Restriction map for
pF1KB8881
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstF5I BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HindIII HpaI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SalI SbfI ScaI SexAI
SfaNI SfcI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI Tru9I TseI TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AcsI 88 GAATTT [GA]^AATT[CT]
AfaI 429 GTAC GT^AC
AlwI 399 GATCC GGATC(4/5)
AlwNI 397 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 266 GGGCCC GGGCC^C
ApoI 88 GAATTT [GA]^AATT[CT]
AvaI 478 CCCGAG C^[CT]CG[GA]G
BbeI 183 GGCGCC GGCGC^C
BbvCI 15 CCTCAGC CCTCAGC(-5/-2)
BglI 155 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 282 GCTGAGC GC^T[ACGT]AGC
BpmI 297 CTCCAG CTGGAG(16/14)
Bpu1102I 282 GCTGAGC GC^T[ACGT]AGC
BsaI 140 GGTCTC GGTCTC(1/5)
BsmAI 141 GTCTC GTCTC(1/5)
BsoBI 478 CCCGAG C^[CT]CG[GA]G
BspMI 231 GCAGGT ACCTGC(4/8)
BsrFI 7 ACCGGC [GA]^CCGG[CT]
BstBI 78 TTCGAA TT^CGAA
BstEII 501 GGTGACC G^GT[ACGT]ACC
BstPI 501 GGTGACC G^GT[ACGT]ACC
BstYI 398 AGATCC [GA]^GATC[CT]
CelII 282 GCTGAGC GC^T[ACGT]AGC
Cfr10I 7 ACCGGC [GA]^CCGG[CT]
DpnII 399 GATC ^GATC
DraII 29 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 156 CCGCGG C^C[GA][CT]GG
EaeI 467 TGGCCG [CT]^GGCC[GA]
EarI 195 GAAGAG CTCTTC(1/4)
EcoNI 33 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 29 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 501 GGTGACC G^GT[ACGT]ACC
HaeII 183 GGCGCC [GA]GCGC^[CT]
HincII 442 GTCAAC GT[CT]^[GA]AC
HindII 442 GTCAAC GT[CT]^[GA]AC
KasI 183 GGCGCC G^GCGCC
Ksp632I 195 GAAGAG CTCTTC(1/4)
KspI 156 CCGCGG CCGC^GG
MaeII 451 ACGT A^CGT
MboI 399 GATC ^GATC
MboII 195 GAAGA GAAGA(8/7)
MflI 398 AGATCC [GA]^GATC[CT]
MslI 273 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 156 CCGCGG C[AC]G^C[GT]G
NarI 183 GGCGCC GG^CGCC
NdeII 399 GATC ^GATC
NlaIII 273 CATG CATG^
NspI 272 GCATGC [GA]CATG^[CT]
NspV 78 TTCGAA TT^CGAA
PleI 302 GACTC GAGTC(4/5)
PpuMI 29 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 429 GTAC GT^AC
SacI 120 GAGCTC GAGCT^C
SacII 156 CCGCGG CCGC^GG
SapI 195 GAAGAGC GCTCTTC(1/4)
Sau3AI 399 GATC ^GATC
SfiI 154 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 183 GGCGCC GGC^GCC
SfuI 78 TTCGAA TT^CGAA
SphI 272 GCATGC GCATG^C
StuI 290 AGGCCT AGG^CCT
TaqI 79 TCGA T^CGA
TfiI 76 GATTC G^A[AT]TC
Tsp509I 89 AATT ^AATT
TthHB8I 79 TCGA T^CGA
XhoII 398 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 183 GGCGCC G[GA]^CG[CT]C
369 GGCGTC
AspHI 108 GAGCAC G[AT]GC[AT]^C
120 GAGCTC
AvaII 30 GGACC G^G[AT]CC
234 GGTCC
BanI 183 GGCGCC G^G[CT][GA]CC
324 GGTGCC
BcnI 438 CCCGG CC^[GC]GG
470 CCGGG
BsaHI 183 GGCGCC G[GA]^CG[CT]C
369 GGCGTC
BseRI 115 GAGGAG GAGGAG(10/8)
174 GAGGAG
BsiHKAI 108 GAGCAC G[AT]GC[AT]^C
120 GAGCTC
BsrBI 207 GAGCGG CCGCTC(-3/-3)
262 GAGCGG
HgaI 370 GCGTC GACGC(5/10)
532 GCGTC
Hin1I 183 GGCGCC G[GA]^CG[CT]C
369 GGCGTC
HinfI 76 GATTC G^A[ACGT]TC
302 GACTC
HphI 501 GGTGA GGTGA(8/7)
435 TCACC
NciI 438 CCCGG CC^[GC]GG
470 CCGGG
SmlI 304 CTCAAG C^T[CT][GA]AG
390 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AccII AciI AluI BanII BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MnlI MspI MvaI MvnI
MwoI NlaIV Sau96I ScrFI Tsp45I