################################## # NO CUT ENZYMES ( 213 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 132 AAATTC [GA]^AATT[CT] AfaI 121 GTAC GT^AC ApoI 132 AAATTC [GA]^AATT[CT] BsiYI 244 CCTACAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 244 CCTACAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 326 GTCTC GTCTC(1/5) BsmI 287 GCATTC GAATGC(1/-1) BsrI 299 CCAGT ACTGG(1/-1) BssKI 160 CCAGG ^CC[ACGT]GG BstNI 160 CCAGG CC^[AT]GG BstXI 6 CCAGCGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 197 CTCAG C^T[ACGT]AG DpnII 174 GATC ^GATC DraI 18 TTTAAA TTT^AAA DsaI 179 CCACGG C^C[GA][CT]GG EarI 85 GAAGAG CTCTTC(1/4) EcoNI 244 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 160 CCAGG ^CC[AT]GG EcoT14I 80 CCTTGG C^C[AT][AT]GG HaeIII 258 GGCC GG^CC HinfI 263 GACTC G^A[ACGT]TC HphI 176 TCACC GGTGA(8/7) Ksp632I 85 GAAGAG CTCTTC(1/4) MboI 174 GATC ^GATC MseI 19 TTAA T^TAA MvaI 160 CCAGG CC^[AT]GG NdeII 174 GATC ^GATC NlaIII 193 CATG CATG^ PleI 263 GACTC GAGTC(4/5) RsaI 121 GTAC GT^AC Sau3AI 174 GATC ^GATC ScrFI 160 CCAGG CC^[ACGT]GG SfcI 245 CTACAG C^T[GA][CT]AG StyI 80 CCTTGG C^C[AT][AT]GG Tru9I 19 TTAA T^TAA Tsp509I 133 AATT ^AATT ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### BfaI 59 CTAG C^TAG 118 CTAG BsaJI 80 CCTTGG C^C[ACGT][ACGT]GG 179 CCACGG BstF5I 73 CATCC GGATG(2/0) 147 GGATG Cac8I 5 GCCAGC GC[ACGT]^[ACGT]GC 239 GCCAGC FokI 73 CATCC GGATG(9/13) 147 GGATG MaeI 59 CTAG C^TAG 118 CTAG MboII 85 GAAGA GAAGA(8/7) 333 TCTTC MnlI 88 GAGG CCTC(7/6) 251 GAGG ######################################### # 3 OR MORE CUT ENZYMES ( 1 enzymes) # ######################################### TspRI