Restriction map for
pF1KB9542
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NarI NciI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AgeI 791 ACCGGT A^CCGGT
AlwNI 640 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 516 AGCGCT AGC^GCT
AspHI 779 GTGCTC G[AT]GC[AT]^C
BanI 763 GGTGCC G^G[CT][GA]CC
BfaI 128 CTAG C^TAG
BglI 393 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 474 AGATCT A^GATCT
BmyI 779 GTGCTC G[AGT]GC[ACT]^C
BpmI 709 CTGGAG CTGGAG(16/14)
BsaWI 791 ACCGGT [AT]^CCGG[AT]
BseRI 826 GAGGAG GAGGAG(10/8)
BsiHKAI 779 GTGCTC G[AT]GC[AT]^C
BsmBI 18 GAGACG CGTCTC(1/5)
Bsp1286I 779 GTGCTC G[AGT]GC[ACT]^C
BsrI 212 CCAGT ACTGG(1/-1)
BssSI 823 CACGAG CACGAG(-5/-1)
BstEII 634 GGTTACC G^GT[ACGT]ACC
BstPI 634 GGTTACC G^GT[ACGT]ACC
BstYI 474 AGATCT [GA]^GATC[CT]
CfoI 517 GCGC GCG^C
DpnII 475 GATC ^GATC
DraII 688 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 652 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 615 CCATGG C^C[GA][CT]GG
EarI 306 GAAGAG CTCTTC(1/4)
Eco47III 516 AGCGCT AGC^GCT
EcoNI 412 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 688 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 634 GGTTACC G^GT[ACGT]ACC
EcoT14I 615 CCATGG C^C[AT][AT]GG
HaeII 516 AGCGCT [GA]GCGC^[CT]
HhaI 517 GCGC GCG^C
HinP1I 517 GCGC G^CGC
HindIII 232 AAGCTT A^AGCTT
HphI 110 TCACC GGTGA(8/7)
Ksp632I 306 GAAGAG CTCTTC(1/4)
MaeI 128 CTAG C^TAG
MaeIII 635 GTTAC ^GT[ACGT]AC
MboI 475 GATC ^GATC
MflI 474 AGATCT [GA]^GATC[CT]
NaeI 102 GCCGGC GCC^GGC
NcoI 615 CCATGG C^CATGG
NdeII 475 GATC ^GATC
NgoMIV 102 GCCGGC G^CCGGC
PinAI 791 ACCGGT A^CCGGT
PpuMI 688 GGGACCC [GA]G^G[AT]CC[CT]
PstI 413 CTGCAG CTGCA^G
Sau3AI 475 GATC ^GATC
SbfI 412 CCTGCAGG CCTGCA^GG
SfcI 413 CTGCAG C^T[GA][CT]AG
Sse8387I 412 CCTGCAGG CCTGCA^GG
StuI 409 AGGCCT AGG^CCT
StyI 615 CCATGG C^C[AT][AT]GG
TaqI 304 TCGA T^CGA
Tsp509I 207 AATT ^AATT
TthHB8I 304 TCGA T^CGA
XcmI 113 CCAACATCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 474 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 468 CCGC CCGC(-3/-1)
246 GCGG
AflIII 133 ACATGT A^C[GA][CT]GT
498 ACATGT
AseI 151 ATTAAT AT^TAAT
204 ATTAAT
AsnI 151 ATTAAT AT^TAAT
204 ATTAAT
AvaII 689 GGACC G^G[AT]CC
789 GGACC
BsaI 23 GAGACC GGTCTC(1/5)
619 GGTCTC
BspLU11I 133 ACATGT A^CATGT
498 ACATGT
BsrFI 102 GCCGGC [GA]^CCGG[CT]
791 ACCGGT
BstXI 525 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
667 CCAATGCTGTGG
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
791 ACCGGT
DdeI 241 CTCAG C^T[ACGT]AG
782 CTCAG
HapII 103 CCGG C^CGG
792 CCGG
HpaII 103 CCGG C^CGG
792 CCGG
MboII 255 GAAGA GAAGA(8/7)
306 GAAGA
MslI 117 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
272 CACCACCATG
MspI 103 CCGG C^CGG
792 CCGG
NspI 133 ACATGT [GA]CATG^[CT]
498 ACATGT
PflMI 113 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCACGCTGTGG
PleI 47 GACTC GAGTC(4/5)
97 GACTC
PshBI 151 ATTAAT AT^TAAT
204 ATTAAT
SfaNI 32 GCATC GCATC(5/9)
422 GCATC
SmlI 478 CTTGAG C^T[CT][GA]AG
838 CTCAAG
TfiI 328 GATTC G^A[AT]TC
678 GAATC
TspRI 332 CAGTG CA[GC]TG[ACGT][ACGT]^
531 CAGTG
Van91I 113 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
651 CCACGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BsmAI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MnlI
MseI MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI Tru9I TseI