Restriction map for
pF1KB8911
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraII DrdI DsaI Eam1105I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HindIII HpaI KspI MaeII
MamI MfeI MflI MroI MseI
MunI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SbfI ScaI SexAI
SfaNI SfcI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I TspRI Tth111I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 278 GGTACC G^GTACC
AcsI 476 AAATTT [GA]^AATT[CT]
AflIII 206 ACGCGT A^C[GA][CT]GT
AlwI 510 GGATC GGATC(4/5)
AlwNI 586 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 563 AGCGCT AGC^GCT
ApoI 476 AAATTT [GA]^AATT[CT]
AscI 367 GGCGCGCC GG^CGCGCC
Asp718I 278 GGTACC G^GTACC
AvaII 636 GGACC G^G[AT]CC
BfaI 598 CTAG C^TAG
BglI 181 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 661 CTCCTC GAGGAG(10/8)
BsiEI 266 CGGCCG CG[GA][CT]^CG
BsmAI 678 GAGAC GTCTC(1/5)
BspMI 275 GCAGGT ACCTGC(4/8)
BssHII 368 GCGCGC G^CGCGC
DraIII 646 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 266 CGGCCG C^GGCCG
EarI 549 CTCTTC CTCTTC(1/4)
EclXI 266 CGGCCG C^GGCCG
Eco47III 563 AGCGCT AGC^GCT
Eco52I 266 CGGCCG C^GGCCG
HincII 34 GTCGAC GT[CT]^[GA]AC
HindII 34 GTCGAC GT[CT]^[GA]AC
KpnI 278 GGTACC GGTAC^C
Ksp632I 549 CTCTTC CTCTTC(1/4)
MaeI 598 CTAG C^TAG
MaeIII 50 GTCAC ^GT[ACGT]AC
MluI 206 ACGCGT A^CGCGT
MslI 52 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI 585 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 102 GAGCTC GAGCT^C
SalI 34 GTCGAC G^TCGAC
SapI 548 GCTCTTC GCTCTTC(1/4)
SgrAI 167 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 324 CCCGGG CCC^GGG
Tsp45I 50 GTCAC ^GT[GC]AC
Tsp509I 477 AATT ^AATT
Van91I 585 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 324 CCCGGG C^CCGGG
XmaI 324 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 34 GTCGAC GT^[AC][GT]AC
229 GTCTAC
AspHI 102 GAGCTC G[AT]GC[AT]^C
127 GAGCAC
AvaI 324 CCCGGG C^[CT]CG[GA]G
400 CCCGAG
BalI 90 TGGCCA TGG^CCA
653 TGGCCA
BanII 102 GAGCTC G[GA]GC[CT]^C
198 GAGCCC
BlpI 18 GCTTAGC GC^T[ACGT]AGC
195 GCTGAGC
Bpu1102I 18 GCTTAGC GC^T[ACGT]AGC
195 GCTGAGC
BsgI 538 CTGCAC GTGCAG(16/14)
147 GTGCAG
BsiHKAI 102 GAGCTC G[AT]GC[AT]^C
127 GAGCAC
BsoBI 324 CCCGGG C^[CT]CG[GA]G
400 CCCGAG
BsrI 533 ACTGG ACTGG(1/-1)
93 CCAGT
BssSI 52 CACGAG CACGAG(-5/-1)
144 CTCGTG
BstF5I 243 CATCC GGATG(2/0)
710 GGATG
CelII 18 GCTTAGC GC^T[ACGT]AGC
195 GCTGAGC
DpnII 42 GATC ^GATC
511 GATC
FokI 243 CATCC GGATG(9/13)
710 GGATG
HinfI 37 GACTC G^A[ACGT]TC
659 GACTC
HphI 345 GGTGA GGTGA(8/7)
629 TCACC
MboI 42 GATC ^GATC
511 GATC
MboII 720 GAAGA GAAGA(8/7)
550 TCTTC
MluNI 90 TGGCCA TGG^CCA
653 TGGCCA
MscI 90 TGGCCA TGG^CCA
653 TGGCCA
MspA1I 193 CCGCTG C[AC]G^C[GT]G
358 CCGCTG
NaeI 264 GCCGGC GCC^GGC
300 GCCGGC
NdeII 42 GATC ^GATC
511 GATC
NgoMIV 264 GCCGGC G^CCGGC
300 GCCGGC
PleI 37 GACTC GAGTC(4/5)
659 GACTC
Sau3AI 42 GATC ^GATC
511 GATC
TaqI 35 TCGA T^CGA
40 TCGA
TthHB8I 35 TCGA T^CGA
40 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BbeI BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI KasI
MnlI MspI MvaI MvnI MwoI
NarI NciI NlaIV RsaI Sau96I
ScrFI SfoI TseI