Restriction map for
pF1KB8962
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaII AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BsgI BsiEI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrFI Bst1107I BstAPI BstEII
BstF5I BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DsaI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI KasI
KspI MaeII MamI MluI MluNI
MroI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 426 GGTACC G^GTACC
AcsI 856 GAATTT [GA]^AATT[CT]
AcyI 480 GACGCC G[GA]^CG[CT]C
Aor51HI 68 AGCGCT AGC^GCT
ApaI 663 GGGCCC GGGCC^C
ApoI 856 GAATTT [GA]^AATT[CT]
Asp718I 426 GGTACC G^GTACC
AspHI 418 GAGCAC G[AT]GC[AT]^C
BamHI 992 GGATCC G^GATCC
BbvCI 568 CCTCAGC CCTCAGC(-5/-2)
BfaI 308 CTAG C^TAG
BglI 656 GCCTGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 480 GACGCC G[GA]^CG[CT]C
BsaI 598 GAGACC GGTCTC(1/5)
BsiHKAI 418 GAGCAC G[AT]GC[AT]^C
BsiWI 32 CGTACG C^GTACG
BsmFI 167 GGGAC GGGAC(10/14)
Bsp1407I 338 TGTACA T^GTACA
BsrBI 798 GAGCGG CCGCTC(-3/-3)
BsrDI 14 GCAATG GCAATG(2/0)
BsrGI 338 TGTACA T^GTACA
BsrI 533 CCAGT ACTGG(1/-1)
BssHII 546 GCGCGC G^CGCGC
BssSI 421 CACGAG CACGAG(-5/-1)
BstBI 508 TTCGAA TT^CGAA
BstXI 486 CCACTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 992 GGATCC [GA]^GATC[CT]
DpnII 993 GATC ^GATC
DraII 663 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 305 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 810 GAAGAG CTCTTC(1/4)
Eco47III 68 AGCGCT AGC^GCT
EcoO109I 663 GGGCCCT [GA]G^G[ACGT]CC[CT]
Hin1I 480 GACGCC G[GA]^CG[CT]C
KpnI 426 GGTACC GGTAC^C
Ksp632I 810 GAAGAG CTCTTC(1/4)
MaeI 308 CTAG C^TAG
MboI 993 GATC ^GATC
MfeI 117 CAATTG C^AATTG
MflI 992 GGATCC [GA]^GATC[CT]
MslI 90 CATGTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 117 CAATTG C^AATTG
NdeII 993 GATC ^GATC
NspV 508 TTCGAA TT^CGAA
PflMI 834 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI 993 GATC ^GATC
SfcI 72 CTATAG C^T[GA][CT]AG
SfuI 508 TTCGAA TT^CGAA
SmlI 967 CTCAAG C^T[CT][GA]AG
SspBI 338 TGTACA T^GTACA
Tsp45I 110 GTGAC ^GT[GC]AC
TspRI 487 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 834 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 992 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 993 GATCC GGATC(4/5)
992 GGATC
AlwNI 652 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
835 CAGACGCTG
AvaI 370 CCCGAG C^[CT]CG[GA]G
456 CCCGAG
BanI 426 GGTACC G^G[CT][GA]CC
815 GGTGCC
BcnI 55 CCCGG CC^[GC]GG
996 CCGGG
BpmI 717 CTCCAG CTGGAG(16/14)
895 CTGGAG
BsmAI 598 GAGAC GTCTC(1/5)
536 GTCTC
BsoBI 370 CCCGAG C^[CT]CG[GA]G
456 CCCGAG
DdeI 569 CTCAG C^T[ACGT]AG
596 CTGAG
DrdI 600 GACCCCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
925 GACAGACAAGTC
EcoT14I 44 CCTTGG C^C[AT][AT]GG
411 CCTTGG
HaeII 68 AGCGCT [GA]GCGC^[CT]
887 AGCGCC
HphI 126 GGTGA GGTGA(8/7)
1022 TCACC
MaeIII 19 GTAAC ^GT[ACGT]AC
110 GTGAC
MboII 807 GAAGA GAAGA(8/7)
810 GAAGA
MseI 914 TTAA T^TAA
1016 TTAA
NciI 55 CCCGG CC^[GC]GG
996 CCGGG
PvuII 223 CAGCTG CAG^CTG
571 CAGCTG
StyI 44 CCTTGG C^C[AT][AT]GG
411 CCTTGG
TaqI 509 TCGA T^CGA
521 TCGA
TfiI 562 GAATC G^A[AT]TC
987 GAATC
Tru9I 914 TTAA T^TAA
1016 TTAA
TthHB8I 509 TCGA T^CGA
521 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AfaI AluI BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIII NlaIV NspI PleI RsaI
Sau96I ScrFI TseI Tsp509I