Restriction map for
pF1KB8946
##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI ApoI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI HphI
KpnI Ksp632I MaeI MamI MfeI
MluI MroI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SapI SexAI SfaNI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI StyI SwaI
TfiI XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 35 ACATGT A^C[GA][CT]GT
Alw44I 265 GTGCAC G^TGCAC
AlwI 756 GATCC GGATC(4/5)
AlwNI 270 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 731 AGCGCT AGC^GCT
ApaI 502 GGGCCC GGGCC^C
ApaLI 265 GTGCAC G^TGCAC
AscI 553 GGCGCGCC GG^CGCGCC
AspI 108 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaI 902 GAGACC GGTCTC(1/5)
BsiEI 296 CGGCCG CG[GA][CT]^CG
BspLU11I 35 ACATGT A^CATGT
BspMI 149 ACCTGC ACCTGC(4/8)
BsrBI 631 GAGCGG CCGCTC(-3/-3)
BssHII 554 GCGCGC G^CGCGC
BssSI 262 CTCGTG CACGAG(-5/-1)
BstYI 755 AGATCC [GA]^GATC[CT]
EagI 296 CGGCCG C^GGCCG
EclXI 296 CGGCCG C^GGCCG
Eco47III 731 AGCGCT AGC^GCT
Eco52I 296 CGGCCG C^GGCCG
HinfI 667 GAGTC G^A[ACGT]TC
MaeIII 178 GTGAC ^GT[ACGT]AC
MflI 755 AGATCC [GA]^GATC[CT]
PflFI 108 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 209 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 667 GAGTC GAGTC(4/5)
PstI 280 CTGCAG CTGCA^G
SbfI 279 CCTGCAGG CCTGCA^GG
ScaI 914 AGTACT AGT^ACT
SmaI 478 CCCGGG CCC^GGG
SphI 827 GCATGC GCATG^C
Sse8387I 279 CCTGCAGG CCTGCA^GG
Tsp45I 178 GTGAC ^GT[GC]AC
Tth111I 108 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 209 CCACTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 755 AGATCC [GA]^GATC[CT]
XmaCI 478 CCCGGG C^CCGGG
XmaI 478 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 340 GTCTAC GT^[AC][GT]AC
790 GTCTAC
BalI 320 TGGCCA TGG^CCA
711 TGGCCA
BbeI 285 GGCGCC GGCGC^C
641 GGCGCC
BbsI 114 GTCTTC GAAGAC(2/6)
672 GTCTTC
BpmI 81 CTCCAG CTGGAG(16/14)
705 CTCCAG
BpuAI 114 GTCTTC GAAGAC(2/6)
672 GTCTTC
BsmBI 63 CGTCTC CGTCTC(1/5)
604 GAGACG
BsrI 423 CCAGT ACTGG(1/-1)
707 CCAGT
BstNI 156 CCAGG CC^[AT]GG
307 CCTGG
DdeI 686 CTGAG C^T[ACGT]AG
921 CTCAG
DpnII 756 GATC ^GATC
844 GATC
DraII 501 GGGGCCC [GA]G^G[ACGT]CC[CT]
502 GGGCCCC
EcoNI 150 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
405 CCTGCCACAGG
EcoO109I 501 GGGGCCC [GA]G^G[ACGT]CC[CT]
502 GGGCCCC
EcoRII 156 CCAGG ^CC[AT]GG
307 CCTGG
HgaI 401 GCGTC GACGC(5/10)
788 GCGTC
KasI 285 GGCGCC G^GCGCC
641 GGCGCC
MboI 756 GATC ^GATC
844 GATC
MluNI 320 TGGCCA TGG^CCA
711 TGGCCA
MscI 320 TGGCCA TGG^CCA
711 TGGCCA
MseI 797 TTAA T^TAA
896 TTAA
MvaI 156 CCAGG CC^[AT]GG
307 CCTGG
NarI 285 GGCGCC GG^CGCC
641 GGCGCC
NdeII 756 GATC ^GATC
844 GATC
NlaIII 36 CATG CATG^
828 CATG
NspI 35 ACATGT [GA]CATG^[CT]
827 GCATGC
SacI 259 GAGCTC GAGCT^C
327 GAGCTC
Sau3AI 756 GATC ^GATC
844 GATC
SfcI 280 CTGCAG C^T[GA][CT]AG
378 CTATAG
SfoI 285 GGCGCC GGC^GCC
641 GGCGCC
TaqI 419 TCGA T^CGA
431 TCGA
Tru9I 797 TTAA T^TAA
896 TTAA
Tsp509I 373 AATT ^AATT
894 AATT
TthHB8I 419 TCGA T^CGA
431 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssKI
BstUI Cac8I CfoI Cfr10I Cfr13I
DsaI EaeI FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI KspI MaeII MboII
MnlI MspA1I MspI MvnI MwoI
NaeI NciI NgoMIV NlaIV RsaI
SacII Sau96I ScrFI TseI TspRI