Restriction map for
pF1KB9451
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
ApaLI ApoI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AvrII
BamHI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI HincII HindII
HindIII HpaI KpnI Ksp632I MaeI
MaeIII MamI MboI MfeI MflI
MluI MroI MseI MslI MunI
NcoI NdeI NdeII NheI NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SapI Sau3AI SbfI ScaI SexAI
SfaNI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Aor51HI 467 AGCGCT AGC^GCT
ApaI 856 GGGCCC GGGCC^C
AscI 862 GGCGCGCC GG^CGCGCC
Asp700I 483 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 928 TGCGCA TGC^GCA
BalI 108 TGGCCA TGG^CCA
BbrPI 112 CACGTG CAC^GTG
BbvCI 550 GCTGAGG CCTCAGC(-5/-2)
BlpI 495 GCTGAGC GC^T[ACGT]AGC
BpmI 533 CTGGAG CTGGAG(16/14)
Bpu1102I 495 GCTGAGC GC^T[ACGT]AGC
BsaAI 112 CACGTG [CT]AC^GT[GA]
BsgI 960 GTGCAG GTGCAG(16/14)
BsrFI 267 GCCGGC [GA]^CCGG[CT]
BsrI 9 CCAGT ACTGG(1/-1)
BssHII 863 GCGCGC G^CGCGC
BstXI 782 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 495 GCTGAGC GC^T[ACGT]AGC
Cfr10I 267 GCCGGC [GA]^CCGG[CT]
Eco47III 467 AGCGCT AGC^GCT
FspI 928 TGCGCA TGC^GCA
HinfI 151 GAGTC G^A[ACGT]TC
MaeII 113 ACGT A^CGT
MluNI 108 TGGCCA TGG^CCA
MscI 108 TGGCCA TGG^CCA
NaeI 267 GCCGGC GCC^GGC
NgoMIV 267 GCCGGC G^CCGGC
PleI 151 GAGTC GAGTC(4/5)
PmaCI 112 CACGTG CAC^GTG
PmlI 112 CACGTG CAC^GTG
PstI 172 CTGCAG CTGCA^G
PvuII 599 CAGCTG CAG^CTG
SfcI 172 CTGCAG C^T[GA][CT]AG
StuI 52 AGGCCT AGG^CCT
TaqI 394 TCGA T^CGA
TthHB8I 394 TCGA T^CGA
XmnI 483 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaI 286 CCCGAG C^[CT]CG[GA]G
955 CCCGAG
BsaI 445 GAGACC GGTCTC(1/5)
760 GAGACC
BsmAI 445 GAGAC GTCTC(1/5)
760 GAGAC
BsoBI 286 CCCGAG C^[CT]CG[GA]G
955 CCCGAG
BsrBI 228 GAGCGG CCGCTC(-3/-3)
498 GAGCGG
BstNI 55 CCTGG CC^[AT]GG
717 CCAGG
DdeI 496 CTGAG C^T[ACGT]AG
551 CTGAG
DraII 317 AGGCCCC [GA]G^G[ACGT]CC[CT]
839 AGGCCCC
EcoO109I 317 AGGCCCC [GA]G^G[ACGT]CC[CT]
839 AGGCCCC
EcoRII 55 CCTGG ^CC[AT]GG
717 CCAGG
HphI 262 GGTGA GGTGA(8/7)
544 GGTGA
MboII 516 GAAGA GAAGA(8/7)
400 TCTTC
MvaI 55 CCTGG CC^[AT]GG
717 CCAGG
SfiI 375 GGCCGCGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
804 GGCCGAGGAGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BanII BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BseRI BsiEI
BsiYI BslI Bsp1286I BssKI BstUI
Cac8I CfoI Cfr13I DsaI EaeI
EagI EclXI Eco52I EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MnlI MspA1I MspI
MvnI MwoI NarI NciI NlaIV
NotI SacII Sau96I ScrFI SfoI
StyI TseI