Restriction map for
pF1KB8974
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HhaI HinP1I KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 214 GTCTAC GT^[AC][GT]AC
AciI 972 CCGC CCGC(-3/-1)
AcsI 264 GAATTT [GA]^AATT[CT]
AcyI 838 GACGCC G[GA]^CG[CT]C
AflIII 251 ACATGT A^C[GA][CT]GT
AlwI 978 GGATC GGATC(4/5)
AocI 1088 CCTCAGG CC^T[ACGT]AGG
ApoI 264 GAATTT [GA]^AATT[CT]
BanI 184 GGTGCC G^G[CT][GA]CC
BclI 407 TGATCA T^GATCA
BcnI 975 CCGGG CC^[GC]GG
BfaI 608 CTAG C^TAG
BpmI 1078 CTGGAG CTGGAG(16/14)
BsaHI 838 GACGCC G[GA]^CG[CT]C
BseRI 283 CTCCTC GAGGAG(10/8)
BspLU11I 251 ACATGT A^CATGT
BstEII 900 GGTCACC G^GT[ACGT]ACC
BstF5I 540 CATCC GGATG(2/0)
BstPI 900 GGTCACC G^GT[ACGT]ACC
BstYI 978 GGATCT [GA]^GATC[CT]
Bsu36I 1088 CCTCAGG CC^T[ACGT]AGG
EarI 299 CTCTTC CTCTTC(1/4)
Eco81I 1088 CCTCAGG CC^T[ACGT]AGG
EcoO65I 900 GGTCACC G^GT[ACGT]ACC
FbaI 407 TGATCA T^GATCA
FokI 540 CATCC GGATG(9/13)
HgaI 838 GACGC GACGC(5/10)
Hin1I 838 GACGCC G[GA]^CG[CT]C
HindIII 1053 AAGCTT A^AGCTT
HpaI 85 GTTAAC GTT^AAC
Ksp632I 299 CTCTTC CTCTTC(1/4)
MaeI 608 CTAG C^TAG
MaeII 83 ACGT A^CGT
MfeI 272 CAATTG C^AATTG
MflI 978 GGATCT [GA]^GATC[CT]
MseI 86 TTAA T^TAA
MunI 272 CAATTG C^AATTG
NciI 975 CCGGG CC^[GC]GG
NlaIV 184 GGTGCC GG[ACGT]^[ACGT]CC
SfcI 390 CTACAG C^T[GA][CT]AG
Tru9I 86 TTAA T^TAA
XhoII 978 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BsiYI 158 CCTTGTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
561 CCAAATCAAGG
BslI 158 CCTTGTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
561 CCAAATCAAGG
BsmAI 1081 GAGAC GTCTC(1/5)
795 GTCTC
Bsp1407I 374 TGTACA T^GTACA
488 TGTACA
BsrGI 374 TGTACA T^GTACA
488 TGTACA
BssSI 169 CTCGTG CACGAG(-5/-1)
982 CTCGTG
Cac8I 139 GCGAGC GC[ACGT]^[ACGT]GC
691 GCTTGC
Cfr13I 311 GGGCC G^G[ACGT]CC
1020 GGCCC
DdeI 797 CTCAG C^T[ACGT]AG
1089 CTCAG
DpnII 408 GATC ^GATC
979 GATC
DsaI 71 CCATGG C^C[GA][CT]GG
596 CCATGG
HapII 578 CCGG C^CGG
975 CCGG
HincII 85 GTTAAC GT[CT]^[GA]AC
364 GTCAAC
HindII 85 GTTAAC GT[CT]^[GA]AC
364 GTCAAC
HpaII 578 CCGG C^CGG
975 CCGG
HphI 961 GGTGA GGTGA(8/7)
902 TCACC
MboI 408 GATC ^GATC
979 GATC
MslI 228 CACAAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
417 CATCAGCGTG
MspI 578 CCGG C^CGG
975 CCGG
NcoI 71 CCATGG C^CATGG
596 CCATGG
NdeII 408 GATC ^GATC
979 GATC
NspI 251 ACATGT [GA]CATG^[CT]
371 GCATGT
Sau3AI 408 GATC ^GATC
979 GATC
Sau96I 311 GGGCC G^G[ACGT]CC
1020 GGCCC
SspBI 374 TGTACA T^GTACA
488 TGTACA
Tsp509I 265 AATT ^AATT
273 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AfaI AluI AspHI BbsI BbvI
BmyI BpuAI BsaJI BsgI BsiHKAI
Bsp1286I BsrDI BsrI BssKI BstNI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
ItaI MaeIII MboII MnlI MspA1I
MvaI MwoI NlaIII PvuII RsaI
ScrFI SfaNI StyI TfiI TseI
Tsp45I TspRI XcmI