Restriction map for
pF1KB9551
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII AccI AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BclI
BfrI BglII BlnI BsaAI BsaI
BseRI BsgI BsiEI BsiWI BsmI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DraI DraII
DraIII EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KspI MfeI MluI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 393 GGTACC G^GTACC
AccIII 1019 TCCGGA T^CCGGA
AflIII 845 ACATGT A^C[GA][CT]GT
AocI 357 CCTCAGG CC^T[ACGT]AGG
Asp718I 393 GGTACC G^GTACC
AspHI 891 GTGCTC G[AT]GC[AT]^C
AvaI 547 CCCGAG C^[CT]CG[GA]G
BanII 343 GAGCCC G[GA]GC[CT]^C
BbsI 969 GAAGAC GAAGAC(2/6)
BbvI 597 GCTGC GCAGC(8/12)
BcgI 20 GCAAGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 238 GGATAC GTATCC(6/5)
BglI 1135 GCCAACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 969 GAAGAC GAAGAC(2/6)
BsaBI 1011 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1019 TCCGGA [AT]^CCGG[AT]
BseAI 1019 TCCGGA T^CCGGA
BsiHKAI 891 GTGCTC G[AT]GC[AT]^C
BsmBI 602 GAGACG CGTCTC(1/5)
BsoBI 547 CCCGAG C^[CT]CG[GA]G
Bsp1407I 128 TGTACA T^GTACA
BspDI 583 ATCGAT AT^CGAT
BspEI 1019 TCCGGA T^CCGGA
BspLU11I 845 ACATGT A^CATGT
BsrGI 128 TGTACA T^GTACA
BssSI 1113 CACGAG CACGAG(-5/-1)
BstAPI 591 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 563 CCATCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1091 AGATCC [GA]^GATC[CT]
Bsu36I 357 CCTCAGG CC^T[ACGT]AGG
ClaI 583 ATCGAT AT^CGAT
DrdI 775 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 746 CCACGG C^C[GA][CT]GG
Eco81I 357 CCTCAGG CC^T[ACGT]AGG
EcoNI 353 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 597 GCTGC GC^[ACGT]GC
HaeII 12 GGCGCT [GA]GCGC^[CT]
ItaI 597 GCTGC GC^[ACGT]GC
KpnI 393 GGTACC GGTAC^C
MaeII 694 ACGT A^CGT
MamI 1011 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1091 AGATCC [GA]^GATC[CT]
MroI 1019 TCCGGA T^CCGGA
MseI 271 TTAA T^TAA
NspI 845 ACATGT [GA]CATG^[CT]
SspBI 128 TGTACA T^GTACA
TfiI 294 GATTC G^A[AT]TC
Tru9I 271 TTAA T^TAA
TseI 597 GCTGC G^C[AT]GC
XhoII 1091 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 781 CGCG CG^CG
1096 CGCG
AlwNI 592 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1070 CAGACGCTG
Asp700I 289 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
812 GAAACATTTC
BanI 393 GGTACC G^G[CT][GA]CC
1046 GGTGCC
BcnI 945 CCGGG CC^[GC]GG
1146 CCCGG
BfaI 160 CTAG C^TAG
250 CTAG
BlpI 15 GCTGAGC GC^T[ACGT]AGC
954 GCTGAGC
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
954 GCTGAGC
BstUI 781 CGCG CG^CG
1096 CGCG
Cac8I 702 GCCAGC GC[ACGT]^[ACGT]GC
959 GCTGGC
CelII 15 GCTGAGC GC^T[ACGT]AGC
954 GCTGAGC
CfoI 13 GCGC GCG^C
780 GCGC
FauI 687 CCCGC CCCGC(4/6)
911 GCGGG
HhaI 13 GCGC GCG^C
780 GCGC
HinP1I 13 GCGC G^CGC
780 GCGC
MaeI 160 CTAG C^TAG
250 CTAG
MvnI 781 CGCG CG^CG
1096 CGCG
NciI 945 CCGGG CC^[GC]GG
1146 CCCGG
TaqI 584 TCGA T^CGA
734 TCGA
TthHB8I 584 TCGA T^CGA
734 TCGA
XmnI 289 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
812 GAAACATTTC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI Ksp632I MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI Tsp45I TspRI