Restriction map for
pF1KB9544
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccIII AciI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BalI
BamHI BanI BbeI BbrPI BbsI
BcgI BciVI BfrI BglII BlpI
Bpu1102I BpuAI BsaAI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 312 GTCTAC GT^[AC][GT]AC
AccII 1264 CGCG CG^CG
AclI 101 AACGTT AA^CGTT
AcsI 156 GAATTC [GA]^AATT[CT]
AcyI 808 GACGCC G[GA]^CG[CT]C
AfaI 1483 GTAC GT^AC
AflIII 1229 ACATGT A^C[GA][CT]GT
AlwNI 1381 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 156 GAATTC [GA]^AATT[CT]
Asp700I 862 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 436 GAGCAC G[AT]GC[AT]^C
AspI 1616 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 683 CTCGGG C^[CT]CG[GA]G
AvaII 330 GGACC G^G[AT]CC
AvrII 708 CCTAGG C^CTAGG
BanII 1558 GAGCCC G[GA]GC[CT]^C
BbvCI 1324 GCTGAGG CCTCAGC(-5/-2)
BclI 45 TGATCA T^GATCA
BglI 788 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 708 CCTAGG C^CTAGG
BpmI 1106 CTGGAG CTGGAG(16/14)
BsaBI 40 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 808 GACGCC G[GA]^CG[CT]C
BsiHKAI 436 GAGCAC G[AT]GC[AT]^C
BsoBI 683 CTCGGG C^[CT]CG[GA]G
BspLU11I 1229 ACATGT A^CATGT
BsrDI 1779 CATTGC GCAATG(2/0)
BstUI 1264 CGCG CG^CG
BstXI 1143 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 567 TTTAAA TTT^AAA
EaeI 78 TGGCCG [CT]^GGCC[GA]
EcoRI 156 GAATTC G^AATTC
FbaI 45 TGATCA T^GATCA
Hin1I 808 GACGCC G[GA]^CG[CT]C
MaeII 102 ACGT A^CGT
MamI 40 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1264 CGCG CG^CG
PflFI 1616 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 101 AACGTT AA^CGTT
RsaI 1483 GTAC GT^AC
ScaI 1482 AGTACT AGT^ACT
SexAI 1241 ACCAGGT A^CC[AT]GGT
TaqI 505 TCGA T^CGA
Tth111I 1616 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 505 TCGA T^CGA
XmnI 862 GAACCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BcnI 743 CCCGG CC^[GC]GG
1795 CCGGG
Cac8I 76 GCTGGC GC[ACGT]^[ACGT]GC
364 GCAGGC
DpnII 46 GATC ^GATC
847 GATC
HapII 744 CCGG C^CGG
1795 CCGG
HgaI 769 GACGC GACGC(5/10)
808 GACGC
HpaII 744 CCGG C^CGG
1795 CCGG
MboI 46 GATC ^GATC
847 GATC
MseI 121 TTAA T^TAA
568 TTAA
MspA1I 74 CAGCTG C[AC]G^C[GT]G
1585 CAGCTG
MspI 744 CCGG C^CGG
1795 CCGG
NciI 743 CCCGG CC^[GC]GG
1795 CCGGG
NdeII 46 GATC ^GATC
847 GATC
NspI 1229 ACATGT [GA]CATG^[CT]
1708 GCATGT
PflMI 1099 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1129 CCACTTCCTGG
PstI 1387 CTGCAG CTGCA^G
1624 CTGCAG
PvuII 74 CAGCTG CAG^CTG
1585 CAGCTG
SapI 969 GAAGAGC GCTCTTC(1/4)
407 GCTCTTC
Sau3AI 46 GATC ^GATC
847 GATC
SfaNI 1094 GCATC GCATC(5/9)
1435 GCATC
Tru9I 121 TTAA T^TAA
568 TTAA
Van91I 1099 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1129 CCACTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cfr13I DdeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MslI MvaI MwoI NlaIII
NlaIV PleI Sau96I ScrFI SfcI
StyI TfiI TseI Tsp45I Tsp509I
TspRI