Restriction map for
pF1KB8934
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HindIII HpaI KpnI MaeI
MfeI MluI MroI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SalI SapI SbfI ScaI SfcI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 216 GACGTC GACGT^C
AflIII 434 ACGTGT A^C[GA][CT]GT
AlwI 153 GGATC GGATC(4/5)
AlwNI 448 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 841 AGCGCT AGC^GCT
AvaII 574 GGTCC G^G[AT]CC
BalI 419 TGGCCA TGG^CCA
BbeI 114 GGCGCC GGCGC^C
BglI 310 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 743 AGATCT A^GATCT
BsaBI 777 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 445 GTGCAG GTGCAG(16/14)
BsmAI 334 GAGAC GTCTC(1/5)
BsmI 659 GCATTC GAATGC(1/-1)
BsrDI 183 GCAATG GCAATG(2/0)
BstAPI 589 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 27 GGATG GGATG(2/0)
BstXI 422 CCAGTTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 574 GGTCC G^G[ACGT]CC
DdeI 374 CTGAG C^T[ACGT]AG
DraII 573 AGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 352 GAAGAG CTCTTC(1/4)
Eco47III 841 AGCGCT AGC^GCT
EcoO109I 573 AGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 27 GGATG GGATG(9/13)
HgaI 625 GACGC GACGC(5/10)
HincII 136 GTCAAC GT[CT]^[GA]AC
HindII 136 GTCAAC GT[CT]^[GA]AC
KasI 114 GGCGCC G^GCGCC
Ksp632I 352 GAAGAG CTCTTC(1/4)
KspI 329 CCGCGG CCGC^GG
MamI 777 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 419 TGGCCA TGG^CCA
MscI 419 TGGCCA TGG^CCA
NarI 114 GGCGCC GG^CGCC
NotI 644 GCGGCCGC GC^GGCCGC
PpuMI 573 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 282 GAGCTC GAGCT^C
SacII 329 CCGCGG CCGC^GG
Sau96I 574 GGTCC G^G[ACGT]CC
SexAI 570 ACCAGGT A^CC[AT]GGT
SfaNI 126 GATGC GCATC(5/9)
SfoI 114 GGCGCC GGC^GCC
TaqI 44 TCGA T^CGA
TfiI 75 GAATC G^A[AT]TC
Tsp509I 13 AATT ^AATT
TspRI 372 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 44 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 282 GAGCTC G[AT]GC[AT]^C
458 GTGCTC
BanI 114 GGCGCC G^G[CT][GA]CC
204 GGTGCC
BsiEI 344 CGGCCG CG[GA][CT]^CG
645 CGGCCG
BsiHKAI 282 GAGCTC G[AT]GC[AT]^C
458 GTGCTC
BsrBI 59 GAGCGG CCGCTC(-3/-3)
694 GAGCGG
BsrI 422 CCAGT ACTGG(1/-1)
507 CCAGT
BstYI 153 GGATCT [GA]^GATC[CT]
743 AGATCT
DpnII 154 GATC ^GATC
744 GATC
DsaI 329 CCGCGG C^C[GA][CT]GG
341 CCACGG
EagI 344 CGGCCG C^GGCCG
645 CGGCCG
EclXI 344 CGGCCG C^GGCCG
645 CGGCCG
Eco52I 344 CGGCCG C^GGCCG
645 CGGCCG
EcoRV 40 GATATC GAT^ATC
395 GATATC
HphI 831 GGTGA GGTGA(8/7)
722 TCACC
MaeII 217 ACGT A^CGT
434 ACGT
MboI 154 GATC ^GATC
744 GATC
MflI 153 GGATCT [GA]^GATC[CT]
743 AGATCT
NdeII 154 GATC ^GATC
744 GATC
NlaIII 194 CATG CATG^
478 CATG
PflMI 193 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
411 CCAGCCTATGG
Sau3AI 154 GATC ^GATC
744 GATC
Van91I 193 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
411 CCAGCCTATGG
XcmI 189 CCACCCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
571 CCAGGTCCCTTTTGG
XhoII 153 GGATCT [GA]^GATC[CT]
743 AGATCT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI EaeI EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeIII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
PleI RsaI ScrFI StyI TseI