##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsiEI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI Bst1107I
BstBI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KpnI Ksp632I MamI
MboI MfeI MflI MluI MluNI
MscI MunI NaeI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SalI SapI
Sau3AI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 149 TCCGGA T^CCGGA
AcyI 237 GGCGCC G[GA]^CG[CT]C
Alw44I 410 GTGCAC G^TGCAC
AlwNI 86 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 410 GTGCAC G^TGCAC
AspI 311 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 128 TGCGCA TGC^GCA
BbeI 237 GGCGCC GGCGC^C
BbsI 308 GAAGAC GAAGAC(2/6)
BpmI 107 CTGGAG CTGGAG(16/14)
BpuAI 308 GAAGAC GAAGAC(2/6)
BsaHI 237 GGCGCC G[GA]^CG[CT]C
BsaWI 149 TCCGGA [AT]^CCGG[AT]
BseAI 149 TCCGGA T^CCGGA
BseRI 273 GAGGAG GAGGAG(10/8)
BsgI 455 CTGCAC GTGCAG(16/14)
BsmFI 23 GTCCC GGGAC(10/14)
BspEI 149 TCCGGA T^CCGGA
BspHI 318 TCATGA T^CATGA
BspMI 131 GCAGGT ACCTGC(4/8)
BsrI 26 CCAGT ACTGG(1/-1)
BssHII 159 GCGCGC G^CGCGC
BssSI 256 CTCGTG CACGAG(-5/-1)
BstAPI 85 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 141 GGTGACC G^GT[ACGT]ACC
BstF5I 201 CATCC GGATG(2/0)
BstPI 141 GGTGACC G^GT[ACGT]ACC
DdeI 271 CTGAG C^T[ACGT]AG
DraII 21 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 21 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 141 GGTGACC G^GT[ACGT]ACC
FokI 201 CATCC GGATG(9/13)
FspI 128 TGCGCA TGC^GCA
Hin1I 237 GGCGCC G[GA]^CG[CT]C
HinfI 153 GATTC G^A[ACGT]TC
KasI 237 GGCGCC G^GCGCC
KspI 205 CCGCGG CCGC^GG
MaeII 480 ACGT A^CGT
MaeIII 142 GTGAC ^GT[ACGT]AC
MboII 308 GAAGA GAAGA(8/7)
MroI 149 TCCGGA T^CCGGA
MseI 37 TTAA T^TAA
NarI 237 GGCGCC GG^CGCC
PflFI 311 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 31 CTGCAG CTGCA^G
RcaI 318 TCATGA T^CATGA
SacI 97 GAGCTC GAGCT^C
SacII 205 CCGCGG CCGC^GG
SexAI 312 ACCAGGT A^CC[AT]GGT
SfcI 31 CTGCAG C^T[GA][CT]AG
SfoI 237 GGCGCC GGC^GCC
SmaI 423 CCCGGG CCC^GGG
TfiI 153 GATTC G^A[AT]TC
Tru9I 37 TTAA T^TAA
Tsp45I 142 GTGAC ^GT[GC]AC
Tth111I 311 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 423 CCCGGG C^CCGGG
XmaI 423 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 98 AGCT AG^CT
348 AGCT
AspHI 97 GAGCTC G[AT]GC[AT]^C
410 GTGCAC
AvaI 336 CCCGAG C^[CT]CG[GA]G
423 CCCGGG
AvaII 22 GGTCC G^G[AT]CC
511 GGACC
BanI 69 GGTGCC G^G[CT][GA]CC
237 GGCGCC
BanII 97 GAGCTC G[GA]GC[CT]^C
371 GAGCCC
BfaI 286 CTAG C^TAG
305 CTAG
BsiHKAI 97 GAGCTC G[AT]GC[AT]^C
410 GTGCAC
BsoBI 336 CCCGAG C^[CT]CG[GA]G
423 CCCGGG
HgaI 416 GACGC GACGC(5/10)
282 GCGTC
MaeI 286 CTAG C^TAG
305 CTAG
MslI 319 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
442 CACGCTGGTG
NlaIII 123 CATG CATG^
319 CATG
SfaNI 264 GATGC GCATC(5/9)
488 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI BbvI BcnI BglI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DsaI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI