Restriction map for
pF1KB8953
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AviII AvrII BamHI BbrPI
BciVI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsgI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI HphI KpnI MaeIII MamI
MfeI MflI MluI MroI MunI
NcoI NdeI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 72 enzymes) #
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AcsI 702 GAATTT [GA]^AATT[CT]
AflIII 20 ACGTGT A^C[GA][CT]GT
AhdI 379 GACGGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 252 GTGCAC G^TGCAC
AlwI 693 GATCC GGATC(4/5)
ApaLI 252 GTGCAC G^TGCAC
ApoI 702 GAATTT [GA]^AATT[CT]
Asp700I 868 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 379 GACGGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 329 GGTCC G^G[AT]CC
BalI 878 TGGCCA TGG^CCA
BbeI 142 GGCGCC GGCGC^C
BbsI 415 GTCTTC GAAGAC(2/6)
BbvCI 441 CCTCAGC CCTCAGC(-5/-2)
BcgI 273 CGAACCTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 677 CTAG C^TAG
BglI 403 GCCACCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 60 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 60 GCTCAGC GC^T[ACGT]AGC
BpuAI 415 GTCTTC GAAGAC(2/6)
BsaAI 19 TACGTG [CT]AC^GT[GA]
BsaI 386 GGTCTC GGTCTC(1/5)
BseRI 439 CTCCTC GAGGAG(10/8)
BsiWI 341 CGTACG C^GTACG
BsmBI 573 CGTCTC CGTCTC(1/5)
BsmFI 969 GTCCC GGGAC(10/14)
BsmI 636 GAATGC GAATGC(1/-1)
BspMI 288 ACCTGC ACCTGC(4/8)
BsrBI 84 CCGCTC CCGCTC(-3/-3)
BsrFI 336 GCCGGC [GA]^CCGG[CT]
BstXI 887 CCATTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 60 GCTCAGC GC^T[ACGT]AGC
Cfr10I 336 GCCGGC [GA]^CCGG[CT]
DpnII 693 GATC ^GATC
DraII 265 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 808 GACACGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 379 GACGGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 630 GAAGAG CTCTTC(1/4)
EcoO109I 265 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 142 GGCGCC [GA]GCGC^[CT]
KasI 142 GGCGCC G^GCGCC
Ksp632I 630 GAAGAG CTCTTC(1/4)
KspI 293 CCGCGG CCGC^GG
MaeI 677 CTAG C^TAG
MboI 693 GATC ^GATC
MluNI 878 TGGCCA TGG^CCA
MscI 878 TGGCCA TGG^CCA
MseI 760 TTAA T^TAA
NaeI 336 GCCGGC GCC^GGC
NarI 142 GGCGCC GG^CGCC
NdeII 693 GATC ^GATC
NgoMIV 336 GCCGGC G^CCGGC
NheI 676 GCTAGC G^CTAGC
NlaIII 26 CATG CATG^
PaeR7I 764 CTCGAG C^TCGAG
PstI 109 CTGCAG CTGCA^G
SacI 3 GAGCTC GAGCT^C
SacII 293 CCGCGG CCGC^GG
Sau3AI 693 GATC ^GATC
ScaI 755 AGTACT AGT^ACT
SexAI 276 ACCTGGT A^CC[AT]GGT
SfcI 109 CTGCAG C^T[GA][CT]AG
SfoI 142 GGCGCC GGC^GCC
SmaI 351 CCCGGG CCC^GGG
SmlI 764 CTCGAG C^T[CT][GA]AG
Tru9I 760 TTAA T^TAA
Tsp509I 703 AATT ^AATT
TspRI 250 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 764 CTCGAG C^TCGAG
XmaCI 351 CCCGGG C^CCGGG
XmaI 351 CCCGGG C^CCGGG
XmnI 868 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AcyI 91 GACGCC G[GA]^CG[CT]C
142 GGCGCC
BanII 3 GAGCTC G[GA]GC[CT]^C
767 GAGCCC
BsaHI 91 GACGCC G[GA]^CG[CT]C
142 GGCGCC
BsrI 729 ACTGG ACTGG(1/-1)
240 CCAGT
DsaI 101 CCGTGG C^C[GA][CT]GG
293 CCGCGG
HgaI 91 GACGC GACGC(5/10)
505 GCGTC
Hin1I 91 GACGCC G[GA]^CG[CT]C
142 GGCGCC
PvuII 153 CAGCTG CAG^CTG
299 CAGCTG
SfaNI 541 GCATC GCATC(5/9)
776 GCATC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI BanI BbvI BcnI BmyI
BpmI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV RsaI Sau96I ScrFI
TaqI TseI TthHB8I