Restriction map for
pF1KB9550
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BcnI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 712 GTAGAC GT^[AC][GT]AC
AclI 763 AACGTT AA^CGTT
AcsI 48 AAATTT [GA]^AATT[CT]
AfaI 65 GTAC GT^AC
AflIII 491 ACGTGT A^C[GA][CT]GT
AocI 429 CCTCAGG CC^T[ACGT]AGG
ApoI 48 AAATTT [GA]^AATT[CT]
Asp700I 595 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 576 GGACC G^G[AT]CC
BanI 690 GGTGCC G^G[CT][GA]CC
BbvCI 823 GCTGAGG CCTCAGC(-5/-2)
BclI 873 TGATCA T^GATCA
BfaI 286 CTAG C^TAG
BglI 273 GCCCAATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 249 GGTCTC GGTCTC(1/5)
BsgI 92 CTGCAC GTGCAG(16/14)
BsmBI 816 CGTCTC CGTCTC(1/5)
BstAPI 170 GCACCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 429 CCTCAGG CC^T[ACGT]AGG
CfoI 837 GCGC GCG^C
DraII 575 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 577 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 531 TGGCCG [CT]^GGCC[GA]
EarI 97 CTCTTC CTCTTC(1/4)
Eco81I 429 CCTCAGG CC^T[ACGT]AGG
EcoO109I 575 GGGACCT [GA]G^G[ACGT]CC[CT]
FbaI 873 TGATCA T^GATCA
HhaI 837 GCGC GCG^C
HinP1I 837 GCGC G^CGC
Ksp632I 97 CTCTTC CTCTTC(1/4)
MaeI 286 CTAG C^TAG
NspI 755 GCATGC [GA]CATG^[CT]
PflMI 862 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 584 GAGTC GAGTC(4/5)
PpuMI 575 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 763 AACGTT AA^CGTT
RsaI 65 GTAC GT^AC
SphI 755 GCATGC GCATG^C
Van91I 862 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 595 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwI 618 GATCC GGATC(4/5)
222 GGATC
BseRI 412 CTCCTC GAGGAG(10/8)
826 GAGGAG
BsmAI 250 GTCTC GTCTC(1/5)
817 GTCTC
BstYI 222 GGATCT [GA]^GATC[CT]
617 AGATCC
Cfr13I 272 GGCCC G^G[ACGT]CC
576 GGACC
DsaI 213 CCATGG C^C[GA][CT]GG
245 CCATGG
HgaI 437 GCGTC GACGC(5/10)
815 GCGTC
HphI 871 GGTGA GGTGA(8/7)
37 TCACC
MflI 222 GGATCT [GA]^GATC[CT]
617 AGATCC
NcoI 213 CCATGG C^CATGG
245 CCATGG
PstI 199 CTGCAG CTGCA^G
588 CTGCAG
PvuII 114 CAGCTG CAG^CTG
622 CAGCTG
Sau96I 272 GGCCC G^G[ACGT]CC
576 GGACC
Tsp45I 12 GTCAC ^GT[GC]AC
19 GTCAC
XhoII 222 GGATCT [GA]^GATC[CT]
617 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV Sau3AI ScrFI SfaNI SfcI
SmlI StyI TfiI TseI Tsp509I
TspRI