Restriction map for
pF1KB8945
##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AclI AgeI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I AspI AvrII BalI
BamHI BanII BbrPI BbsI BciVI
BclI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsiWI BsmI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DraII DraIII DrdI
Eco47III Eco81I EcoNI EcoO109I EcoRV
EcoT22I FbaI FseI HindIII HpaI
MboI MfeI MflI MluI MluNI
MscI MslI MunI NdeI NdeII
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SapI Sau3AI SbfI
ScaI SexAI SfcI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
Acc65I 594 GGTACC G^GTACC
AccI 19 GTCGAC GT^[AC][GT]AC
AccIII 689 TCCGGA T^CCGGA
AcsI 658 GAATTC [GA]^AATT[CT]
AflII 313 CTTAAG C^TTAAG
AflIII 671 ACATGT A^C[GA][CT]GT
AhdI 862 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 647 AGCT AG^CT
Alw44I 835 GTGCAC G^TGCAC
ApaLI 835 GTGCAC G^TGCAC
ApoI 658 GAATTC [GA]^AATT[CT]
Asp718I 594 GGTACC G^GTACC
AspEI 862 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 835 GTGCAC G[AT]GC[AT]^C
AvaII 543 GGACC G^G[AT]CC
AviII 372 TGCGCA TGC^GCA
BbeI 229 GGCGCC GGCGC^C
BbvCI 878 CCTCAGC CCTCAGC(-5/-2)
BfaI 629 CTAG C^TAG
BfrI 313 CTTAAG C^TTAAG
BsaBI 696 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 689 TCCGGA [AT]^CCGG[AT]
BseAI 689 TCCGGA T^CCGGA
BseRI 210 CTCCTC GAGGAG(10/8)
BsiHKAI 835 GTGCAC G[AT]GC[AT]^C
BsmBI 152 CGTCTC CGTCTC(1/5)
BsmFI 341 GGGAC GGGAC(10/14)
BspEI 689 TCCGGA T^CCGGA
BspLU11I 671 ACATGT A^CATGT
BsrBI 162 GAGCGG CCGCTC(-3/-3)
BsrI 321 CCAGT ACTGG(1/-1)
BssHII 844 GCGCGC G^CGCGC
BstEII 258 GGTTACC G^GT[ACGT]ACC
BstPI 258 GGTTACC G^GT[ACGT]ACC
BstXI 423 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 879 CTCAG C^T[ACGT]AG
Eam1105I 862 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 258 GGTTACC G^GT[ACGT]ACC
EcoRI 658 GAATTC G^AATTC
FspI 372 TGCGCA TGC^GCA
HaeII 229 GGCGCC [GA]GCGC^[CT]
HincII 19 GTCGAC GT[CT]^[GA]AC
HindII 19 GTCGAC GT[CT]^[GA]AC
KasI 229 GGCGCC G^GCGCC
KpnI 594 GGTACC GGTAC^C
MaeI 629 CTAG C^TAG
MaeII 536 ACGT A^CGT
MamI 696 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 689 TCCGGA T^CCGGA
MseI 314 TTAA T^TAA
NarI 229 GGCGCC GG^CGCC
NcoI 569 CCATGG C^CATGG
NotI 472 GCGGCCGC GC^GGCCGC
SalI 19 GTCGAC G^TCGAC
SfaNI 375 GCATC GCATC(5/9)
SfiI 474 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 229 GGCGCC GGC^GCC
SmaI 79 CCCGGG CCC^GGG
SmlI 313 CTTAAG C^T[CT][GA]AG
SphI 107 GCATGC GCATG^C
TfiI 554 GATTC G^A[AT]TC
Tru9I 314 TTAA T^TAA
Tsp509I 659 AATT ^AATT
XmaCI 79 CCCGGG C^CCGGG
XmaI 79 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 229 GGCGCC G[GA]^CG[CT]C
583 GACGCC
AfaI 595 GTAC GT^AC
675 GTAC
AvaI 79 CCCGGG C^[CT]CG[GA]G
215 CTCGGG
BcgI 207 GCACTCCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
815 GCAAGTGCGTCG
BpmI 643 CTGGAG CTGGAG(16/14)
649 CTGGAG
BsaHI 229 GGCGCC G[GA]^CG[CT]C
583 GACGCC
BsgI 205 CTGCAC GTGCAG(16/14)
516 CTGCAC
BsiEI 437 CGGCCG CG[GA][CT]^CG
473 CGGCCG
BsmAI 153 GTCTC GTCTC(1/5)
681 GTCTC
BsoBI 79 CCCGGG C^[CT]CG[GA]G
215 CTCGGG
BspMI 203 ACCTGC ACCTGC(4/8)
732 GCAGGT
BsrFI 232 GCCGGC [GA]^CCGG[CT]
873 GCCGGC
Cfr10I 232 GCCGGC [GA]^CCGG[CT]
873 GCCGGC
EaeI 437 CGGCCG [CT]^GGCC[GA]
473 CGGCCG
EagI 437 CGGCCG C^GGCCG
473 CGGCCG
EarI 327 CTCTTC CTCTTC(1/4)
663 CTCTTC
EclXI 437 CGGCCG C^GGCCG
473 CGGCCG
Eco52I 437 CGGCCG C^GGCCG
473 CGGCCG
EcoT14I 485 CCTTGG C^C[AT][AT]GG
569 CCATGG
Hin1I 229 GGCGCC G[GA]^CG[CT]C
583 GACGCC
Ksp632I 327 CTCTTC CTCTTC(1/4)
663 CTCTTC
NaeI 232 GCCGGC GCC^GGC
873 GCCGGC
NgoMIV 232 GCCGGC G^CCGGC
873 GCCGGC
NspI 107 GCATGC [GA]CATG^[CT]
671 ACATGT
RsaI 595 GTAC GT^AC
675 GTAC
StyI 485 CCTTGG C^C[AT][AT]GG
569 CCATGG
TaqI 20 TCGA T^CGA
684 TCGA
Tsp45I 193 GTCAC ^GT[GC]AC
803 GTCAC
TthHB8I 20 TCGA T^CGA
684 TCGA
XcmI 527 CCACCTACAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
591 CCAGGTACCCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI BanI BbvI BcnI
BglI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DsaI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KspI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
SacII Sau96I ScrFI TseI TspRI