Restriction map for
pF1KB9016
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
AluI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BbvCI BcgI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HgaI HindIII HpaI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp45I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 352 GGCGCC G[GA]^CG[CT]C
AfaI 489 GTAC GT^AC
ApaI 604 GGGCCC GGGCC^C
BanI 352 GGCGCC G^G[CT][GA]CC
BanII 604 GGGCCC G[GA]GC[CT]^C
BbeI 352 GGCGCC GGCGC^C
BbrPI 79 CACGTG CAC^GTG
BbsI 774 GAAGAC GAAGAC(2/6)
BciVI 571 GGATAC GTATCC(6/5)
BclI 75 TGATCA T^GATCA
BcnI 165 CCGGG CC^[GC]GG
BglI 497 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 604 GGGCCC G[AGT]GC[ACT]^C
BpuAI 774 GAAGAC GAAGAC(2/6)
BsaHI 352 GGCGCC G[GA]^CG[CT]C
BsgI 577 GTGCAG GTGCAG(16/14)
Bsp1286I 604 GGGCCC G[AGT]GC[ACT]^C
BssSI 729 CACGAG CACGAG(-5/-1)
DdeI 530 CTGAG C^T[ACGT]AG
FbaI 75 TGATCA T^GATCA
Hin1I 352 GGCGCC G[GA]^CG[CT]C
HincII 333 GTTGAC GT[CT]^[GA]AC
HindII 333 GTTGAC GT[CT]^[GA]AC
HinfI 15 GAATC G^A[ACGT]TC
HphI 406 GGTGA GGTGA(8/7)
KasI 352 GGCGCC G^GCGCC
MslI 60 CATCCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 352 GGCGCC GG^CGCC
NciI 165 CCGGG CC^[GC]GG
PmaCI 79 CACGTG CAC^GTG
PmlI 79 CACGTG CAC^GTG
PpuMI 543 AGGACCC [GA]G^G[AT]CC[CT]
PstI 650 CTGCAG CTGCA^G
RsaI 489 GTAC GT^AC
SfcI 650 CTGCAG C^T[GA][CT]AG
SfoI 352 GGCGCC GGC^GCC
SmlI 392 CTCAAG C^T[CT][GA]AG
TfiI 15 GAATC G^A[AT]TC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 148 GTCTAC GT^[AC][GT]AC
718 GTCTAC
AvaII 131 GGACC G^G[AT]CC
544 GGACC
BpmI 379 CTCCAG CTGGAG(16/14)
399 CTCCAG
BsaAI 79 CACGTG [CT]AC^GT[GA]
574 TACGTG
BsaWI 618 TCCGGT [AT]^CCGG[AT]
762 ACCGGA
BspHI 209 TCATGA T^CATGA
227 TCATGA
BsrI 416 CCAGT ACTGG(1/-1)
472 CCAGT
Cac8I 682 GCCAGC GC[ACGT]^[ACGT]GC
734 GCCAGC
DraII 451 GGGGCCT [GA]G^G[ACGT]CC[CT]
543 AGGACCC
DsaI 566 CCATGG C^C[GA][CT]GG
608 CCATGG
EcoO109I 451 GGGGCCT [GA]G^G[ACGT]CC[CT]
543 AGGACCC
NcoI 566 CCATGG C^CATGG
608 CCATGG
NspI 440 ACATGC [GA]CATG^[CT]
722 ACATGC
RcaI 209 TCATGA T^CATGA
227 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BsmFI BssKI BstF5I BstNI CfoI
Cfr13I DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI StyI
TseI TspRI