Restriction map for
pF1KB8989
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
AspEI AspI AvrII BalI BamHI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BsrI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PstI PvuI RsrII
SacI SalI SbfI ScaI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 873 GACGTC GACGT^C
Acc65I 44 GGTACC G^GTACC
Alw44I 1105 GTGCAC G^TGCAC
ApaLI 1105 GTGCAC G^TGCAC
Asp700I 699 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 44 GGTACC G^GTACC
BanII 83 GGGCTC G[GA]GC[CT]^C
BbrPI 913 CACGTG CAC^GTG
BclI 128 TGATCA T^GATCA
BsaAI 913 CACGTG [CT]AC^GT[GA]
BsgI 256 GTGCAG GTGCAG(16/14)
BsiEI 31 CGGCCG CG[GA][CT]^CG
BspHI 458 TCATGA T^CATGA
BspMI 1079 ACCTGC ACCTGC(4/8)
BssHII 925 GCGCGC G^CGCGC
BssSI 306 CACGAG CACGAG(-5/-1)
BstAPI 1107 GCACGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1013 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 451 TTTAAA TTT^AAA
DraIII 913 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 938 CCGCGG C^C[GA][CT]GG
EagI 31 CGGCCG C^GGCCG
EarI 121 GAAGAG CTCTTC(1/4)
EclXI 31 CGGCCG C^GGCCG
Eco52I 31 CGGCCG C^GGCCG
FbaI 128 TGATCA T^GATCA
KpnI 44 GGTACC GGTAC^C
Ksp632I 121 GAAGAG CTCTTC(1/4)
KspI 938 CCGCGG CCGC^GG
MseI 452 TTAA T^TAA
MslI 1014 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 30 GCGGCCGC GC^GGCCGC
PmaCI 913 CACGTG CAC^GTG
PmlI 913 CACGTG CAC^GTG
PpuMI 5 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 936 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 458 TCATGA T^CATGA
SacII 938 CCGCGG CCGC^GG
SapI 121 GAAGAGC GCTCTTC(1/4)
SexAI 140 ACCAGGT A^CC[AT]GGT
SmaI 40 CCCGGG CCC^GGG
StuI 701 AGGCCT AGG^CCT
TaqI 26 TCGA T^CGA
Tru9I 452 TTAA T^TAA
TspRI 254 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 26 TCGA T^CGA
XmaCI 40 CCCGGG C^CCGGG
XmaI 40 CCCGGG C^CCGGG
XmnI 699 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 193 GTAGAC GT^[AC][GT]AC
943 GTCTAC
AlwI 201 GATCC GGATC(4/5)
981 GATCC
AspHI 1003 GTGCTC G[AT]GC[AT]^C
1105 GTGCAC
AvaI 40 CCCGGG C^[CT]CG[GA]G
574 CCCGAG
AvaII 6 GGACC G^G[AT]CC
138 GGACC
AviII 341 TGCGCA TGC^GCA
890 TGCGCA
BbeI 440 GGCGCC GGCGC^C
1033 GGCGCC
BlpI 162 GCTGAGC GC^T[ACGT]AGC
276 GCTGAGC
Bpu1102I 162 GCTGAGC GC^T[ACGT]AGC
276 GCTGAGC
BsiHKAI 1003 GTGCTC G[AT]GC[AT]^C
1105 GTGCAC
BsmFI 881 GGGAC GGGAC(10/14)
645 GTCCC
BsoBI 40 CCCGGG C^[CT]CG[GA]G
574 CCCGAG
BstF5I 264 CATCC GGATG(2/0)
471 GGATG
BstYI 200 AGATCC [GA]^GATC[CT]
980 AGATCC
CelII 162 GCTGAGC GC^T[ACGT]AGC
276 GCTGAGC
DraII 5 AGGACCC [GA]G^G[ACGT]CC[CT]
59 AGGCCCC
EaeI 31 CGGCCG [CT]^GGCC[GA]
488 CGGCCA
EcoO109I 5 AGGACCC [GA]G^G[ACGT]CC[CT]
59 AGGCCCC
FokI 264 CATCC GGATG(9/13)
471 GGATG
FspI 341 TGCGCA TGC^GCA
890 TGCGCA
HincII 355 GTCAAC GT[CT]^[GA]AC
418 GTCAAC
HindII 355 GTCAAC GT[CT]^[GA]AC
418 GTCAAC
KasI 440 GGCGCC G^GCGCC
1033 GGCGCC
MflI 200 AGATCC [GA]^GATC[CT]
980 AGATCC
NarI 440 GGCGCC GG^CGCC
1033 GGCGCC
NlaIII 459 CATG CATG^
819 CATG
PvuII 205 CAGCTG CAG^CTG
220 CAGCTG
SfaNI 263 GCATC GCATC(5/9)
500 GCATC
SfoI 440 GGCGCC GGC^GCC
1033 GGCGCC
XhoII 200 AGATCC [GA]^GATC[CT]
980 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiYI BslI Bsp1286I BsrBI BsrFI
BssKI BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SmlI StyI
TseI XcmI