Restriction map for
pF1KB8964
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MflI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I XbaI XhoI
XhoII
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# ONE CUT ENZYMES ( 66 enzymes) #
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AcyI 396 GGCGCC G[GA]^CG[CT]C
AfaI 273 GTAC GT^AC
AflIII 596 ACATGT A^C[GA][CT]GT
Alw44I 298 GTGCAC G^TGCAC
AlwI 437 GGATC GGATC(4/5)
Aor51HI 476 AGCGCT AGC^GCT
ApaLI 298 GTGCAC G^TGCAC
Asp700I 630 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 699 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 282 CCCGGG C^[CT]CG[GA]G
BalI 383 TGGCCA TGG^CCA
BbeI 396 GGCGCC GGCGC^C
BsaHI 396 GGCGCC G[GA]^CG[CT]C
BseRI 646 GAGGAG GAGGAG(10/8)
BsmFI 697 GGGAC GGGAC(10/14)
BsoBI 282 CCCGGG C^[CT]CG[GA]G
BspLU11I 596 ACATGT A^CATGT
BsrBI 1029 CCGCTC CCGCTC(-3/-3)
BsrFI 78 GCCGGT [GA]^CCGG[CT]
BstEII 81 GGTGACC G^GT[ACGT]ACC
BstPI 81 GGTGACC G^GT[ACGT]ACC
Cfr10I 78 GCCGGT [GA]^CCGG[CT]
DpnII 438 GATC ^GATC
DrdI 517 GACTGCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 448 CTCTTC CTCTTC(1/4)
Eco47III 476 AGCGCT AGC^GCT
EcoO65I 81 GGTGACC G^GT[ACGT]ACC
EcoT22I 1043 ATGCAT ATGCA^T
HgaI 883 GACGC GACGC(5/10)
Hin1I 396 GGCGCC G[GA]^CG[CT]C
KasI 396 GGCGCC G^GCGCC
Ksp632I 448 CTCTTC CTCTTC(1/4)
KspI 728 CCGCGG CCGC^GG
MaeII 962 ACGT A^CGT
MboI 438 GATC ^GATC
MluNI 383 TGGCCA TGG^CCA
MscI 383 TGGCCA TGG^CCA
MseI 1040 TTAA T^TAA
MslI 375 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 396 GGCGCC GG^CGCC
NdeII 438 GATC ^GATC
NsiI 1043 ATGCAT ATGCA^T
NspI 596 ACATGT [GA]CATG^[CT]
PflFI 699 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 27 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1004 GAGTC GAGTC(4/5)
PstI 209 CTGCAG CTGCA^G
RsaI 273 GTAC GT^AC
SacI 859 GAGCTC GAGCT^C
SacII 728 CCGCGG CCGC^GG
Sau3AI 438 GATC ^GATC
SfcI 209 CTGCAG C^T[GA][CT]AG
SfoI 396 GGCGCC GGC^GCC
SgrAI 77 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 282 CCCGGG CCC^GGG
SmlI 417 CTTGAG C^T[CT][GA]AG
StuI 237 AGGCCT AGG^CCT
TaqI 715 TCGA T^CGA
Tru9I 1040 TTAA T^TAA
Tth111I 699 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 715 TCGA T^CGA
Van91I 27 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 953 CCAAACTGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 282 CCCGGG C^CCGGG
XmaI 282 CCCGGG C^CCGGG
XmnI 630 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 70 GTCTAC GT^[AC][GT]AC
1006 GTCTAC
AocI 182 CCTCAGG CC^T[ACGT]AGG
778 CCTGAGG
AspHI 298 GTGCAC G[AT]GC[AT]^C
859 GAGCTC
BanI 195 GGCACC G^G[CT][GA]CC
396 GGCGCC
BsiHKAI 298 GTGCAC G[AT]GC[AT]^C
859 GAGCTC
BspMI 207 ACCTGC ACCTGC(4/8)
617 ACCTGC
BstF5I 387 CATCC GGATG(2/0)
1046 CATCC
Bsu36I 182 CCTCAGG CC^T[ACGT]AGG
778 CCTGAGG
Eco81I 182 CCTCAGG CC^T[ACGT]AGG
778 CCTGAGG
EcoT14I 98 CCATGG C^C[AT][AT]GG
380 CCATGG
FokI 387 CATCC GGATG(9/13)
1046 CATCC
HaeII 396 GGCGCC [GA]GCGC^[CT]
476 AGCGCT
MaeIII 82 GTGAC ^GT[ACGT]AC
739 GTCAC
NcoI 98 CCATGG C^CATGG
380 CCATGG
PpuMI 186 AGGACCC [GA]G^G[AT]CC[CT]
285 GGGTCCT
StyI 98 CCATGG C^C[AT][AT]GG
380 CCATGG
TfiI 129 GAATC G^A[AT]TC
468 GAATC
Tsp45I 82 GTGAC ^GT[GC]AC
739 GTCAC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SfaNI TseI TspRI