Restriction map for
pF1KB8994
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlpI Bpu1102I
BpuAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MslI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 3 GACGCC G[GA]^CG[CT]C
AfaI 495 GTAC GT^AC
Alw44I 313 GTGCAC G^TGCAC
AlwNI 895 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 339 CCTGAGG CC^T[ACGT]AGG
ApaLI 313 GTGCAC G^TGCAC
AspHI 313 GTGCAC G[AT]GC[AT]^C
AviII 134 TGCGCA TGC^GCA
AvrII 798 CCTAGG C^CTAGG
BglI 762 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 798 CCTAGG C^CTAGG
BsaAI 715 TACGTG [CT]AC^GT[GA]
BsaHI 3 GACGCC G[GA]^CG[CT]C
BsgI 812 GTGCAG GTGCAG(16/14)
BsiHKAI 313 GTGCAC G[AT]GC[AT]^C
BsmI 365 GCATTC GAATGC(1/-1)
BsrFI 596 GCCGGC [GA]^CCGG[CT]
Bsu36I 339 CCTGAGG CC^T[ACGT]AGG
Cfr10I 596 GCCGGC [GA]^CCGG[CT]
DpnII 847 GATC ^GATC
DrdI 777 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 339 CCTGAGG CC^T[ACGT]AGG
FspI 134 TGCGCA TGC^GCA
HgaI 3 GACGC GACGC(5/10)
Hin1I 3 GACGCC G[GA]^CG[CT]C
HincII 970 GTCGAC GT[CT]^[GA]AC
HindII 970 GTCGAC GT[CT]^[GA]AC
HinfI 228 GAGTC G^A[ACGT]TC
MaeII 716 ACGT A^CGT
MaeIII 239 GTTAC ^GT[ACGT]AC
MboI 847 GATC ^GATC
NaeI 596 GCCGGC GCC^GGC
NcoI 833 CCATGG C^CATGG
NdeII 847 GATC ^GATC
NgoMIV 596 GCCGGC G^CCGGC
PflMI 142 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 228 GAGTC GAGTC(4/5)
PvuII 580 CAGCTG CAG^CTG
RsaI 495 GTAC GT^AC
SalI 970 GTCGAC G^TCGAC
Sau3AI 847 GATC ^GATC
SbfI 891 CCTGCAGG CCTGCA^GG
SfaNI 113 GCATC GCATC(5/9)
Sse8387I 891 CCTGCAGG CCTGCA^GG
Van91I 142 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 259 GTCTAC GT^[AC][GT]AC
970 GTCGAC
AluI 463 AGCT AG^CT
581 AGCT
Aor51HI 323 AGCGCT AGC^GCT
851 AGCGCT
ApaI 644 GGGCCC GGGCC^C
709 GGGCCC
AvaI 641 CCCGGG C^[CT]CG[GA]G
917 CCCGGG
BalI 563 TGGCCA TGG^CCA
719 TGGCCA
BfaI 103 CTAG C^TAG
799 CTAG
BsoBI 641 CCCGGG C^[CT]CG[GA]G
917 CCCGGG
BspMI 95 ACCTGC ACCTGC(4/8)
144 ACCTGC
BsrBI 422 CCGCTC CCGCTC(-3/-3)
604 GAGCGG
DsaI 527 CCGTGG C^C[GA][CT]GG
833 CCATGG
EaeI 563 TGGCCA [CT]^GGCC[GA]
719 TGGCCA
EarI 691 CTCTTC CTCTTC(1/4)
908 GAAGAG
Eco47III 323 AGCGCT AGC^GCT
851 AGCGCT
HphI 86 TCACC GGTGA(8/7)
386 TCACC
Ksp632I 691 CTCTTC CTCTTC(1/4)
908 GAAGAG
MaeI 103 CTAG C^TAG
799 CTAG
MluNI 563 TGGCCA TGG^CCA
719 TGGCCA
MscI 563 TGGCCA TGG^CCA
719 TGGCCA
MspA1I 580 CAGCTG C[AC]G^C[GT]G
815 CAGCGG
SapI 908 GAAGAGC GCTCTTC(1/4)
690 GCTCTTC
SmaI 641 CCCGGG CCC^GGG
917 CCCGGG
TaqI 500 TCGA T^CGA
971 TCGA
TthHB8I 500 TCGA T^CGA
971 TCGA
XmaCI 641 CCCGGG C^CCGGG
917 CCCGGG
XmaI 641 CCCGGG C^CCGGG
917 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrDI BsrI BssKI BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI StyI TseI TspRI XcmI