Restriction map for
pF1KB8897
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BseAI BsgI BsiWI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FbaI
FseI HincII HindII HindIII HpaI
HphI KpnI MaeI MamI MfeI
MflI MluI MroI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SalI SbfI ScaI
SexAI SfcI SfuI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 379 GTCTAC GT^[AC][GT]AC
AcsI 402 GAATTC [GA]^AATT[CT]
AflIII 460 ACACGT A^C[GA][CT]GT
AlwNI 469 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 427 GGGCCC GGGCC^C
ApoI 402 GAATTC [GA]^AATT[CT]
AspHI 366 GTGCTC G[AT]GC[AT]^C
AvaI 301 CCCGAG C^[CT]CG[GA]G
AvaII 99 GGACC G^G[AT]CC
AviII 262 TGCGCA TGC^GCA
BalI 41 TGGCCA TGG^CCA
BanII 427 GGGCCC G[GA]GC[CT]^C
BcgI 527 GCATCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 139 CCGGG CC^[GC]GG
BlpI 621 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 621 GCTCAGC GC^T[ACGT]AGC
BsaWI 375 TCCGGT [AT]^CCGG[AT]
BsiHKAI 366 GTGCTC G[AT]GC[AT]^C
BsmBI 629 CGTCTC CGTCTC(1/5)
BsoBI 301 CCCGAG C^[CT]CG[GA]G
BssHII 323 GCGCGC G^CGCGC
CelII 621 GCTCAGC GC^T[ACGT]AGC
EarI 355 GAAGAG CTCTTC(1/4)
EcoRI 402 GAATTC G^AATTC
FspI 262 TGCGCA TGC^GCA
Ksp632I 355 GAAGAG CTCTTC(1/4)
KspI 307 CCGCGG CCGC^GG
MaeII 462 ACGT A^CGT
MluNI 41 TGGCCA TGG^CCA
MscI 41 TGGCCA TGG^CCA
NciI 139 CCGGG CC^[GC]GG
NlaIII 594 CATG CATG^
PflMI 468 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 307 CCGCGG CCGC^GG
SapI 355 GAAGAGC GCTCTTC(1/4)
SfiI 436 GGCCGGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 179 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 530 TCGA T^CGA
Tsp45I 34 GTCAC ^GT[GC]AC
Tsp509I 403 AATT ^AATT
TspRI 639 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 530 TCGA T^CGA
Van91I 468 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BbvI 285 GCAGC GCAGC(8/12)
393 GCAGC
BglI 123 GCCCTACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
437 GCCGGCGAGGC
BmyI 366 GTGCTC G[AGT]GC[ACT]^C
427 GGGCCC
BpmI 475 CTGGAG CTGGAG(16/14)
481 CTGGAG
BseRI 646 GAGGAG GAGGAG(10/8)
649 GAGGAG
BsiEI 198 CGGCCG CG[GA][CT]^CG
435 CGGCCG
Bsp1286I 366 GTGCTC G[AGT]GC[ACT]^C
427 GGGCCC
BsrBI 76 CCGCTC CCGCTC(-3/-3)
203 GAGCGG
BstF5I 389 GGATG GGATG(2/0)
398 GGATG
DpnII 534 GATC ^GATC
567 GATC
DsaI 307 CCGCGG C^C[GA][CT]GG
385 CCGTGG
EagI 198 CGGCCG C^GGCCG
435 CGGCCG
EclXI 198 CGGCCG C^GGCCG
435 CGGCCG
Eco52I 198 CGGCCG C^GGCCG
435 CGGCCG
FokI 389 GGATG GGATG(9/13)
398 GGATG
HinfI 55 GAGTC G^A[ACGT]TC
253 GAGTC
MaeIII 34 GTCAC ^GT[ACGT]AC
464 GTTAC
MboI 534 GATC ^GATC
567 GATC
MboII 355 GAAGA GAAGA(8/7)
655 GAAGA
NdeII 534 GATC ^GATC
567 GATC
PleI 55 GAGTC GAGTC(4/5)
253 GAGTC
Sau3AI 534 GATC ^GATC
567 GATC
SfaNI 390 GATGC GCATC(5/9)
527 GCATC
TseI 285 GCAGC G^C[AT]GC
393 GCAGC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbeI BsaHI BsaJI BsiYI BslI
BsmAI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI KasI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NgoMIV NlaIV Sau96I
ScrFI SfoI