Restriction map for
pF1KB9445
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV FauI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MslI MspA1I
MunI MvnI NaeI NarI NgoMIV
NotI NruI NspI NspV PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AciI 1527 GCGG CCGC(-3/-1)
AseI 1110 ATTAAT AT^TAAT
AsnI 1110 ATTAAT AT^TAAT
BalI 78 TGGCCA TGG^CCA
BanII 131 GGGCTC G[GA]GC[CT]^C
BbvI 1403 GCAGC GCAGC(8/12)
BciVI 1563 GTATCC GTATCC(6/5)
BclI 1443 TGATCA T^GATCA
BcnI 129 CCGGG CC^[GC]GG
BglII 227 AGATCT A^GATCT
BmyI 131 GGGCTC G[AGT]GC[ACT]^C
BsgI 928 GTGCAG GTGCAG(16/14)
BsiEI 1043 CGGCCG CG[GA][CT]^CG
Bsp1286I 131 GGGCTC G[AGT]GC[ACT]^C
BspMI 1766 GCAGGT ACCTGC(4/8)
BsrDI 1825 GCAATG GCAATG(2/0)
BstAPI 688 GCACCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CfoI 388 GCGC GCG^C
DraI 1957 TTTAAA TTT^AAA
DraII 663 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 689 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1039 GACTCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 81 CCATGG C^C[GA][CT]GG
EagI 1043 CGGCCG C^GGCCG
EclXI 1043 CGGCCG C^GGCCG
Eco52I 1043 CGGCCG C^GGCCG
EcoO109I 663 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 81 CCATGG C^C[AT][AT]GG
EcoT22I 1790 ATGCAT ATGCA^T
FbaI 1443 TGATCA T^GATCA
Fnu4HI 1403 GCAGC GC^[ACGT]GC
HapII 129 CCGG C^CGG
HhaI 388 GCGC GCG^C
HinP1I 388 GCGC G^CGC
HincII 1855 GTTGAC GT[CT]^[GA]AC
HindII 1855 GTTGAC GT[CT]^[GA]AC
HpaII 129 CCGG C^CGG
ItaI 1403 GCAGC GC^[ACGT]GC
MluNI 78 TGGCCA TGG^CCA
MscI 78 TGGCCA TGG^CCA
MspI 129 CCGG C^CGG
NciI 129 CCGGG CC^[GC]GG
NcoI 81 CCATGG C^CATGG
NheI 697 GCTAGC G^CTAGC
NsiI 1790 ATGCAT ATGCA^T
PacI 301 TTAATTAA TTAAT^TAA
PshBI 1110 ATTAAT AT^TAAT
SapI 177 GCTCTTC GCTCTTC(1/4)
SmlI 1375 CTTGAG C^T[CT][GA]AG
StyI 81 CCATGG C^C[AT][AT]GG
TaqI 1282 TCGA T^CGA
TseI 1403 GCAGC G^C[AT]GC
TthHB8I 1282 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 662 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1259 CAGCTACTG
BbsI 115 GAAGAC GAAGAC(2/6)
1695 GTCTTC
BpmI 1029 CTCCAG CTGGAG(16/14)
1874 CTCCAG
BpuAI 115 GAAGAC GAAGAC(2/6)
1695 GTCTTC
BsmAI 616 GAGAC GTCTC(1/5)
1530 GAGAC
BstXI 468 CCAAGATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1909 CCAATGACCTGG
BstYI 227 AGATCT [GA]^GATC[CT]
1725 AGATCC
Cac8I 697 GCTAGC GC[ACGT]^[ACGT]GC
1523 GCTTGC
EaeI 78 TGGCCA [CT]^GGCC[GA]
1043 CGGCCG
EcoNI 1354 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1873 CCTCCAGTAGG
HindIII 1521 AAGCTT A^AGCTT
1744 AAGCTT
HphI 427 GGTGA GGTGA(8/7)
1312 GGTGA
MflI 227 AGATCT [GA]^GATC[CT]
1725 AGATCC
MwoI 688 GCACCTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
930 GCAGAATCAGC
NdeI 901 CATATG CA^TATG
1505 CATATG
NlaIV 1065 GGTCCC GG[ACGT]^[ACGT]CC
1882 GGGACC
ScaI 38 AGTACT AGT^ACT
1981 AGTACT
SfaNI 1122 GATGC GCATC(5/9)
374 GCATC
SfcI 26 CTACAG C^T[GA][CT]AG
255 CTATAG
SspI 142 AATATT AAT^ATT
835 AATATT
Tsp45I 360 GTCAC ^GT[GC]AC
428 GTGAC
XhoII 227 AGATCT [GA]^GATC[CT]
1725 AGATCC
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BfaI BsaJI BseRI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EarI
EcoRII FokI HaeIII HinfI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
PleI RsaI Sau3AI Sau96I ScrFI
TfiI Tru9I Tsp509I TspRI