A[AT]TC
591 GATTC
Tru9I 397 TTAA T^TAA
775 TTAA
TspRI 470 CAGTG CA[GC]TG[ACGT][ACGT]^
742 CAGTG
TthHB8I 267 TCGA T^CGA
279 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MboII MnlI MspI MvaI MwoI
NciI NlaIII NlaIV PleI Sau96I
ScrFI TseI