Restriction map for
pF1KB9000
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I AspEI
AvrII BbrPI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SexAI SfuI SnaBI SpeI
SspBI SspI StuI SwaI Tru9I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 246 GACGTC GACGT^C
Acc65I 697 GGTACC G^GTACC
AflIII 1043 ACATGT A^C[GA][CT]GT
AlwNI 310 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 28 GGGCCC GGGCC^C
Asp718I 697 GGTACC G^GTACC
AspHI 543 GAGCAC G[AT]GC[AT]^C
AspI 750 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 134 TGCGCA TGC^GCA
BamHI 948 GGATCC G^GATCC
BanII 28 GGGCCC G[GA]GC[CT]^C
BbvCI 276 CCTCAGC CCTCAGC(-5/-2)
BcgI 125 CGAGGGACGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 646 TACGTG [CT]AC^GT[GA]
BsaWI 230 ACCGGA [AT]^CCGG[AT]
BseRI 142 GAGGAG GAGGAG(10/8)
BsiHKAI 543 GAGCAC G[AT]GC[AT]^C
BsmAI 5 GTCTC GTCTC(1/5)
BsmBI 4 CGTCTC CGTCTC(1/5)
BsmI 301 GCATTC GAATGC(1/-1)
BspLU11I 1043 ACATGT A^CATGT
BssHII 490 GCGCGC G^CGCGC
BssSI 638 CTCGTG CACGAG(-5/-1)
DraIII 818 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 515 CGGCCG C^GGCCG
EarI 262 CTCTTC CTCTTC(1/4)
EclXI 515 CGGCCG C^GGCCG
Eco52I 515 CGGCCG C^GGCCG
EcoNI 102 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 134 TGCGCA TGC^GCA
HindIII 1083 AAGCTT A^AGCTT
KpnI 697 GGTACC GGTAC^C
Ksp632I 262 CTCTTC CTCTTC(1/4)
NcoI 49 CCATGG C^CATGG
NotI 514 GCGGCCGC GC^GGCCGC
PflFI 750 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 44 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 388 CTGCAG CTGCA^G
SbfI 387 CCTGCAGG CCTGCA^GG
ScaI 554 AGTACT AGT^ACT
SfaNI 668 GCATC GCATC(5/9)
SfcI 388 CTGCAG C^T[GA][CT]AG
SfiI 285 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 66 CACCGGCG C[GA]^CCGG[CT]G
SmaI 848 CCCGGG CCC^GGG
SmlI 469 CTCAAG C^T[CT][GA]AG
SphI 254 GCATGC GCATG^C
Sse8387I 387 CCTGCAGG CCTGCA^GG
TfiI 970 GAATC G^A[AT]TC
TspRI 996 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 750 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 44 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 848 CCCGGG C^CCGGG
XmaI 848 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 1114 AAATTC [GA]^AATT[CT]
1234 AAATTC
ApoI 1114 AAATTC [GA]^AATT[CT]
1234 AAATTC
AvaII 16 GGTCC G^G[AT]CC
965 GGACC
BbeI 678 GGCGCC GGCGC^C
912 GGCGCC
BglI 166 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
286 GCCGACCTGGC
BlpI 528 GCTGAGC GC^T[ACGT]AGC
540 GCTGAGC
BmyI 28 GGGCCC G[AGT]GC[ACT]^C
543 GAGCAC
BpmI 42 CTCCAG CTGGAG(16/14)
157 CTGGAG
Bpu1102I 528 GCTGAGC GC^T[ACGT]AGC
540 GCTGAGC
BsgI 226 CTGCAC GTGCAG(16/14)
773 GTGCAG
BsiEI 441 CGACCG CG[GA][CT]^CG
515 CGGCCG
Bsp1286I 28 GGGCCC G[AGT]GC[ACT]^C
543 GAGCAC
BspHI 431 TCATGA T^CATGA
506 TCATGA
BsrBI 466 CCGCTC CCGCTC(-3/-3)
495 CCGCTC
BsrFI 13 GCCGGT [GA]^CCGG[CT]
67 ACCGGC
BstYI 857 AGATCC [GA]^GATC[CT]
948 GGATCC
CelII 528 GCTGAGC GC^T[ACGT]AGC
540 GCTGAGC
Cfr10I 13 GCCGGT [GA]^CCGG[CT]
67 ACCGGC
DraII 27 GGGGCCC [GA]G^G[ACGT]CC[CT]
28 GGGCCCT
EcoO109I 27 GGGGCCC [GA]G^G[ACGT]CC[CT]
28 GGGCCCT
EcoT14I 49 CCATGG C^C[AT][AT]GG
593 CCAAGG
KasI 678 GGCGCC G^GCGCC
912 GGCGCC
KspI 351 CCGCGG CCGC^GG
741 CCGCGG
MflI 857 AGATCC [GA]^GATC[CT]
948 GGATCC
MspA1I 351 CCGCGG C[AC]G^C[GT]G
741 CCGCGG
NarI 678 GGCGCC GG^CGCC
912 GGCGCC
PleI 474 GACTC GAGTC(4/5)
1207 GACTC
RcaI 431 TCATGA T^CATGA
506 TCATGA
SacII 351 CCGCGG CCGC^GG
741 CCGCGG
SfoI 678 GGCGCC GGC^GCC
912 GGCGCC
StyI 49 CCATGG C^C[AT][AT]GG
593 CCAAGG
TaqI 575 TCGA T^CGA
1173 TCGA
Tsp509I 1115 AATT ^AATT
1235 AATT
TthHB8I 575 TCGA T^CGA
1173 TCGA
XhoII 857 AGATCC [GA]^GATC[CT]
948 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI BalI BanI BbsI
BbvI BcnI BpuAI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I