Restriction map for
pF1KB8914
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BamHI BbsI BbvCI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BpuAI BsaI BseAI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DrdI Eam1105I EarI
Eco47III Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I KspI MfeI MluI
MroI MslI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 397 GTCTAC GT^[AC][GT]AC
AgeI 448 ACCGGT A^CCGGT
Alw44I 418 GTGCAC G^TGCAC
ApaLI 418 GTGCAC G^TGCAC
AspHI 418 GTGCAC G[AT]GC[AT]^C
AvrII 84 CCTAGG C^CTAGG
BalI 731 TGGCCA TGG^CCA
BanII 148 GGGCTC G[GA]GC[CT]^C
BbrPI 421 CACGTG CAC^GTG
BglI 141 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 596 AGATCT A^GATCT
BlnI 84 CCTAGG C^CTAGG
BpmI 272 CTCCAG CTGGAG(16/14)
BsaAI 421 CACGTG [CT]AC^GT[GA]
BsiEI 389 CGGCCG CG[GA][CT]^CG
BsiHKAI 418 GTGCAC G[AT]GC[AT]^C
BspHI 5 TCATGA T^CATGA
BspMI 659 GCAGGT ACCTGC(4/8)
BsrBI 269 CCGCTC CCGCTC(-3/-3)
BsrI 504 ACTGG ACTGG(1/-1)
BssHII 119 GCGCGC G^CGCGC
BstXI 29 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 596 AGATCT [GA]^GATC[CT]
DdeI 699 CTCAG C^T[ACGT]AG
DraIII 567 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 389 CGGCCG C^GGCCG
EclXI 389 CGGCCG C^GGCCG
Eco52I 389 CGGCCG C^GGCCG
EcoNI 181 CCTTTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 426 GAATTC G^AATTC
EcoT14I 84 CCTAGG C^C[AT][AT]GG
FauI 225 GCGGG CCCGC(4/6)
MaeII 422 ACGT A^CGT
MflI 596 AGATCT [GA]^GATC[CT]
MluNI 731 TGGCCA TGG^CCA
MscI 731 TGGCCA TGG^CCA
MseI 524 TTAA T^TAA
MspA1I 192 CAGCGG C[AC]G^C[GT]G
NlaIII 6 CATG CATG^
PinAI 448 ACCGGT A^CCGGT
PmaCI 421 CACGTG CAC^GTG
PmlI 421 CACGTG CAC^GTG
RcaI 5 TCATGA T^CATGA
SfcI 336 CTACAG C^T[GA][CT]AG
SmlI 379 CTCAAG C^T[CT][GA]AG
StyI 84 CCTAGG C^C[AT][AT]GG
Tru9I 524 TTAA T^TAA
XhoII 596 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 120 CGCG CG^CG
224 CGCG
AcsI 426 GAATTC [GA]^AATT[CT]
514 GAATTT
AluI 291 AGCT AG^CT
641 AGCT
ApoI 426 GAATTC [GA]^AATT[CT]
514 GAATTT
BfaI 85 CTAG C^TAG
499 CTAG
BmyI 148 GGGCTC G[AGT]GC[ACT]^C
418 GTGCAC
BsaBI 552 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
591 GATCAAGATC
BsaWI 448 ACCGGT [AT]^CCGG[AT]
611 ACCGGA
BsmFI 370 GGGAC GGGAC(10/14)
468 GTCCC
Bsp1286I 148 GGGCTC G[AGT]GC[ACT]^C
418 GTGCAC
BstF5I 407 GGATG GGATG(2/0)
617 GGATG
BstUI 120 CGCG CG^CG
224 CGCG
DsaI 157 CCACGG C^C[GA][CT]GG
392 CCGTGG
EaeI 389 CGGCCG [CT]^GGCC[GA]
731 TGGCCA
FokI 407 GGATG GGATG(9/13)
617 GGATG
MaeI 85 CTAG C^TAG
499 CTAG
MaeIII 230 GTCAC ^GT[ACGT]AC
260 GTCAC
MamI 552 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
591 GATCAAGATC
MboII 411 GAAGA GAAGA(8/7)
676 TCTTC
MvnI 120 CGCG CG^CG
224 CGCG
NaeI 309 GCCGGC GCC^GGC
387 GCCGGC
NgoMIV 309 GCCGGC G^CCGGC
387 GCCGGC
SgrAI 308 CGCCGGCG C[GA]^CCGG[CT]G
447 CACCGGTG
Tsp45I 230 GTCAC ^GT[GC]AC
260 GTCAC
Tsp509I 427 AATT ^AATT
515 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcyI AvaI AvaII BanI
BbeI BbvI BcnI BsaHI BsaJI
BseRI BsiYI BslI BsoBI BsrFI
BssKI BstNI Cac8I CfoI Cfr10I
Cfr13I DpnII EcoRII Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MboI
MnlI MspI MvaI MwoI NarI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfoI SmaI TseI XmaCI
XmaI