Restriction map for
pF1KB8993
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 914 GTAC GT^AC
AflII 504 CTTAAG C^TTAAG
AflIII 6 ACATGT A^C[GA][CT]GT
AhdI 112 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 319 ATTAAT AT^TAAT
AsnI 319 ATTAAT AT^TAAT
AspEI 112 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 838 GGACC G^G[AT]CC
BamHI 160 GGATCC G^GATCC
BfrI 504 CTTAAG C^TTAAG
BglII 454 AGATCT A^GATCT
BsaI 74 GGTCTC GGTCTC(1/5)
BseRI 812 CTCCTC GAGGAG(10/8)
BsiEI 1121 CGATCG CG[GA][CT]^CG
BspLU11I 6 ACATGT A^CATGT
BsrI 1176 ACTGG ACTGG(1/-1)
BstF5I 294 GGATG GGATG(2/0)
DraIII 937 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1102 CCGTGG C^C[GA][CT]GG
Eam1105I 112 GACTTACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 65 CCTCCACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1043 GATATC GAT^ATC
FokI 294 GGATG GGATG(9/13)
HaeIII 934 GGCC GG^CC
HincII 885 GTTGAC GT[CT]^[GA]AC
HindII 885 GTTGAC GT[CT]^[GA]AC
MspA1I 1136 CAGCTG C[AC]G^C[GT]G
NdeI 898 CATATG CA^TATG
NheI 1155 GCTAGC G^CTAGC
PleI 106 GACTC GAGTC(4/5)
PshBI 319 ATTAAT AT^TAAT
PvuI 1121 CGATCG CGAT^CG
PvuII 1136 CAGCTG CAG^CTG
RsaI 914 GTAC GT^AC
SapI 1159 GCTCTTC GCTCTTC(1/4)
SfaNI 385 GATGC GCATC(5/9)
SspI 372 AATATT AAT^ATT
SwaI 731 ATTTAAAT ATTT^AAAT
TaqI 405 TCGA T^CGA
TthHB8I 405 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 161 GATCC GGATC(4/5)
160 GGATC
BbvI 688 GCTGC GCAGC(8/12)
1138 GCTGC
BsiYI 65 CCTCCACTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
95 CCTTCCCTGGG
BslI 65 CCTCCACTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
95 CCTTCCCTGGG
BsmAI 75 GTCTC GTCTC(1/5)
196 GTCTC
BsmFI 104 GGGAC GGGAC(10/14)
790 GTCCC
BstYI 160 GGATCC [GA]^GATC[CT]
454 AGATCT
Cfr13I 838 GGACC G^G[ACGT]CC
933 GGGCC
DraI 732 TTTAAA TTT^AAA
876 TTTAAA
Fnu4HI 688 GCTGC GC^[ACGT]GC
1138 GCTGC
ItaI 688 GCTGC GC^[ACGT]GC
1138 GCTGC
MflI 160 GGATCC [GA]^GATC[CT]
454 AGATCT
Sau96I 838 GGACC G^G[ACGT]CC
933 GGGCC
SmlI 23 CTCAAG C^T[CT][GA]AG
504 CTTAAG
TseI 688 GCTGC G^C[AT]GC
1138 GCTGC
Tsp45I 153 GTCAC ^GT[GC]AC
269 GTGAC
TspRI 1016 CAGTG CA[GC]TG[ACGT][ACGT]^
1175 CACTG
XhoII 160 GGATCC [GA]^GATC[CT]
454 AGATCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AluI ApoI BfaI BsaJI
BssKI BstNI Cac8I DdeI DpnII
EarI EcoRII HinfI HphI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV NspI Sau3AI ScrFI TfiI
Tru9I Tsp509I