Restriction map for
pF1KB8951
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BciVI BfaI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MamI MluI MroI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI TfiI TspRI
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 541 GTCTAC GT^[AC][GT]AC
AccII 886 CGCG CG^CG
AflII 898 CTTAAG C^TTAAG
AlwNI 112 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 73 CCCGGG C^[CT]CG[GA]G
BalI 377 TGGCCA TGG^CCA
BamHI 395 GGATCC G^GATCC
BanI 175 GGTGCC G^G[CT][GA]CC
BbsI 607 GTCTTC GAAGAC(2/6)
BbvCI 732 CCTCAGC CCTCAGC(-5/-2)
BclI 497 TGATCA T^GATCA
BfrI 898 CTTAAG C^TTAAG
BglI 289 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 601 CTGGAG CTGGAG(16/14)
BpuAI 607 GTCTTC GAAGAC(2/6)
BsaI 63 GGTCTC GGTCTC(1/5)
BsgI 932 CTGCAC GTGCAG(16/14)
BsmAI 64 GTCTC GTCTC(1/5)
BsmFI 414 GGGAC GGGAC(10/14)
BsoBI 73 CCCGGG C^[CT]CG[GA]G
BspMI 235 ACCTGC ACCTGC(4/8)
BsrBI 457 GAGCGG CCGCTC(-3/-3)
BsrDI 813 CATTGC GCAATG(2/0)
BsrFI 385 GCCGGC [GA]^CCGG[CT]
BstEII 354 GGTGACC G^GT[ACGT]ACC
BstPI 354 GGTGACC G^GT[ACGT]ACC
BstUI 886 CGCG CG^CG
BstXI 698 CCAAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 115 GCGC GCG^C
Cfr10I 385 GCCGGC [GA]^CCGG[CT]
DsaI 170 CCGTGG C^C[GA][CT]GG
EcoO65I 354 GGTGACC G^GT[ACGT]ACC
FbaI 497 TGATCA T^GATCA
HaeII 114 GGCGCT [GA]GCGC^[CT]
HgaI 887 GCGTC GACGC(5/10)
HhaI 115 GCGC GCG^C
HinP1I 115 GCGC G^CGC
MaeII 80 ACGT A^CGT
MfeI 299 CAATTG C^AATTG
MluNI 377 TGGCCA TGG^CCA
MscI 377 TGGCCA TGG^CCA
MseI 899 TTAA T^TAA
MunI 299 CAATTG C^AATTG
MvnI 886 CGCG CG^CG
NaeI 385 GCCGGC GCC^GGC
NgoMIV 385 GCCGGC G^CCGGC
PvuII 735 CAGCTG CAG^CTG
SacI 276 GAGCTC GAGCT^C
ScaI 671 AGTACT AGT^ACT
SmaI 73 CCCGGG CCC^GGG
SmlI 898 CTTAAG C^T[CT][GA]AG
StuI 967 AGGCCT AGG^CCT
Tru9I 899 TTAA T^TAA
Tsp509I 300 AATT ^AATT
XcmI 473 CCAACGGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 73 CCCGGG C^CCGGG
XmaI 73 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AspHI 49 GTGCTC G[AT]GC[AT]^C
276 GAGCTC
BanII 276 GAGCTC G[GA]GC[CT]^C
490 GAGCCC
BsaWI 255 TCCGGT [AT]^CCGG[AT]
338 TCCGGT
BseRI 580 CTCCTC GAGGAG(10/8)
651 CTCCTC
BsiHKAI 49 GTGCTC G[AT]GC[AT]^C
276 GAGCTC
MaeIII 355 GTGAC ^GT[ACGT]AC
889 GTCAC
MslI 42 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
522 CATCAGCATG
MspA1I 735 CAGCTG C[AC]G^C[GT]G
921 CCGCTG
TaqI 44 TCGA T^CGA
512 TCGA
Tsp45I 355 GTGAC ^GT[GC]AC
889 GTCAC
TthHB8I 44 TCGA T^CGA
512 TCGA
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TseI XhoII