Restriction map for
pF1KB8931
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI ApaLI ApoI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DsaI
Eam1105I EarI Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HindIII HpaI HphI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp509I
Tth111I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 620 GGTACC G^GTACC
AcyI 541 GGCGCC G[GA]^CG[CT]C
AflIII 146 ACGTGT A^C[GA][CT]GT
AlwNI 490 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 366 AGCGCT AGC^GCT
ApaI 355 GGGCCC GGGCC^C
AscI 610 GGCGCGCC GG^CGCGCC
Asp718I 620 GGTACC G^GTACC
AspHI 150 GTGCTC G[AT]GC[AT]^C
AvaII 463 GGACC G^G[AT]CC
BbeI 541 GGCGCC GGCGC^C
BbrPI 243 CACGTG CAC^GTG
BglI 384 GCCGTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 776 AGATCT A^GATCT
BsaAI 243 CACGTG [CT]AC^GT[GA]
BsaHI 541 GGCGCC G[GA]^CG[CT]C
BsaWI 791 ACCGGA [AT]^CCGG[AT]
BseRI 328 GAGGAG GAGGAG(10/8)
BsiEI 237 CGGCCG CG[GA][CT]^CG
BsiHKAI 150 GTGCTC G[AT]GC[AT]^C
BsmFI 516 GTCCC GGGAC(10/14)
BspMI 768 GCAGGT ACCTGC(4/8)
BsrBI 636 CCGCTC CCGCTC(-3/-3)
BsrDI 182 GCAATG GCAATG(2/0)
BsrFI 544 GCCGGC [GA]^CCGG[CT]
BstBI 663 TTCGAA TT^CGAA
BstF5I 51 CATCC GGATG(2/0)
BstYI 776 AGATCT [GA]^GATC[CT]
Cfr10I 544 GCCGGC [GA]^CCGG[CT]
DrdI 763 GACCAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 237 CGGCCG C^GGCCG
EclXI 237 CGGCCG C^GGCCG
Eco47III 366 AGCGCT AGC^GCT
Eco52I 237 CGGCCG C^GGCCG
FokI 51 CATCC GGATG(9/13)
HgaI 91 GCGTC GACGC(5/10)
Hin1I 541 GGCGCC G[GA]^CG[CT]C
HincII 703 GTCAAC GT[CT]^[GA]AC
HindII 703 GTCAAC GT[CT]^[GA]AC
KasI 541 GGCGCC G^GCGCC
KpnI 620 GGTACC GGTAC^C
MboII 645 GAAGA GAAGA(8/7)
MflI 776 AGATCT [GA]^GATC[CT]
NaeI 544 GCCGGC GCC^GGC
NarI 541 GGCGCC GG^CGCC
NgoMIV 544 GCCGGC G^CCGGC
NspV 663 TTCGAA TT^CGAA
PflMI 489 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 505 GACTC GAGTC(4/5)
PmaCI 243 CACGTG CAC^GTG
PmlI 243 CACGTG CAC^GTG
PpuMI 462 AGGACCT [GA]G^G[AT]CC[CT]
SfoI 541 GGCGCC GGC^GCC
SfuI 663 TTCGAA TT^CGAA
SgrAI 543 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 664 TCGA T^CGA
Tsp45I 761 GTGAC ^GT[GC]AC
TspRI 215 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 664 TCGA T^CGA
Van91I 489 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 776 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 414 GTAC GT^AC
621 GTAC
BanI 541 GGCGCC G^G[CT][GA]CC
620 GGTACC
BanII 355 GGGCCC G[GA]GC[CT]^C
595 GGGCTC
BsmAI 62 GAGAC GTCTC(1/5)
219 GTCTC
BssHII 256 GCGCGC G^CGCGC
611 GCGCGC
BstNI 161 CCTGG CC^[AT]GG
617 CCTGG
DdeI 741 CTCAG C^T[ACGT]AG
757 CTTAG
DpnII 627 GATC ^GATC
777 GATC
DraII 314 GGGGCCT [GA]G^G[ACGT]CC[CT]
462 AGGACCT
EcoO109I 314 GGGGCCT [GA]G^G[ACGT]CC[CT]
462 AGGACCT
EcoRII 161 CCTGG ^CC[AT]GG
617 CCTGG
MaeII 146 ACGT A^CGT
244 ACGT
MaeIII 278 GTTAC ^GT[ACGT]AC
761 GTGAC
MboI 627 GATC ^GATC
777 GATC
MvaI 161 CCTGG CC^[AT]GG
617 CCTGG
NdeII 627 GATC ^GATC
777 GATC
RsaI 414 GTAC GT^AC
621 GTAC
Sau3AI 627 GATC ^GATC
777 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI AvaI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstUI Cac8I CfoI Cfr13I EaeI
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MnlI MspA1I MspI MvnI MwoI
NciI NlaIV Sau96I ScrFI SmaI
TfiI TseI XmaCI XmaI