##################################
# NO CUT ENZYMES (  206 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DpnII       DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AcsI          262  GAATTC               [GA]^AATT[CT]
AlwNI         166  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          262  GAATTC               [GA]^AATT[CT]
BbsI          229  GTCTTC               GAAGAC(2/6)
BmyI            6  GTGCCC               G[AGT]GC[ACT]^C
BpmI           13  CTGGAG               CTGGAG(16/14)
BpuAI         229  GTCTTC               GAAGAC(2/6)
BsmFI         197  GGGAC                GGGAC(10/14)
Bsp1286I        6  GTGCCC               G[AGT]GC[ACT]^C
BstF5I         27  GGATG                GGATG(2/0)
BstXI         128  CCAGCTTCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I           4  GCGTGC               GC[ACGT]^[ACGT]GC
Cfr13I         21  GGCCC                G^G[ACGT]CC
DraII          20  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       20  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         262  GAATTC               G^AATTC
FokI           27  GGATG                GGATG(9/13)
HaeIII         21  GGCC                 GG^CC
HapII         117  CCGG                 C^CGG
HpaII         117  CCGG                 C^CGG
HphI           70  GGTGA                GGTGA(8/7)
MslI          243  CATCGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          117  CCGG                 C^CGG
NlaIII        249  CATG                 CATG^
Sau96I         21  GGCCC                G^G[ACGT]CC
SfaNI         242  GCATC                GCATC(5/9)
SmaI          116  CCCGGG               CCC^GGG
Tsp45I         71  GTGAC                ^GT[GC]AC
Tsp509I       263  AATT                 ^AATT
XmaCI         116  CCCGGG               C^CCGGG
XmaI          116  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI           54  GTAC                 GT^AC
              222  GTAC                
AvaI           57  CTCGGG               C^[CT]CG[GA]G
              116  CCCGGG              
BcnI          116  CCCGG                CC^[GC]GG
              117  CCGGG               
BsoBI          57  CTCGGG               C^[CT]CG[GA]G
              116  CCCGGG              
BstNI          24  CCTGG                CC^[AT]GG
              293  CCAGG               
EcoRII         24  CCTGG                ^CC[AT]GG
              293  CCAGG               
MaeIII         71  GTGAC                ^GT[ACGT]AC
              257  GTAAC               
MboII         297  GAAGA                GAAGA(8/7)
              230  TCTTC               
MvaI           24  CCTGG                CC^[AT]GG
              293  CCAGG               
NciI          116  CCCGG                CC^[GC]GG
              117  CCGGG               
RsaI           54  GTAC                 GT^AC
              222  GTAC                
ScaI           53  AGTACT               AGT^ACT
              221  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

AluI        BbvI        BsaJI       BssKI       Fnu4HI      
ItaI        MnlI        ScrFI       TseI