Restriction map for
pF1KB8958
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BsaBI BsaHI
BsaWI BseAI BseRI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
Hin1I HindIII HpaI KasI KspI
MaeIII MamI MfeI MluNI MroI
MscI MunI NarI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tsp509I Tth111I TthHB8I
XbaI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 365 GGTACC G^GTACC
AflIII 332 ACGCGT A^C[GA][CT]GT
AlwNI 505 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 365 GGTACC G^GTACC
AspHI 499 GAGCAC G[AT]GC[AT]^C
AviII 695 TGCGCA TGC^GCA
BanI 365 GGTACC G^G[CT][GA]CC
BanII 462 GGGCTC G[GA]GC[CT]^C
BbsI 972 GTCTTC GAAGAC(2/6)
BfaI 160 CTAG C^TAG
BglII 821 AGATCT A^GATCT
BlpI 68 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 68 GCTCAGC GC^T[ACGT]AGC
BpuAI 972 GTCTTC GAAGAC(2/6)
BsaAI 522 TACGTA [CT]AC^GT[GA]
BsgI 175 GTGCAG GTGCAG(16/14)
BsiEI 150 CGACCG CG[GA][CT]^CG
BsiHKAI 499 GAGCAC G[AT]GC[AT]^C
BspMI 64 ACCTGC ACCTGC(4/8)
BsrFI 228 GCCGGC [GA]^CCGG[CT]
BssHII 765 GCGCGC G^CGCGC
BstXI 483 CCACTCATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 821 AGATCT [GA]^GATC[CT]
CelII 68 GCTCAGC GC^T[ACGT]AGC
Cfr10I 228 GCCGGC [GA]^CCGG[CT]
DraII 61 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 413 CCATGG C^C[GA][CT]GG
EaeI 890 CGGCCA [CT]^GGCC[GA]
EarI 945 CTCTTC CTCTTC(1/4)
EcoO109I 61 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 413 CCATGG C^C[AT][AT]GG
FspI 695 TGCGCA TGC^GCA
HaeII 127 AGCGCC [GA]GCGC^[CT]
HgaI 762 GACGC GACGC(5/10)
HincII 680 GTCAAC GT[CT]^[GA]AC
HindII 680 GTCAAC GT[CT]^[GA]AC
KpnI 365 GGTACC GGTAC^C
Ksp632I 945 CTCTTC CTCTTC(1/4)
MaeI 160 CTAG C^TAG
MflI 821 AGATCT [GA]^GATC[CT]
MluI 332 ACGCGT A^CGCGT
MseI 337 TTAA T^TAA
MslI 484 CACTCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 383 CCGCTG C[AC]G^C[GT]G
NaeI 228 GCCGGC GCC^GGC
NcoI 413 CCATGG C^CATGG
NdeI 488 CATATG CA^TATG
NgoMIV 228 GCCGGC G^CCGGC
NlaIII 414 CATG CATG^
PleI 55 GACTC GAGTC(4/5)
PpuMI 61 GGGACCT [GA]G^G[AT]CC[CT]
PstI 796 CTGCAG CTGCA^G
SapI 944 GCTCTTC GCTCTTC(1/4)
SfcI 796 CTGCAG C^T[GA][CT]AG
SmaI 966 CCCGGG CCC^GGG
SmlI 276 CTCAAG C^T[CT][GA]AG
SnaBI 522 TACGTA TAC^GTA
StyI 413 CCATGG C^C[AT][AT]GG
TfiI 925 GAATC G^A[AT]TC
Tru9I 337 TTAA T^TAA
XhoII 821 AGATCT [GA]^GATC[CT]
XmaCI 966 CCCGGG C^CCGGG
XmaI 966 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Asp700I 18 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
735 GAAGGAGTTC
BmyI 462 GGGCTC G[AGT]GC[ACT]^C
499 GAGCAC
BpmI 599 CTCCAG CTGGAG(16/14)
730 CTGGAG
BsaI 808 GAGACC GGTCTC(1/5)
871 GGTCTC
BsmFI 61 GGGAC GGGAC(10/14)
540 GTCCC
Bsp1286I 462 GGGCTC G[AGT]GC[ACT]^C
499 GAGCAC
BstF5I 791 CATCC GGATG(2/0)
620 GGATG
FokI 791 CATCC GGATG(9/13)
620 GGATG
HinfI 55 GACTC G^A[ACGT]TC
925 GAATC
PflMI 289 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
504 CCAGAGCCTGG
Van91I 289 CCAAGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
504 CCAGAGCCTGG
XcmI 273 CCACTCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
438 CCAGGGTTATGCTGG
XmnI 18 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
735 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BbvI BcnI BsaJI BsiYI
BslI BsmAI BsoBI BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI