Restriction map for
pF1KB8886
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FbaI
FokI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MboII MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MunI MvaI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 165 GAATTT [GA]^AATT[CT]
AfaI 391 GTAC GT^AC
AlwI 504 GATCC GGATC(4/5)
ApoI 165 GAATTT [GA]^AATT[CT]
BanII 295 GAGCCC G[GA]GC[CT]^C
BbrPI 325 CACGTG CAC^GTG
BbvCI 507 CCTCAGC CCTCAGC(-5/-2)
BbvI 23 GCAGC GCAGC(8/12)
BcnI 33 CCCGG CC^[GC]GG
BmyI 295 GAGCCC G[AGT]GC[ACT]^C
BpmI 181 CTGGAG CTGGAG(16/14)
BsaAI 325 CACGTG [CT]AC^GT[GA]
BseRI 480 CTCCTC GAGGAG(10/8)
Bsp1286I 295 GAGCCC G[AGT]GC[ACT]^C
BssKI 33 CCCGG ^CC[ACGT]GG
DpnII 504 GATC ^GATC
DraII 230 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 35 CGGCCA [CT]^GGCC[GA]
EcoNI 129 CCTCAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 230 AGGTCCT [GA]G^G[ACGT]CC[CT]
HaeIII 36 GGCC GG^CC
HapII 34 CCGG C^CGG
HgaI 386 GCGTC GACGC(5/10)
HpaII 34 CCGG C^CGG
HphI 69 TCACC GGTGA(8/7)
MaeII 326 ACGT A^CGT
MboI 504 GATC ^GATC
MspI 34 CCGG C^CGG
MwoI 14 GCAGTTTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 33 CCCGG CC^[GC]GG
NdeII 504 GATC ^GATC
NlaIII 437 CATG CATG^
PflMI 162 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 325 CACGTG CAC^GTG
PmlI 325 CACGTG CAC^GTG
PpuMI 230 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 391 GTAC GT^AC
Sau3AI 504 GATC ^GATC
ScrFI 33 CCCGG CC^[ACGT]GG
SmlI 266 CTCAAG C^T[CT][GA]AG
SspI 141 AATATT AAT^ATT
TaqI 522 TCGA T^CGA
TseI 23 GCAGC G^C[AT]GC
TthHB8I 522 TCGA T^CGA
Van91I 162 CCAGAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 279 GCGG CCGC(-3/-1)
302 GCGG
AvaII 231 GGTCC G^G[AT]CC
575 GGTCC
BfaI 383 CTAG C^TAG
443 CTAG
BsiYI 129 CCTCAACGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
162 CCAGAATTTGG
BslI 129 CCTCAACGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
162 CCAGAATTTGG
BsmFI 452 GGGAC GGGAC(10/14)
238 GTCCC
BsrI 10 ACTGG ACTGG(1/-1)
101 ACTGG
Cfr13I 231 GGTCC G^G[ACGT]CC
575 GGTCC
HinfI 151 GAATC G^A[ACGT]TC
379 GATTC
MaeI 383 CTAG C^TAG
443 CTAG
NlaIV 294 GGAGCC GG[ACGT]^[ACGT]CC
574 GGGTCC
Sau96I 231 GGTCC G^G[ACGT]CC
575 GGTCC
TfiI 151 GAATC G^A[AT]TC
379 GATTC
TspRI 548 CACTG CA[GC]TG[ACGT][ACGT]^
563 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AluI Cac8I DdeI Fnu4HI ItaI
MnlI MseI Tru9I Tsp509I