Restriction map for
pF1KB8947
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbeI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 780 GGCGTC G[GA]^CG[CT]C
AfaI 438 GTAC GT^AC
AlwNI 796 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 811 GGCACC G^G[CT][GA]CC
BbrPI 171 CACGTG CAC^GTG
BfaI 13 CTAG C^TAG
BlpI 510 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 510 GCTCAGC GC^T[ACGT]AGC
BsaHI 780 GGCGTC G[GA]^CG[CT]C
BsaI 355 GAGACC GGTCTC(1/5)
BsgI 475 GTGCAG GTGCAG(16/14)
BsmFI 680 GGGAC GGGAC(10/14)
BspHI 287 TCATGA T^CATGA
BsrI 627 ACTGG ACTGG(1/-1)
BssSI 619 CACGAG CACGAG(-5/-1)
BstEII 699 GGTCACC G^GT[ACGT]ACC
BstPI 699 GGTCACC G^GT[ACGT]ACC
BstXI 848 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 510 GCTCAGC GC^T[ACGT]AGC
EcoO65I 699 GGTCACC G^GT[ACGT]ACC
EcoT14I 848 CCAAGG C^C[AT][AT]GG
Hin1I 780 GGCGTC G[GA]^CG[CT]C
HinfI 388 GACTC G^A[ACGT]TC
MaeI 13 CTAG C^TAG
MslI 648 CATCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PleI 388 GACTC GAGTC(4/5)
PmaCI 171 CACGTG CAC^GTG
PmlI 171 CACGTG CAC^GTG
PvuII 307 CAGCTG CAG^CTG
RcaI 287 TCATGA T^CATGA
RsaI 438 GTAC GT^AC
SbfI 567 CCTGCAGG CCTGCA^GG
Sse8387I 567 CCTGCAGG CCTGCA^GG
StyI 848 CCAAGG C^C[AT][AT]GG
Tsp509I 8 AATT ^AATT
TspRI 626 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 219 GGTCC G^G[AT]CC
885 GGTCC
BanII 402 GAGCCC G[GA]GC[CT]^C
445 GAGCCC
BbsI 295 GTCTTC GAAGAC(2/6)
928 GTCTTC
BglI 688 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
800 GCCTGGCGGGC
BmyI 402 GAGCCC G[AGT]GC[ACT]^C
445 GAGCCC
BpuAI 295 GTCTTC GAAGAC(2/6)
928 GTCTTC
BsaAI 171 CACGTG [CT]AC^GT[GA]
439 TACGTG
Bsp1286I 402 GAGCCC G[AGT]GC[ACT]^C
445 GAGCCC
BssHII 419 GCGCGC G^CGCGC
489 GCGCGC
BstF5I 267 CATCC GGATG(2/0)
923 GGATG
BstYI 125 AGATCC [GA]^GATC[CT]
839 AGATCC
EcoNI 567 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
843 CCTGGCCAAGG
FokI 267 CATCC GGATG(9/13)
923 GGATG
HaeII 709 AGCGCC [GA]GCGC^[CT]
754 AGCGCC
HgaI 682 GACGC GACGC(5/10)
781 GCGTC
MaeIII 700 GTCAC ^GT[ACGT]AC
869 GTGAC
MflI 125 AGATCC [GA]^GATC[CT]
839 AGATCC
PpuMI 218 AGGTCCT [GA]G^G[AT]CC[CT]
884 AGGTCCT
PstI 568 CTGCAG CTGCA^G
793 CTGCAG
SfaNI 647 GCATC GCATC(5/9)
728 GCATC
SfcI 568 CTGCAG C^T[GA][CT]AG
793 CTGCAG
SmlI 880 CTCAAG C^T[CT][GA]AG
934 CTCAAG
Tsp45I 700 GTCAC ^GT[GC]AC
869 GTGAC
XcmI 266 CCATCCTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
713 CCATGATCCCCCTGG
XhoII 125 AGATCC [GA]^GATC[CT]
839 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwI BalI
BbvI BcnI BpmI BsaJI BseRI
BsiYI BslI BsmAI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TaqI TseI TthHB8I