Restriction map for
pF1KB8960
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BbeI
BbrPI BbsI BcgI BciVI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsiHKAI BsiWI
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DsaI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI Hin1I HindIII KasI KpnI
KspI MamI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspI StuI StyI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 232 GTAGAC GT^[AC][GT]AC
AocI 430 CCTGAGG CC^T[ACGT]AGG
AspI 458 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 655 CCCGAG C^[CT]CG[GA]G
BalI 170 TGGCCA TGG^CCA
BamHI 986 GGATCC G^GATCC
BanI 809 GGTGCC G^G[CT][GA]CC
BbvCI 697 GCTGAGG CCTCAGC(-5/-2)
BclI 53 TGATCA T^GATCA
BcnI 479 CCGGG CC^[GC]GG
BglI 506 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 256 AGATCT A^GATCT
BpmI 532 CTCCAG CTGGAG(16/14)
BsaI 702 GGTCTC GGTCTC(1/5)
BsaWI 983 ACCGGA [AT]^CCGG[AT]
BseRI 684 GAGGAG GAGGAG(10/8)
BsgI 724 GTGCAG GTGCAG(16/14)
BsiEI 374 CGGCCG CG[GA][CT]^CG
BsmBI 164 CGTCTC CGTCTC(1/5)
BsmI 140 GAATGC GAATGC(1/-1)
BsoBI 655 CCCGAG C^[CT]CG[GA]G
Bsp1407I 101 TGTACA T^GTACA
BspMI 924 GCAGGT ACCTGC(4/8)
BsrBI 615 GAGCGG CCGCTC(-3/-3)
BsrGI 101 TGTACA T^GTACA
BsrI 713 CCAGT ACTGG(1/-1)
BssSI 545 CTCGTG CACGAG(-5/-1)
BstF5I 214 CATCC GGATG(2/0)
Bsu36I 430 CCTGAGG CC^T[ACGT]AGG
DrdI 919 GACAGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 374 CGGCCG C^GGCCG
EclXI 374 CGGCCG C^GGCCG
Eco52I 374 CGGCCG C^GGCCG
Eco81I 430 CCTGAGG CC^T[ACGT]AGG
EcoNI 693 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 875 CCCGC CCCGC(4/6)
FbaI 53 TGATCA T^GATCA
FokI 214 CATCC GGATG(9/13)
HincII 907 GTTAAC GT[CT]^[GA]AC
HindII 907 GTTAAC GT[CT]^[GA]AC
HpaI 907 GTTAAC GTT^AAC
MaeII 1012 ACGT A^CGT
MluNI 170 TGGCCA TGG^CCA
MscI 170 TGGCCA TGG^CCA
NciI 479 CCGGG CC^[GC]GG
PflFI 458 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 580 CAGCTG CAG^CTG
SbfI 62 CCTGCAGG CCTGCA^GG
SfaNI 213 GCATC GCATC(5/9)
Sse8387I 62 CCTGCAGG CCTGCA^GG
SspBI 101 TGTACA T^GTACA
TaqI 264 TCGA T^CGA
Tsp45I 71 GTGAC ^GT[GC]AC
Tth111I 458 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 264 TCGA T^CGA
XcmI 766 CCAACCAGCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 552 CGCG CG^CG
877 CGCG
AlwNI 57 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
394 CAGAGGCTG
BfaI 116 CTAG C^TAG
889 CTAG
BstUI 552 CGCG CG^CG
877 CGCG
DraII 130 GGGACCT [GA]G^G[ACGT]CC[CT]
380 AGGTCCC
EarI 679 GAAGAG CTCTTC(1/4)
804 GAAGAG
EcoO109I 130 GGGACCT [GA]G^G[ACGT]CC[CT]
380 AGGTCCC
HaeII 91 AGCGCC [GA]GCGC^[CT]
881 AGCGCC
HapII 479 CCGG C^CGG
984 CCGG
HpaII 479 CCGG C^CGG
984 CCGG
Ksp632I 679 GAAGAG CTCTTC(1/4)
804 GAAGAG
MaeI 116 CTAG C^TAG
889 CTAG
MaeIII 71 GTGAC ^GT[ACGT]AC
280 GTTAC
MfeI 333 CAATTG C^AATTG
774 CAATTG
MseI 320 TTAA T^TAA
908 TTAA
MslI 99 CATGTACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
522 CACTGTCATG
MspA1I 572 CCGCTG C[AC]G^C[GT]G
580 CAGCTG
MspI 479 CCGG C^CGG
984 CCGG
MunI 333 CAATTG C^AATTG
774 CAATTG
MvnI 552 CGCG CG^CG
877 CGCG
PleI 409 GAGTC GAGTC(4/5)
462 GAGTC
PpuMI 130 GGGACCT [GA]G^G[AT]CC[CT]
380 AGGTCCC
PstI 63 CTGCAG CTGCA^G
583 CTGCAG
SfcI 63 CTGCAG C^T[GA][CT]AG
583 CTGCAG
SmlI 227 CTCAAG C^T[CT][GA]AG
961 CTCAAG
TfiI 46 GATTC G^A[AT]TC
328 GAATC
Tru9I 320 TTAA T^TAA
908 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI RsaI Sau3AI Sau96I
ScrFI TseI Tsp509I TspRI Van91I
XhoII