Restriction map for
pF1KB9436
##################################
# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI HgaI
HincII HindII HindIII HinfI HpaI
KpnI KspI MaeI MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NdeII NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 141 GGCGCC G[GA]^CG[CT]C
AfaI 402 GTAC GT^AC
AviII 290 TGCGCA TGC^GCA
BanI 141 GGCGCC G^G[CT][GA]CC
BanII 327 GAGCCC G[GA]GC[CT]^C
BbeI 141 GGCGCC GGCGC^C
BbvCI 97 CCTCAGC CCTCAGC(-5/-2)
BmyI 327 GAGCCC G[AGT]GC[ACT]^C
BsaHI 141 GGCGCC G[GA]^CG[CT]C
BseRI 95 CTCCTC GAGGAG(10/8)
BsmFI 112 GTCCC GGGAC(10/14)
Bsp1286I 327 GAGCCC G[AGT]GC[ACT]^C
BspMI 159 ACCTGC ACCTGC(4/8)
BstAPI 345 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 421 TTCGAA TT^CGAA
DraII 254 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 145 CCACGG C^C[GA][CT]GG
EarI 178 GAAGAG CTCTTC(1/4)
EcoO109I 254 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 431 CCAAGG C^C[AT][AT]GG
EcoT22I 222 ATGCAT ATGCA^T
FauI 164 CCCGC CCCGC(4/6)
FspI 290 TGCGCA TGC^GCA
HaeII 141 GGCGCC [GA]GCGC^[CT]
Hin1I 141 GGCGCC G[GA]^CG[CT]C
HphI 157 TCACC GGTGA(8/7)
KasI 141 GGCGCC G^GCGCC
Ksp632I 178 GAAGAG CTCTTC(1/4)
MaeII 55 ACGT A^CGT
MaeIII 156 GTCAC ^GT[ACGT]AC
MboII 178 GAAGA GAAGA(8/7)
NaeI 138 GCCGGC GCC^GGC
NarI 141 GGCGCC GG^CGCC
NgoMIV 138 GCCGGC G^CCGGC
NsiI 222 ATGCAT ATGCA^T
NspI 416 GCATGT [GA]CATG^[CT]
NspV 421 TTCGAA TT^CGAA
PpuMI 254 AGGACCT [GA]G^G[AT]CC[CT]
PstI 352 CTGCAG CTGCA^G
PvuII 349 CAGCTG CAG^CTG
RsaI 402 GTAC GT^AC
SfcI 352 CTGCAG C^T[GA][CT]AG
SfoI 141 GGCGCC GGC^GCC
SfuI 421 TTCGAA TT^CGAA
SgrAI 137 CGCCGGCG C[GA]^CCGG[CT]G
StyI 431 CCAAGG C^C[AT][AT]GG
TaqI 422 TCGA T^CGA
Tsp45I 156 GTCAC ^GT[GC]AC
TthHB8I 422 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 166 CGCG CG^CG
168 CGCG
BsaJI 145 CCACGG C^C[ACGT][ACGT]GG
431 CCAAGG
BsrFI 108 GCCGGT [GA]^CCGG[CT]
138 GCCGGC
BstF5I 318 CATCC GGATG(2/0)
39 GGATG
BstNI 252 CCAGG CC^[AT]GG
282 CCAGG
BstUI 166 CGCG CG^CG
168 CGCG
Cfr10I 108 GCCGGT [GA]^CCGG[CT]
138 GCCGGC
EcoRII 252 CCAGG ^CC[AT]GG
282 CCAGG
FokI 318 CATCC GGATG(9/13)
39 GGATG
HaeIII 3 GGCC GG^CC
149 GGCC
MvaI 252 CCAGG CC^[AT]GG
282 CCAGG
MvnI 166 CGCG CG^CG
168 CGCG
NlaIV 111 GGTCCC GG[ACGT]^[ACGT]CC
141 GGCGCC
SfaNI 288 GATGC GCATC(5/9)
317 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BsiYI BslI BssKI Cac8I
CfoI Cfr13I DdeI Fnu4HI HapII
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MwoI NciI NlaIII
Sau96I ScrFI TseI