Restriction map for
pF1KB8894
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MaeI MaeIII MamI MfeI
MflI MluI MroI MseI MslI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 385 GTAC GT^AC
Alw44I 37 GTGCAC G^TGCAC
AlwNI 96 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 37 GTGCAC G^TGCAC
BbsI 516 GAAGAC GAAGAC(2/6)
BpmI 637 CTGGAG CTGGAG(16/14)
BpuAI 516 GAAGAC GAAGAC(2/6)
BspMI 458 ACCTGC ACCTGC(4/8)
BsrBI 322 GAGCGG CCGCTC(-3/-3)
BsrFI 217 GCCGGC [GA]^CCGG[CT]
BssSI 43 CACGAG CACGAG(-5/-1)
BstF5I 375 CATCC GGATG(2/0)
Cfr10I 217 GCCGGC [GA]^CCGG[CT]
DdeI 335 CTCAG C^T[ACGT]AG
DpnII 510 GATC ^GATC
DraIII 28 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 129 CCATGG C^C[GA][CT]GG
EarI 537 GAAGAG CTCTTC(1/4)
FauI 388 CCCGC CCCGC(4/6)
FokI 375 CATCC GGATG(9/13)
HphI 291 GGTGA GGTGA(8/7)
Ksp632I 537 GAAGAG CTCTTC(1/4)
MboI 510 GATC ^GATC
NaeI 217 GCCGGC GCC^GGC
NcoI 129 CCATGG C^CATGG
NdeII 510 GATC ^GATC
NgoMIV 217 GCCGGC G^CCGGC
PflMI 27 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 102 CTGCAG CTGCA^G
PvuII 77 CAGCTG CAG^CTG
RsaI 385 GTAC GT^AC
SacI 640 GAGCTC GAGCT^C
Sau3AI 510 GATC ^GATC
SmaI 267 CCCGGG CCC^GGG
SmlI 643 CTCAAG C^T[CT][GA]AG
StuI 494 AGGCCT AGG^CCT
TspRI 575 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 27 CCACCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 267 CCCGGG C^CCGGG
XmaI 267 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 284 CGCG CG^CG
367 CGCG
AcyI 211 GGCGCC G[GA]^CG[CT]C
256 GGCGCC
ApaI 262 GGGCCC GGGCC^C
632 GGGCCC
AspHI 37 GTGCAC G[AT]GC[AT]^C
640 GAGCTC
AvaII 225 GGACC G^G[AT]CC
456 GGACC
BalI 428 TGGCCA TGG^CCA
464 TGGCCA
BbeI 211 GGCGCC GGCGC^C
256 GGCGCC
BglI 496 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
605 GCCGGACGGGC
BsaHI 211 GGCGCC G[GA]^CG[CT]C
256 GGCGCC
BseRI 112 GAGGAG GAGGAG(10/8)
532 GAGGAG
BsiHKAI 37 GTGCAC G[AT]GC[AT]^C
640 GAGCTC
BstUI 284 CGCG CG^CG
367 CGCG
DraII 262 GGGCCCC [GA]G^G[ACGT]CC[CT]
632 GGGCCCT
EcoO109I 262 GGGCCCC [GA]G^G[ACGT]CC[CT]
632 GGGCCCT
EcoT14I 129 CCATGG C^C[AT][AT]GG
467 CCAAGG
Hin1I 211 GGCGCC G[GA]^CG[CT]C
256 GGCGCC
HinfI 3 GAGTC G^A[ACGT]TC
333 GACTC
KasI 211 GGCGCC G^GCGCC
256 GGCGCC
MluNI 428 TGGCCA TGG^CCA
464 TGGCCA
MscI 428 TGGCCA TGG^CCA
464 TGGCCA
MvnI 284 CGCG CG^CG
367 CGCG
NarI 211 GGCGCC GG^CGCC
256 GGCGCC
PleI 333 GACTC GAGTC(4/5)
3 GAGTC
SfaNI 341 GCATC GCATC(5/9)
374 GCATC
SfoI 211 GGCGCC GGC^GCC
256 GGCGCC
StyI 129 CCATGG C^C[AT][AT]GG
467 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AvaI BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsoBI Bsp1286I BssKI BstNI
Cac8I CfoI Cfr13I EaeI EcoRII
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SfcI TseI