##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
ApoI        AseI        AsnI        AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BfaI        
BfrI        BglI        BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        MaeI        MamI        MfeI        
MluI        MroI        MseI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        464  GGTACC               G^GTACC
AscI          784  GGCGCGCC             GG^CGCGCC
Asp700I       115  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       464  GGTACC               G^GTACC
BcgI          706  GCAGCAGTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         530  AGATCT               A^GATCT
BlpI            6  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        6  GCTGAGC              GC^T[ACGT]AGC
BsgI          704  GTGCAG               GTGCAG(16/14)
BsmAI         564  GAGAC                GTCTC(1/5)
BsmI          849  GAATGC               GAATGC(1/-1)
BstAPI        650  GCAAGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         530  AGATCT               [GA]^GATC[CT]
CelII           6  GCTGAGC              GC^T[ACGT]AGC
DpnII         531  GATC                 ^GATC
EarI          105  GAAGAG               CTCTTC(1/4)
EcoT14I       380  CCAAGG               C^C[AT][AT]GG
KpnI          464  GGTACC               GGTAC^C
Ksp632I       105  GAAGAG               CTCTTC(1/4)
MaeII         514  ACGT                 A^CGT
MboI          531  GATC                 ^GATC
MboII         105  GAAGA                GAAGA(8/7)
MflI          530  AGATCT               [GA]^GATC[CT]
NdeII         531  GATC                 ^GATC
SapI          105  GAAGAGC              GCTCTTC(1/4)
Sau3AI        531  GATC                 ^GATC
SmaI          667  CCCGGG               CCC^GGG
StyI          380  CCAAGG               C^C[AT][AT]GG
XhoII         530  AGATCT               [GA]^GATC[CT]
XmaCI         667  CCCGGG               C^CCGGG
XmaI          667  CCCGGG               C^CCGGG
XmnI          115  GAAAACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI          126  GTAC                 GT^AC
              465  GTAC                
ApaI          663  GGGCCC               GGGCC^C
              670  GGGCCC              
BpmI           55  CTGGAG               CTGGAG(16/14)
              439  CTGGAG              
BsmFI         310  GGGAC                GGGAC(10/14)
              224  GTCCC               
BssHII        500  GCGCGC               G^CGCGC
              785  GCGCGC              
DdeI            7  CTGAG                C^T[ACGT]AG
              130  CTGAG               
DsaI           77  CCGCGG               C^C[GA][CT]GG
               94  CCGCGG              
HphI          187  GGTGA                GGTGA(8/7)
               17  TCACC               
KspI           77  CCGCGG               CCGC^GG
               94  CCGCGG              
MaeIII         16  GTCAC                ^GT[ACGT]AC
              188  GTGAC               
PpuMI         222  GGGTCCC              [GA]G^G[AT]CC[CT]
              795  GGGTCCT             
PstI          505  CTGCAG               CTGCA^G
              883  CTGCAG              
RsaI          126  GTAC                 GT^AC
              465  GTAC                
SacII          77  CCGCGG               CCGC^GG
               94  CCGCGG              
Tsp45I         16  GTCAC                ^GT[GC]AC
              188  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsoBI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HpaII       ItaI        MluNI       
MnlI        MscI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIV       Sau96I      
ScrFI       SfcI        TseI        TspRI