Restriction map for
pF1KB8970
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbrPI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BsaAI BsaI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MaeII MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SfiI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AluI 779 AGCT AG^CT
AlwI 783 GGATC GGATC(4/5)
AvaI 129 CTCGGG C^[CT]CG[GA]G
AviII 665 TGCGCA TGC^GCA
AvrII 399 CCTAGG C^CTAGG
BalI 371 TGGCCA TGG^CCA
BbeI 658 GGCGCC GGCGC^C
BbvCI 763 CCTCAGC CCTCAGC(-5/-2)
BfaI 400 CTAG C^TAG
BglI 89 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 979 AGATCT A^GATCT
BlnI 399 CCTAGG C^CTAGG
BsaWI 269 ACCGGA [AT]^CCGG[AT]
BsoBI 129 CTCGGG C^[CT]CG[GA]G
BspMI 101 ACCTGC ACCTGC(4/8)
BssSI 565 CACGAG CACGAG(-5/-1)
BstAPI 539 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 207 GGTCACC G^GT[ACGT]ACC
BstF5I 873 GGATG GGATG(2/0)
BstPI 207 GGTCACC G^GT[ACGT]ACC
BstYI 979 AGATCT [GA]^GATC[CT]
DraII 892 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 374 CCATGG C^C[GA][CT]GG
EaeI 371 TGGCCA [CT]^GGCC[GA]
EcoNI 713 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 892 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 207 GGTCACC G^GT[ACGT]ACC
FokI 873 GGATG GGATG(9/13)
FspI 665 TGCGCA TGC^GCA
KasI 658 GGCGCC G^GCGCC
MaeI 400 CTAG C^TAG
MaeIII 208 GTCAC ^GT[ACGT]AC
MfeI 947 CAATTG C^AATTG
MflI 979 AGATCT [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 869 TTAA T^TAA
MunI 947 CAATTG C^AATTG
NarI 658 GGCGCC GG^CGCC
NcoI 374 CCATGG C^CATGG
NspI 185 ACATGC [GA]CATG^[CT]
PleI 273 GAGTC GAGTC(4/5)
PpuMI 892 AGGACCT [GA]G^G[AT]CC[CT]
PstI 694 CTGCAG CTGCA^G
ScaI 527 AGTACT AGT^ACT
SexAI 541 ACCTGGT A^CC[AT]GGT
SfaNI 681 GATGC GCATC(5/9)
SfcI 694 CTGCAG C^T[GA][CT]AG
SfoI 658 GGCGCC GGC^GCC
SgrAI 685 CACCGGCG C[GA]^CCGG[CT]G
TaqI 355 TCGA T^CGA
TfiI 1066 GAATC G^A[AT]TC
Tru9I 869 TTAA T^TAA
Tsp45I 208 GTCAC ^GT[GC]AC
TthHB8I 355 TCGA T^CGA
XhoII 979 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 152 GGCGTC G[GA]^CG[CT]C
658 GGCGCC
AlwNI 720 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
766 CAGCCCCTG
BanII 346 GGGCTC G[GA]GC[CT]^C
573 GGGCTC
BbsI 910 GAAGAC GAAGAC(2/6)
178 GTCTTC
BpuAI 910 GAAGAC GAAGAC(2/6)
178 GTCTTC
BsaBI 929 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
963 GATTTTTATC
BsaHI 152 GGCGTC G[GA]^CG[CT]C
658 GGCGCC
BsmFI 722 GGGAC GGGAC(10/14)
759 GTCCC
DpnII 784 GATC ^GATC
980 GATC
DraIII 540 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
822 CACTATGTG
EarI 76 CTCTTC CTCTTC(1/4)
817 CTCTTC
HgaI 153 GCGTC GACGC(5/10)
757 GCGTC
Hin1I 152 GGCGTC G[GA]^CG[CT]C
658 GGCGCC
HinfI 273 GAGTC G^A[ACGT]TC
1066 GAATC
Ksp632I 76 CTCTTC CTCTTC(1/4)
817 CTCTTC
MamI 929 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
963 GATTTTTATC
MboI 784 GATC ^GATC
980 GATC
NdeII 784 GATC ^GATC
980 GATC
SapI 75 GCTCTTC GCTCTTC(1/4)
816 GCTCTTC
Sau3AI 784 GATC ^GATC
980 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AspHI AvaII
BanI BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI Bsp1286I BsrFI BsrI BssHII
BssKI BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
StyI TseI Tsp509I TspRI