Restriction map for
pF1KB8982
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BciVI BclI
BfrI BlnI BpmI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HindIII HinfI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 556 CGCG CG^CG
AcsI 630 AAATTT [GA]^AATT[CT]
AfaI 453 GTAC GT^AC
AlwI 1048 GGATC GGATC(4/5)
AocI 742 CCTCAGG CC^T[ACGT]AGG
ApaI 199 GGGCCC GGGCC^C
ApoI 630 AAATTT [GA]^AATT[CT]
BalI 401 TGGCCA TGG^CCA
BbsI 696 GAAGAC GAAGAC(2/6)
BcgI 1013 CGAACCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 671 CTAG C^TAG
BglI 679 GCCTCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 443 AGATCT A^GATCT
BpuAI 696 GAAGAC GAAGAC(2/6)
BsaAI 454 TACGTG [CT]AC^GT[GA]
BseRI 346 GAGGAG GAGGAG(10/8)
BsgI 271 GTGCAG GTGCAG(16/14)
BsmAI 710 GTCTC GTCTC(1/5)
BssSI 882 CACGAG CACGAG(-5/-1)
BstUI 556 CGCG CG^CG
Bsu36I 742 CCTCAGG CC^T[ACGT]AGG
EarI 648 GAAGAG CTCTTC(1/4)
Eco81I 742 CCTCAGG CC^T[ACGT]AGG
EcoNI 252 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1090 GTCAAC GT[CT]^[GA]AC
HindII 1090 GTCAAC GT[CT]^[GA]AC
Ksp632I 648 GAAGAG CTCTTC(1/4)
MaeI 671 CTAG C^TAG
MaeII 455 ACGT A^CGT
MluNI 401 TGGCCA TGG^CCA
MscI 401 TGGCCA TGG^CCA
MseI 833 TTAA T^TAA
MvnI 556 CGCG CG^CG
NcoI 398 CCATGG C^CATGG
NspI 1031 ACATGC [GA]CATG^[CT]
PstI 39 CTGCAG CTGCA^G
RsaI 453 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
SfaNI 59 GCATC GCATC(5/9)
SfcI 39 CTGCAG C^T[GA][CT]AG
SmaI 570 CCCGGG CCC^GGG
StuI 260 AGGCCT AGG^CCT
Tru9I 833 TTAA T^TAA
XmaCI 570 CCCGGG C^CCGGG
XmaI 570 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 21 GGCGCC G[GA]^CG[CT]C
820 GGCGCC
Asp700I 99 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
612 GAAAATGTTC
AspHI 3 GAGCTC G[AT]GC[AT]^C
1054 GTGCTC
AvaI 76 CCCGAG C^[CT]CG[GA]G
570 CCCGGG
AvaII 590 GGACC G^G[AT]CC
597 GGACC
BbeI 21 GGCGCC GGCGC^C
820 GGCGCC
BbvCI 130 CCTCAGC CCTCAGC(-5/-2)
981 CCTCAGC
BlpI 1035 GCTCAGC GC^T[ACGT]AGC
1056 GCTCAGC
Bpu1102I 1035 GCTCAGC GC^T[ACGT]AGC
1056 GCTCAGC
BsaHI 21 GGCGCC G[GA]^CG[CT]C
820 GGCGCC
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
1054 GTGCTC
BsoBI 76 CCCGAG C^[CT]CG[GA]G
570 CCCGGG
BspMI 14 ACCTGC ACCTGC(4/8)
224 ACCTGC
BsrBI 970 CCGCTC CCGCTC(-3/-3)
867 GAGCGG
BsrFI 18 GCCGGC [GA]^CCGG[CT]
493 GCCGGC
BstXI 258 CCAGGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
990 CCACAGCGGTGG
BstYI 443 AGATCT [GA]^GATC[CT]
1048 GGATCT
CelII 1035 GCTCAGC GC^T[ACGT]AGC
1056 GCTCAGC
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
493 GCCGGC
DpnII 444 GATC ^GATC
1049 GATC
Hin1I 21 GGCGCC G[GA]^CG[CT]C
820 GGCGCC
KasI 21 GGCGCC G^GCGCC
820 GGCGCC
MboI 444 GATC ^GATC
1049 GATC
MflI 443 AGATCT [GA]^GATC[CT]
1048 GGATCT
NaeI 18 GCCGGC GCC^GGC
493 GCCGGC
NarI 21 GGCGCC GG^CGCC
820 GGCGCC
NdeII 444 GATC ^GATC
1049 GATC
NgoMIV 18 GCCGGC G^CCGGC
493 GCCGGC
PflMI 450 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCACCCCTTGG
Sau3AI 444 GATC ^GATC
1049 GATC
SfoI 21 GGCGCC GGC^GCC
820 GGCGCC
Van91I 450 CCAGTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCACCCCTTGG
XhoII 443 AGATCT [GA]^GATC[CT]
1048 GGATCT
XmnI 99 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
612 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI BanI BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I