Restriction map for
pF1KB8926
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AvaI AvaII
AviII AvrII BalI BanI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstEII BstF5I
BstPI BstUI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FokI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 740 GCGG CCGC(-3/-1)
AclI 276 AACGTT AA^CGTT
AcsI 141 AAATTC [GA]^AATT[CT]
AfaI 656 GTAC GT^AC
AhdI 218 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 141 AAATTC [GA]^AATT[CT]
AspEI 218 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 118 GGATCC G^GATCC
BbsI 186 GAAGAC GAAGAC(2/6)
BbvI 797 GCTGC GCAGC(8/12)
BciVI 374 GGATAC GTATCC(6/5)
BclI 573 TGATCA T^GATCA
BpmI 209 CTGGAG CTGGAG(16/14)
BpuAI 186 GAAGAC GAAGAC(2/6)
BsaJI 683 CCAGGG C^C[ACGT][ACGT]GG
BsgI 663 GTGCAG GTGCAG(16/14)
BssSI 451 CACGAG CACGAG(-5/-1)
BstBI 183 TTCGAA TT^CGAA
BstXI 237 CCAGGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 118 GGATCC [GA]^GATC[CT]
Cfr13I 344 GGGCC G^G[ACGT]CC
DdeI 107 CTGAG C^T[ACGT]AG
EaeI 741 CGGCCA [CT]^GGCC[GA]
Eam1105I 218 GACAAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 133 GATATC GAT^ATC
FbaI 573 TGATCA T^GATCA
HapII 258 CCGG C^CGG
HincII 428 GTCAAC GT[CT]^[GA]AC
HindII 428 GTCAAC GT[CT]^[GA]AC
HpaII 258 CCGG C^CGG
MaeII 277 ACGT A^CGT
MflI 118 GGATCC [GA]^GATC[CT]
MseI 610 TTAA T^TAA
MspI 258 CCGG C^CGG
NlaIV 118 GGATCC GG[ACGT]^[ACGT]CC
NspV 183 TTCGAA TT^CGAA
Psp1406I 276 AACGTT AA^CGTT
RsaI 656 GTAC GT^AC
Sau96I 344 GGGCC G^G[ACGT]CC
SfuI 183 TTCGAA TT^CGAA
SmlI 123 CTCAAG C^T[CT][GA]AG
StuI 636 AGGCCT AGG^CCT
TaqI 184 TCGA T^CGA
Tru9I 610 TTAA T^TAA
TseI 797 GCTGC G^C[AT]GC
TthHB8I 184 TCGA T^CGA
XhoII 118 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 318 GTGCAC G^TGCAC
784 GTGCAC
AlwI 119 GATCC GGATC(4/5)
118 GGATC
ApaLI 318 GTGCAC G^TGCAC
784 GTGCAC
Asp700I 417 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
669 GAAGAGTTTC
AspHI 318 GTGCAC G[AT]GC[AT]^C
784 GTGCAC
BmyI 318 GTGCAC G[AGT]GC[ACT]^C
784 GTGCAC
BsiHKAI 318 GTGCAC G[AT]GC[AT]^C
784 GTGCAC
Bsp1286I 318 GTGCAC G[AGT]GC[ACT]^C
784 GTGCAC
DpnII 119 GATC ^GATC
574 GATC
EarI 152 GAAGAG CTCTTC(1/4)
669 GAAGAG
Fnu4HI 740 GCGGC GC^[ACGT]GC
797 GCTGC
ItaI 740 GCGGC GC^[ACGT]GC
797 GCTGC
Ksp632I 152 GAAGAG CTCTTC(1/4)
669 GAAGAG
MboI 119 GATC ^GATC
574 GATC
MslI 91 CACAAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
547 CACAGTGGTG
NdeII 119 GATC ^GATC
574 GATC
Sau3AI 119 GATC ^GATC
574 GATC
XmnI 417 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
669 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsiYI BslI BsmAI BsrI
BssKI BstNI EcoRII HaeIII HindIII
HinfI HphI MaeIII MboII MnlI
MvaI NlaIII PleI ScrFI SfcI
TfiI Tsp509I TspRI