Restriction map for
pF1KB8978
##################################
# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspEI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstUI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MflI
MluI MroI MspI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
ApaI 122 GGGCCC GGGCC^C
AspHI 86 GTGCTC G[AT]GC[AT]^C
AvaI 666 CTCGGG C^[CT]CG[GA]G
BalI 251 TGGCCA TGG^CCA
BbsI 1100 GTCTTC GAAGAC(2/6)
BclI 485 TGATCA T^GATCA
BpuAI 1100 GTCTTC GAAGAC(2/6)
BsaI 1011 GAGACC GGTCTC(1/5)
BsaJI 519 CCCAGG C^C[ACGT][ACGT]GG
BseRI 1048 CTCCTC GAGGAG(10/8)
BsiHKAI 86 GTGCTC G[AT]GC[AT]^C
BsmFI 517 GTCCC GGGAC(10/14)
BsmI 327 GAATGC GAATGC(1/-1)
BsoBI 666 CTCGGG C^[CT]CG[GA]G
BspDI 406 ATCGAT AT^CGAT
BspHI 743 TCATGA T^CATGA
BsrDI 331 GCAATG GCAATG(2/0)
BssSI 584 CACGAG CACGAG(-5/-1)
BstEII 459 GGTCACC G^GT[ACGT]ACC
BstPI 459 GGTCACC G^GT[ACGT]ACC
ClaI 406 ATCGAT AT^CGAT
DdeI 820 CTGAG C^T[ACGT]AG
DraI 444 TTTAAA TTT^AAA
DraII 121 GGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 251 TGGCCA [CT]^GGCC[GA]
EcoO109I 121 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 459 GGTCACC G^GT[ACGT]ACC
EcoRI 808 GAATTC G^AATTC
FbaI 485 TGATCA T^GATCA
HincII 842 GTCAAC GT[CT]^[GA]AC
HindII 842 GTCAAC GT[CT]^[GA]AC
MaeII 107 ACGT A^CGT
MfeI 1076 CAATTG C^AATTG
MluNI 251 TGGCCA TGG^CCA
MscI 251 TGGCCA TGG^CCA
MspA1I 640 CCGCTG C[AC]G^C[GT]G
MunI 1076 CAATTG C^AATTG
NspI 179 ACATGC [GA]CATG^[CT]
PflMI 988 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 743 TCATGA T^CATGA
SexAI 490 ACCTGGT A^CC[AT]GGT
SfaNI 896 GCATC GCATC(5/9)
SfcI 963 CTGTAG C^T[GA][CT]AG
SspI 778 AATATT AAT^ATT
Van91I 988 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 100 AAATTT [GA]^AATT[CT]
808 GAATTC
AluI 215 AGCT AG^CT
954 AGCT
ApoI 100 AAATTT [GA]^AATT[CT]
808 GAATTC
Asp700I 594 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
795 GAACACCTTC
BsmAI 871 GAGAC GTCTC(1/5)
1011 GAGAC
BspMI 239 ACCTGC ACCTGC(4/8)
861 GCAGGT
BsrBI 139 CCGCTC CCGCTC(-3/-3)
45 GAGCGG
BstXI 583 CCACGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
839 CCAGTCAACTGG
DpnII 262 GATC ^GATC
486 GATC
EarI 760 CTCTTC CTCTTC(1/4)
708 GAAGAG
Ksp632I 760 CTCTTC CTCTTC(1/4)
708 GAAGAG
MboI 262 GATC ^GATC
486 GATC
MseI 199 TTAA T^TAA
445 TTAA
NdeII 262 GATC ^GATC
486 GATC
Sau3AI 262 GATC ^GATC
486 GATC
Tru9I 199 TTAA T^TAA
445 TTAA
XmnI 594 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
795 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AvaII BanII BbvI BmyI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboII MnlI MslI MvaI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I