Restriction map for
pF1KB8969
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 540 GGCGTC G[GA]^CG[CT]C
AflII 934 CTTAAG C^TTAAG
AflIII 450 ACATGT A^C[GA][CT]GT
AlwNI 748 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 827 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 548 GGACC G^G[AT]CC
BamHI 293 GGATCC G^GATCC
BbsI 571 GAAGAC GAAGAC(2/6)
BfrI 934 CTTAAG C^TTAAG
BglII 721 AGATCT A^GATCT
BpuAI 571 GAAGAC GAAGAC(2/6)
BsaHI 540 GGCGTC G[GA]^CG[CT]C
BspLU11I 450 ACATGT A^CATGT
BspMI 61 ACCTGC ACCTGC(4/8)
BsrBI 394 GAGCGG CCGCTC(-3/-3)
BsrDI 656 GCAATG GCAATG(2/0)
BsrFI 77 ACCGGC [GA]^CCGG[CT]
BstXI 209 CCAGTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 43 GCGC GCG^C
Cfr10I 77 ACCGGC [GA]^CCGG[CT]
Cfr13I 548 GGACC G^G[ACGT]CC
DraI 694 TTTAAA TTT^AAA
DraII 547 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 547 AGGACCT [GA]G^G[ACGT]CC[CT]
HapII 78 CCGG C^CGG
HgaI 541 GCGTC GACGC(5/10)
HhaI 43 GCGC GCG^C
Hin1I 540 GGCGTC G[GA]^CG[CT]C
HinP1I 43 GCGC G^CGC
HindIII 949 AAGCTT A^AGCTT
HpaII 78 CCGG C^CGG
HphI 1048 TCACC GGTGA(8/7)
MnlI 546 GAGG CCTC(7/6)
MspA1I 38 CAGCTG C[AC]G^C[GT]G
MspI 78 CCGG C^CGG
PflMI 747 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 547 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 176 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 35 CTGCAG CTGCA^G
PvuII 38 CAGCTG CAG^CTG
Sau96I 548 GGACC G^G[ACGT]CC
SexAI 550 ACCTGGT A^CC[AT]GGT
SfaNI 190 GCATC GCATC(5/9)
SfcI 35 CTGCAG C^T[GA][CT]AG
SmlI 934 CTTAAG C^T[CT][GA]AG
SspI 420 AATATT AAT^ATT
Van91I 747 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 240 CCATCTCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 827 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 297 CCGC CCGC(-3/-1)
396 GCGG
BanII 625 GGGCTC G[GA]GC[CT]^C
786 GAGCCC
BfaI 25 CTAG C^TAG
373 CTAG
BspHI 107 TCATGA T^CATGA
152 TCATGA
BstF5I 443 CATCC GGATG(2/0)
510 CATCC
Cac8I 951 GCTTGC GC[ACGT]^[ACGT]GC
1069 GCAAGC
FokI 443 CATCC GGATG(9/13)
510 CATCC
MaeI 25 CTAG C^TAG
373 CTAG
NspI 450 ACATGT [GA]CATG^[CT]
975 GCATGT
PleI 407 GAGTC GAGTC(4/5)
1045 GAGTC
RcaI 107 TCATGA T^CATGA
152 TCATGA
TaqI 522 TCGA T^CGA
544 TCGA
Tsp45I 409 GTCAC ^GT[GC]AC
1047 GTCAC
TthHB8I 522 TCGA T^CGA
544 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AluI AlwI ApoI BalI
BbvI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
BstYI DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI HaeIII HinfI ItaI
MaeIII MboI MboII MflI MluNI
MscI MseI MslI MvaI NdeII
NlaIII NlaIV Sau3AI ScrFI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII