Restriction map for
pF1KB8976
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MroI MseI MunI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccII 1001 CGCG CG^CG
AcyI 749 GGCGCC G[GA]^CG[CT]C
AfaI 1025 GTAC GT^AC
Alw44I 724 GTGCAC G^TGCAC
AlwI 222 GATCC GGATC(4/5)
ApaI 127 GGGCCC GGGCC^C
ApaLI 724 GTGCAC G^TGCAC
AspHI 724 GTGCAC G[AT]GC[AT]^C
AvaI 31 CTCGAG C^[CT]CG[GA]G
BbeI 749 GGCGCC GGCGC^C
BbsI 301 GTCTTC GAAGAC(2/6)
BbvCI 754 CCTCAGC CCTCAGC(-5/-2)
BfaI 1058 CTAG C^TAG
BpuAI 301 GTCTTC GAAGAC(2/6)
BsaAI 712 TACGTG [CT]AC^GT[GA]
BsaHI 749 GGCGCC G[GA]^CG[CT]C
BsaI 259 GAGACC GGTCTC(1/5)
BseRI 487 CTCCTC GAGGAG(10/8)
BsgI 382 CTGCAC GTGCAG(16/14)
BsiHKAI 724 GTGCAC G[AT]GC[AT]^C
BsmI 1092 GAATGC GAATGC(1/-1)
BsoBI 31 CTCGAG C^[CT]CG[GA]G
BspMI 877 ACCTGC ACCTGC(4/8)
BsrBI 229 GAGCGG CCGCTC(-3/-3)
BsrI 60 ACTGG ACTGG(1/-1)
BstAPI 726 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 815 GGTCACC G^GT[ACGT]ACC
BstPI 815 GGTCACC G^GT[ACGT]ACC
BstUI 1001 CGCG CG^CG
BstXI 515 CCATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 912 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 815 GGTCACC G^GT[ACGT]ACC
EcoT14I 573 CCAAGG C^C[AT][AT]GG
HaeII 749 GGCGCC [GA]GCGC^[CT]
Hin1I 749 GGCGCC G[GA]^CG[CT]C
HincII 391 GTCAAC GT[CT]^[GA]AC
HindII 391 GTCAAC GT[CT]^[GA]AC
KasI 749 GGCGCC G^GCGCC
MaeI 1058 CTAG C^TAG
MvnI 1001 CGCG CG^CG
NaeI 973 GCCGGC GCC^GGC
NarI 749 GGCGCC GG^CGCC
NgoMIV 973 GCCGGC G^CCGGC
NspI 636 GCATGC [GA]CATG^[CT]
PaeR7I 31 CTCGAG C^TCGAG
PleI 1084 GAGTC GAGTC(4/5)
PstI 402 CTGCAG CTGCA^G
RsaI 1025 GTAC GT^AC
SapI 1050 GCTCTTC GCTCTTC(1/4)
SfoI 749 GGCGCC GGC^GCC
SgrAI 972 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 31 CTCGAG C^T[CT][GA]AG
SphI 636 GCATGC GCATG^C
StyI 573 CCAAGG C^C[AT][AT]GG
TaqI 32 TCGA T^CGA
TthHB8I 32 TCGA T^CGA
XhoI 31 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 233 GGCACC G^G[CT][GA]CC
749 GGCGCC
BanII 127 GGGCCC G[GA]GC[CT]^C
330 GGGCTC
BpmI 40 CTGGAG CTGGAG(16/14)
226 CTGGAG
BsmAI 259 GAGAC GTCTC(1/5)
1076 GTCTC
BsmFI 352 GGGAC GGGAC(10/14)
511 GGGAC
BstF5I 495 CATCC GGATG(2/0)
783 CATCC
DdeI 755 CTCAG C^T[ACGT]AG
1082 CTGAG
DpnII 198 GATC ^GATC
222 GATC
EarI 556 CTCTTC CTCTTC(1/4)
1051 CTCTTC
FauI 9 CCCGC CCCGC(4/6)
673 GCGGG
FokI 495 CATCC GGATG(9/13)
783 CATCC
Ksp632I 556 CTCTTC CTCTTC(1/4)
1051 CTCTTC
MboI 198 GATC ^GATC
222 GATC
MspA1I 745 CAGCGG C[AC]G^C[GT]G
757 CAGCGG
NdeII 198 GATC ^GATC
222 GATC
Sau3AI 198 GATC ^GATC
222 GATC
SfaNI 425 GCATC GCATC(5/9)
797 GCATC
SfcI 171 CTACAG C^T[GA][CT]AG
402 CTGCAG
TspRI 940 CACTG CA[GC]TG[ACGT][ACGT]^
990 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AvaII BalI BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrFI BssKI BstNI Cac8I CfoI
Cfr10I Cfr13I DraII DsaI EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NlaIII NlaIV PpuMI Sau96I
ScrFI TfiI TseI Tsp45I XcmI