Restriction map for
pF1KB9545
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BamHI BanI BbeI
BcgI BciVI BfrI BglI BglII
BlnI BpmI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CfoI ClaI CpoI
DraIII DrdI DsaI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI MvnI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AhdI 1437 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1373 GTGCAC G^TGCAC
ApaLI 1373 GTGCAC G^TGCAC
AspEI 1437 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 195 CCCGGG C^[CT]CG[GA]G
BbrPI 970 CACGTG CAC^GTG
BbsI 903 GAAGAC GAAGAC(2/6)
BbvCI 927 CCTCAGC CCTCAGC(-5/-2)
BclI 225 TGATCA T^GATCA
BfaI 1474 CTAG C^TAG
BpuAI 903 GAAGAC GAAGAC(2/6)
BsaAI 970 CACGTG [CT]AC^GT[GA]
BsaI 274 GAGACC GGTCTC(1/5)
BsiYI 1388 CCAAGTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1388 CCAAGTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 195 CCCGGG C^[CT]CG[GA]G
BspHI 270 TCATGA T^CATGA
BspMI 1030 ACCTGC ACCTGC(4/8)
BsrDI 452 CATTGC GCAATG(2/0)
BssSI 506 CTCGTG CACGAG(-5/-1)
BstBI 532 TTCGAA TT^CGAA
DraI 919 TTTAAA TTT^AAA
DraII 1157 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1437 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1157 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 225 TGATCA T^GATCA
HaeIII 1357 GGCC GG^CC
MaeI 1474 CTAG C^TAG
NaeI 1394 GCCGGC GCC^GGC
NgoMIV 1394 GCCGGC G^CCGGC
NspV 532 TTCGAA TT^CGAA
PmaCI 970 CACGTG CAC^GTG
PmlI 970 CACGTG CAC^GTG
PpuMI 1157 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 270 TCATGA T^CATGA
SacI 85 GAGCTC GAGCT^C
SfuI 532 TTCGAA TT^CGAA
SmaI 195 CCCGGG CCC^GGG
XmaCI 195 CCCGGG C^CCGGG
XmaI 195 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 470 GTAC GT^AC
647 GTAC
AflIII 108 ACATGT A^C[GA][CT]GT
1340 ACATGT
AlwI 823 GATCC GGATC(4/5)
1537 GGATC
AvaII 1158 GGTCC G^G[AT]CC
1179 GGACC
BanII 85 GAGCTC G[GA]GC[CT]^C
1108 GAGCCC
BcnI 195 CCCGG CC^[GC]GG
196 CCGGG
BlpI 82 GCTGAGC GC^T[ACGT]AGC
124 GCTGAGC
Bpu1102I 82 GCTGAGC GC^T[ACGT]AGC
124 GCTGAGC
BsmAI 274 GAGAC GTCTC(1/5)
1310 GTCTC
BsmFI 1301 GTCCC GGGAC(10/14)
1623 GTCCC
BspLU11I 108 ACATGT A^CATGT
1340 ACATGT
BsrFI 1046 ACCGGC [GA]^CCGG[CT]
1394 GCCGGC
CelII 82 GCTGAGC GC^T[ACGT]AGC
124 GCTGAGC
Cfr10I 1046 ACCGGC [GA]^CCGG[CT]
1394 GCCGGC
MaeII 971 ACGT A^CGT
1270 ACGT
MspA1I 930 CAGCTG C[AC]G^C[GT]G
1011 CAGCTG
NciI 195 CCCGG CC^[GC]GG
196 CCGGG
NspI 108 ACATGT [GA]CATG^[CT]
1340 ACATGT
PvuII 930 CAGCTG CAG^CTG
1011 CAGCTG
RsaI 470 GTAC GT^AC
647 GTAC
SfcI 1072 CTACAG C^T[GA][CT]AG
1399 CTACAG
XcmI 681 CCATGTTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1182 CCATCATAAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AluI ApoI Asp700I AspHI
BbvI BmyI BsaJI BsiHKAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HapII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI