Restriction map for
pF1KB9001
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AlwI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Cfr10I ClaI CpoI DpnII
DraIII DrdI DsaI EagI EclXI
Eco52I EcoNI EcoO65I EcoRV EcoT14I
FbaI FseI FspI HpaI KpnI
KspI MamI MboI MflI MluI
MroI MslI NaeI NcoI NdeII
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 126 CGCG CG^CG
AflIII 562 ACACGT A^C[GA][CT]GT
AhdI 886 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1213 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 580 CCTGAGG CC^T[ACGT]AGG
Aor51HI 71 AGCGCT AGC^GCT
Asp700I 649 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 886 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 853 GAGCAC G[AT]GC[AT]^C
BanI 993 GGCGCC G^G[CT][GA]CC
BbeI 993 GGCGCC GGCGC^C
BbsI 271 GTCTTC GAAGAC(2/6)
BcnI 213 CCGGG CC^[GC]GG
BlpI 168 GCTCAGC GC^T[ACGT]AGC
BpmI 292 CTGGAG CTGGAG(16/14)
Bpu1102I 168 GCTCAGC GC^T[ACGT]AGC
BpuAI 271 GTCTTC GAAGAC(2/6)
BsgI 1139 GTGCAG GTGCAG(16/14)
BsiHKAI 853 GAGCAC G[AT]GC[AT]^C
BspMI 389 ACCTGC ACCTGC(4/8)
BstUI 126 CGCG CG^CG
BstXI 426 CCATTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 580 CCTGAGG CC^T[ACGT]AGG
CelII 168 GCTCAGC GC^T[ACGT]AGC
DraI 453 TTTAAA TTT^AAA
DraII 1257 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 886 GACTTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 71 AGCGCT AGC^GCT
Eco81I 580 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1257 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 636 ATGCAT ATGCA^T
FauI 799 GCGGG CCCGC(4/6)
HapII 213 CCGG C^CGG
HgaI 940 GACGC GACGC(5/10)
HincII 16 GTTGAC GT[CT]^[GA]AC
HindII 16 GTTGAC GT[CT]^[GA]AC
HindIII 534 AAGCTT A^AGCTT
HpaII 213 CCGG C^CGG
KasI 993 GGCGCC G^GCGCC
MfeI 1043 CAATTG C^AATTG
MspA1I 842 CAGCGG C[AC]G^C[GT]G
MspI 213 CCGG C^CGG
MunI 1043 CAATTG C^AATTG
MvnI 126 CGCG CG^CG
NarI 993 GGCGCC GG^CGCC
NciI 213 CCGGG CC^[GC]GG
NdeI 551 CATATG CA^TATG
NsiI 636 ATGCAT ATGCA^T
NspI 195 ACATGC [GA]CATG^[CT]
PflMI 1212 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1257 AGGACCC [GA]G^G[AT]CC[CT]
SfcI 30 CTACAG C^T[GA][CT]AG
SfoI 993 GGCGCC GGC^GCC
SmlI 67 CTCAAG C^T[CT][GA]AG
Tsp45I 559 GTGAC ^GT[GC]AC
Van91I 1212 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 399 CCAAATGTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 649 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 739 GAATTC [GA]^AATT[CT]
1083 GAATTC
AcyI 940 GACGCC G[GA]^CG[CT]C
993 GGCGCC
AfaI 210 GTAC GT^AC
396 GTAC
ApoI 739 GAATTC [GA]^AATT[CT]
1083 GAATTC
AvaII 222 GGTCC G^G[AT]CC
1258 GGACC
BalI 155 TGGCCA TGG^CCA
515 TGGCCA
BanII 104 GGGCTC G[GA]GC[CT]^C
575 GAGCCC
BbvI 1141 GCAGC GCAGC(8/12)
1238 GCAGC
BfaI 721 CTAG C^TAG
904 CTAG
BsaHI 940 GACGCC G[GA]^CG[CT]C
993 GGCGCC
BseRI 1194 CTCCTC GAGGAG(10/8)
295 GAGGAG
BsmAI 47 GAGAC GTCTC(1/5)
1163 GTCTC
Cac8I 526 GCAGGC GC[ACGT]^[ACGT]GC
871 GCCAGC
CfoI 72 GCGC GCG^C
994 GCGC
EaeI 155 TGGCCA [CT]^GGCC[GA]
515 TGGCCA
EarI 820 CTCTTC CTCTTC(1/4)
835 CTCTTC
EcoRI 739 GAATTC G^AATTC
1083 GAATTC
HaeII 71 AGCGCT [GA]GCGC^[CT]
993 GGCGCC
HhaI 72 GCGC GCG^C
994 GCGC
Hin1I 940 GACGCC G[GA]^CG[CT]C
993 GGCGCC
HinP1I 72 GCGC G^CGC
994 GCGC
HphI 558 GGTGA GGTGA(8/7)
806 TCACC
Ksp632I 820 CTCTTC CTCTTC(1/4)
835 CTCTTC
MaeI 721 CTAG C^TAG
904 CTAG
MaeIII 464 GTAAC ^GT[ACGT]AC
559 GTGAC
MluNI 155 TGGCCA TGG^CCA
515 TGGCCA
MscI 155 TGGCCA TGG^CCA
515 TGGCCA
NlaIV 993 GGCGCC GG[ACGT]^[ACGT]CC
1258 GGACCC
RsaI 210 GTAC GT^AC
396 GTAC
TseI 1141 GCAGC G^C[AT]GC
1238 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeII MboII MnlI MseI
MvaI MwoI NlaIII PleI RcaI
Sau96I ScrFI SfaNI TfiI Tru9I
Tsp509I TspRI