Restriction map for
pF1KB9100
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII Alw44I
Aor51HI ApaLI AscI Asp718I AviII
AvrII BalI BamHI BbvCI BcgI
BfrI BlnI BlpI Bpu1102I BsiEI
BsiWI BsmBI BsmI BspDI BspLU11I
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NruI
NspV PacI PmeI Psp1406I PvuI
RsrII SacI SacII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 888 CGCG CG^CG
AflIII 1394 ACGTGT A^C[GA][CT]GT
AgeI 950 ACCGGT A^CCGGT
AhdI 3016 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3151 CCTCAGG CC^T[ACGT]AGG
AseI 165 ATTAAT AT^TAAT
AsnI 165 ATTAAT AT^TAAT
Asp700I 1279 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3016 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2302 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 296 TGATCA T^GATCA
BglII 563 AGATCT A^GATCT
BsaBI 792 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2345 GAGACC GGTCTC(1/5)
BstAPI 1968 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 888 CGCG CG^CG
Bsu36I 3151 CCTCAGG CC^T[ACGT]AGG
DrdI 2713 GACCTGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3016 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3151 CCTCAGG CC^T[ACGT]AGG
EcoNI 2869 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2554 GAATTC G^AATTC
EcoT22I 1408 ATGCAT ATGCA^T
FbaI 296 TGATCA T^GATCA
MamI 792 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 888 CGCG CG^CG
NaeI 974 GCCGGC GCC^GGC
NgoMIV 974 GCCGGC G^CCGGC
NsiI 1408 ATGCAT ATGCA^T
NspI 1410 GCATGC [GA]CATG^[CT]
PaeR7I 2096 CTCGAG C^TCGAG
PflFI 2302 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 950 ACCGGT A^CCGGT
PshAI 408 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 165 ATTAAT AT^TAAT
PvuII 2008 CAGCTG CAG^CTG
SalI 3272 GTCGAC G^TCGAC
SphI 1410 GCATGC GCATG^C
Tth111I 2302 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2096 CTCGAG C^TCGAG
XmnI 1279 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 485 TCCGGA T^CCGGA
1638 TCCGGA
ApaI 2403 GGGCCC GGGCC^C
2658 GGGCCC
BbeI 652 GGCGCC GGCGC^C
1667 GGCGCC
BbrPI 421 CACGTG CAC^GTG
1018 CACGTG
BbsI 2589 GAAGAC GAAGAC(2/6)
2542 GTCTTC
BciVI 2878 GGATAC GTATCC(6/5)
1531 GTATCC
BglI 716 GCCTCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1932 GCCTTCTTGGC
BpuAI 2589 GAAGAC GAAGAC(2/6)
2542 GTCTTC
BsaAI 421 CACGTG [CT]AC^GT[GA]
1018 CACGTG
BseAI 485 TCCGGA T^CCGGA
1638 TCCGGA
Bsp1407I 284 TGTACA T^GTACA
2963 TGTACA
BspEI 485 TCCGGA T^CCGGA
1638 TCCGGA
BsrBI 233 GAGCGG CCGCTC(-3/-3)
616 GAGCGG
BsrFI 950 ACCGGT [GA]^CCGG[CT]
974 GCCGGC
BsrGI 284 TGTACA T^GTACA
2963 TGTACA
Cfr10I 950 ACCGGT [GA]^CCGG[CT]
974 GCCGGC
DsaI 1121 CCATGG C^C[GA][CT]GG
2972 CCATGG
HaeII 652 GGCGCC [GA]GCGC^[CT]
1667 GGCGCC
KasI 652 GGCGCC G^GCGCC
1667 GGCGCC
MroI 485 TCCGGA T^CCGGA
1638 TCCGGA
MspA1I 451 CCGCTG C[AC]G^C[GT]G
2008 CAGCTG
NarI 652 GGCGCC GG^CGCC
1667 GGCGCC
NcoI 1121 CCATGG C^CATGG
2972 CCATGG
PmaCI 421 CACGTG CAC^GTG
1018 CACGTG
PmlI 421 CACGTG CAC^GTG
1018 CACGTG
PpuMI 2711 GGGACCT [GA]G^G[AT]CC[CT]
3056 AGGTCCC
ScaI 1316 AGTACT AGT^ACT
2479 AGTACT
SfoI 652 GGCGCC GGC^GCC
1667 GGCGCC
SmaI 1052 CCCGGG CCC^GGG
1082 CCCGGG
SspBI 284 TGTACA T^GTACA
2963 TGTACA
SspI 1179 AATATT AAT^ATT
1823 AATATT
StuI 461 AGGCCT AGG^CCT
1040 AGGCCT
XmaCI 1052 CCCGGG C^CCGGG
1082 CCCGGG
XmaI 1052 CCCGGG C^CCGGG
1082 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII