Restriction map for
pF1KB8965
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI MaeI
MamI MfeI MluI MroI MseI
MunI NdeI NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp509I TspRI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 709 GTCTAC GT^[AC][GT]AC
AhdI 4 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 714 CTCGGG C^[CT]CG[GA]G
BalI 101 TGGCCA TGG^CCA
BcgI 358 CGAAGGAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 143 AGATCT A^GATCT
BlpI 135 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 135 GCTGAGC GC^T[ACGT]AGC
BseRI 322 GAGGAG GAGGAG(10/8)
BsgI 865 CTGCAC GTGCAG(16/14)
BsmFI 795 GTCCC GGGAC(10/14)
BsoBI 714 CTCGGG C^[CT]CG[GA]G
Bsp1407I 386 TGTACA T^GTACA
BsrGI 386 TGTACA T^GTACA
BssSI 462 CTCGTG CACGAG(-5/-1)
BstEII 1029 GGTCACC G^GT[ACGT]ACC
BstPI 1029 GGTCACC G^GT[ACGT]ACC
BstXI 473 CCAACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 143 AGATCT [GA]^GATC[CT]
CelII 135 GCTGAGC GC^T[ACGT]AGC
DdeI 136 CTGAG C^T[ACGT]AG
DpnII 144 GATC ^GATC
DraII 257 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 556 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 984 CTCTTC CTCTTC(1/4)
EcoO109I 257 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1029 GGTCACC G^GT[ACGT]ACC
Ksp632I 984 CTCTTC CTCTTC(1/4)
MaeII 530 ACGT A^CGT
MboI 144 GATC ^GATC
MflI 143 AGATCT [GA]^GATC[CT]
MluNI 101 TGGCCA TGG^CCA
MscI 101 TGGCCA TGG^CCA
MslI 93 CATCGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 974 CCATGG C^CATGG
NdeII 144 GATC ^GATC
NotI 342 GCGGCCGC GC^GGCCGC
NspI 935 GCATGT [GA]CATG^[CT]
PstI 157 CTGCAG CTGCA^G
Sau3AI 144 GATC ^GATC
SfcI 157 CTGCAG C^T[GA][CT]AG
SmlI 376 CTCAAG C^T[CT][GA]AG
SspBI 386 TGTACA T^GTACA
XcmI 839 CCACGCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 143 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwNI 151 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
787 CAGCCCCTG
AvaII 3 GGACC G^G[AT]CC
290 GGACC
BbsI 36 GAAGAC GAAGAC(2/6)
958 GTCTTC
BpmI 487 CTGGAG CTGGAG(16/14)
850 CTGGAG
BpuAI 36 GAAGAC GAAGAC(2/6)
958 GTCTTC
BspMI 569 ACCTGC ACCTGC(4/8)
1026 GCAGGT
BsrI 287 ACTGG ACTGG(1/-1)
150 CCAGT
HphI 115 TCACC GGTGA(8/7)
1031 TCACC
SfaNI 50 GCATC GCATC(5/9)
905 GCATC
SgrAI 675 CACCGGCG C[GA]^CCGG[CT]G
681 CGCCGGTG
TaqI 296 TCGA T^CGA
320 TCGA
Tsp45I 941 GTGAC ^GT[GC]AC
1030 GTCAC
TthHB8I 296 TCGA T^CGA
320 TCGA
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BanII BbeI BbvI BcnI
BmyI BsaHI BsaJI BsiEI BsiYI
BslI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DsaI EaeI EagI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
KasI KspI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIII
NlaIV RsaI SacII Sau96I ScrFI
SfoI StyI TseI