Restriction map for
pF1KB8938
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DpnII
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MslI MunI
NarI NcoI NdeI NdeII NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI Sau3AI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tth111I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 587 GTAGAC GT^[AC][GT]AC
AlwNI 578 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 534 GAGCTC G[AT]GC[AT]^C
AvaI 112 CCCGAG C^[CT]CG[GA]G
AvaII 513 GGACC G^G[AT]CC
BciVI 412 GGATAC GTATCC(6/5)
BglI 310 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 838 CTGGAG CTGGAG(16/14)
BsaI 567 GAGACC GGTCTC(1/5)
BsiHKAI 534 GAGCTC G[AT]GC[AT]^C
BsmBI 539 CGTCTC CGTCTC(1/5)
BsoBI 112 CCCGAG C^[CT]CG[GA]G
BsrFI 175 GCCGGC [GA]^CCGG[CT]
Cfr10I 175 GCCGGC [GA]^CCGG[CT]
DraII 512 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 559 CCGTGG C^C[GA][CT]GG
EarI 593 GAAGAG CTCTTC(1/4)
EcoO109I 512 AGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 169 AGCGCC [GA]GCGC^[CT]
HinfI 680 GAATC G^A[ACGT]TC
Ksp632I 593 GAAGAG CTCTTC(1/4)
NaeI 175 GCCGGC GCC^GGC
NgoMIV 175 GCCGGC G^CCGGC
PflMI 831 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 512 AGGACCC [GA]G^G[AT]CC[CT]
PstI 151 CTGCAG CTGCA^G
SacI 534 GAGCTC GAGCT^C
ScaI 275 AGTACT AGT^ACT
SfaNI 339 GCATC GCATC(5/9)
SspI 502 AATATT AAT^ATT
StuI 858 AGGCCT AGG^CCT
TaqI 683 TCGA T^CGA
TfiI 680 GAATC G^A[AT]TC
TspRI 495 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 683 TCGA T^CGA
Van91I 831 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 627 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 643 GAATTT [GA]^AATT[CT]
778 AAATTT
ApoI 643 GAATTT [GA]^AATT[CT]
778 AAATTT
BanII 394 GAGCCC G[GA]GC[CT]^C
534 GAGCTC
BbsI 599 GAAGAC GAAGAC(2/6)
844 GAAGAC
BfaI 628 CTAG C^TAG
700 CTAG
BmyI 394 GAGCCC G[AGT]GC[ACT]^C
534 GAGCTC
BpuAI 599 GAAGAC GAAGAC(2/6)
844 GAAGAC
BseRI 329 CTCCTC GAGGAG(10/8)
332 CTCCTC
BsiEI 123 CGGCCG CG[GA][CT]^CG
302 CGGCCG
BsmFI 238 GGGAC GGGAC(10/14)
222 GTCCC
Bsp1286I 394 GAGCCC G[AGT]GC[ACT]^C
534 GAGCTC
BsrI 241 ACTGG ACTGG(1/-1)
494 CCAGT
BstF5I 340 CATCC GGATG(2/0)
563 GGATG
Cfr13I 267 GGGCC G^G[ACGT]CC
513 GGACC
EaeI 123 CGGCCG [CT]^GGCC[GA]
302 CGGCCG
EagI 123 CGGCCG C^GGCCG
302 CGGCCG
EclXI 123 CGGCCG C^GGCCG
302 CGGCCG
Eco52I 123 CGGCCG C^GGCCG
302 CGGCCG
FokI 340 CATCC GGATG(9/13)
563 GGATG
HphI 801 GGTGA GGTGA(8/7)
707 TCACC
MaeI 628 CTAG C^TAG
700 CTAG
MseI 526 TTAA T^TAA
653 TTAA
MspA1I 22 CCGCTG C[AC]G^C[GT]G
305 CCGCTG
Sau96I 267 GGGCC G^G[ACGT]CC
513 GGACC
SfcI 151 CTGCAG C^T[GA][CT]AG
609 CTACAG
Tru9I 526 TTAA T^TAA
653 TTAA
Tsp509I 644 AATT ^AATT
779 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AfaI AluI BbvI
BcnI BsaJI BsiYI BslI BsmAI
BssKI BstNI BstUI Cac8I CfoI
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIV RsaI ScrFI
TseI Tsp45I