Restriction map for
pF1KB9273
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
BstBI BstXI CelII Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SmlI SphI Sse8387I SspI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 783 GTATAC GT^[AC][GT]AC
AflIII 863 ACGTGT A^C[GA][CT]GT
Alw44I 928 GTGCAC G^TGCAC
AlwI 1063 GATCC GGATC(4/5)
AlwNI 703 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 552 CCTGAGG CC^T[ACGT]AGG
ApaLI 928 GTGCAC G^TGCAC
AspHI 928 GTGCAC G[AT]GC[AT]^C
BalI 1122 TGGCCA TGG^CCA
BanII 823 GAGCCC G[GA]GC[CT]^C
BbsI 742 GAAGAC GAAGAC(2/6)
BfaI 1015 CTAG C^TAG
BglI 438 GCCCGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 185 AGATCT A^GATCT
BpuAI 742 GAAGAC GAAGAC(2/6)
BsiHKAI 928 GTGCAC G[AT]GC[AT]^C
Bsp1407I 899 TGTACA T^GTACA
BspMI 908 ACCTGC ACCTGC(4/8)
BsrDI 366 CATTGC GCAATG(2/0)
BsrGI 899 TGTACA T^GTACA
Bst1107I 783 GTATAC GTA^TAC
BstAPI 3 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 82 GGTTACC G^GT[ACGT]ACC
BstPI 82 GGTTACC G^GT[ACGT]ACC
BstZ17I 783 GTATAC GTA^TAC
Bsu36I 552 CCTGAGG CC^T[ACGT]AGG
DraI 508 TTTAAA TTT^AAA
DraII 905 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 16 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 53 CCGTGG C^C[GA][CT]GG
EarI 675 GAAGAG CTCTTC(1/4)
Eco81I 552 CCTGAGG CC^T[ACGT]AGG
EcoO109I 905 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 82 GGTTACC G^GT[ACGT]ACC
EcoT14I 788 CCTTGG C^C[AT][AT]GG
HaeII 49 AGCGCC [GA]GCGC^[CT]
HgaI 851 GCGTC GACGC(5/10)
Ksp632I 675 GAAGAG CTCTTC(1/4)
MaeI 1015 CTAG C^TAG
MluNI 1122 TGGCCA TGG^CCA
MscI 1122 TGGCCA TGG^CCA
MslI 654 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 659 GCATGT [GA]CATG^[CT]
PflMI 1002 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 905 GGGACCT [GA]G^G[AT]CC[CT]
PstI 709 CTGCAG CTGCA^G
PvuII 497 CAGCTG CAG^CTG
SfcI 709 CTGCAG C^T[GA][CT]AG
SnaBI 217 TACGTA TAC^GTA
SpeI 1014 ACTAGT A^CTAGT
SspBI 899 TGTACA T^GTACA
StuI 5 AGGCCT AGG^CCT
StyI 788 CCTTGG C^C[AT][AT]GG
TaqI 717 TCGA T^CGA
TfiI 995 GATTC G^A[AT]TC
TthHB8I 717 TCGA T^CGA
Van91I 1002 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 60 CGCG CG^CG
925 CGCG
AcsI 316 AAATTT [GA]^AATT[CT]
681 GAATTT
ApoI 316 AAATTT [GA]^AATT[CT]
681 GAATTT
AspI 719 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACAGCGTC
BbvCI 69 GCTGAGG CCTCAGC(-5/-2)
912 GCTGAGG
BcnI 878 CCCGG CC^[GC]GG
966 CCGGG
BmyI 823 GAGCCC G[AGT]GC[ACT]^C
928 GTGCAC
BpmI 490 CTCCAG CTGGAG(16/14)
1094 CTCCAG
BsaAI 217 TACGTA [CT]AC^GT[GA]
832 TACGTG
Bsp1286I 823 GAGCCC G[AGT]GC[ACT]^C
928 GTGCAC
BstUI 60 CGCG CG^CG
925 CGCG
BstYI 185 AGATCT [GA]^GATC[CT]
1062 AGATCC
DpnII 186 GATC ^GATC
1063 GATC
EaeI 56 TGGCCG [CT]^GGCC[GA]
1122 TGGCCA
FauI 952 CCCGC CCCGC(4/6)
1056 GCGGG
HphI 90 GGTGA GGTGA(8/7)
859 GGTGA
MboI 186 GATC ^GATC
1063 GATC
MflI 185 AGATCT [GA]^GATC[CT]
1062 AGATCC
MspA1I 497 CAGCTG C[AC]G^C[GT]G
1054 CAGCGG
MvnI 60 CGCG CG^CG
925 CGCG
NciI 878 CCCGG CC^[GC]GG
966 CCGGG
NdeII 186 GATC ^GATC
1063 GATC
PflFI 719 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACAGCGTC
PleI 118 GACTC GAGTC(4/5)
279 GACTC
Sau3AI 186 GATC ^GATC
1063 GATC
SexAI 86 ACCAGGT A^CC[AT]GGT
720 ACCTGGT
Tsp509I 317 AATT ^AATT
682 AATT
Tth111I 719 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
847 GACAGCGTC
XcmI 563 CCATCTTCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1105 CCAGCGCAGAGCTGG
XhoII 185 AGATCT [GA]^GATC[CT]
1062 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BsaJI BseRI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboII MnlI MseI MspI MvaI
MwoI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI Tru9I TseI Tsp45I
TspRI