##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        Fnu4HI      
FokI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
TseI        Tsp45I      Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AciI          229  GCGG                 CCGC(-3/-1)
AcsI           52  AAATTC               [GA]^AATT[CT]
AluI          211  AGCT                 AG^CT
ApoI           52  AAATTC               [GA]^AATT[CT]
BsiYI         222  CCTAATTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          222  CCTAATTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrI          180  CCAGT                ACTGG(1/-1)
DdeI           91  CTGAG                C^T[ACGT]AG
DsaI           23  CCATGG               C^C[GA][CT]GG
EcoT14I        23  CCATGG               C^C[AT][AT]GG
FauI          229  GCGGG                CCCGC(4/6)
HaeIII        232  GGCC                 GG^CC
HphI          236  TCACC                GGTGA(8/7)
MseI           76  TTAA                 T^TAA
NcoI           23  CCATGG               C^CATGG
NspI          244  GCATGC               [GA]CATG^[CT]
ScaI           96  AGTACT               AGT^ACT
SphI          244  GCATGC               GCATG^C
StyI           23  CCATGG               C^C[AT][AT]GG
Tru9I          76  TTAA                 T^TAA
TspRI         181  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          180  CCAGTGTAGAGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AfaI           97  GTAC                 GT^AC
              265  GTAC                
BsaJI          23  CCATGG               C^C[ACGT][ACGT]GG
              117  CCCTGG              
BsrDI         240  CATTGC               GCAATG(2/0)
              248  GCAATG              
BssKI         118  CCTGG                ^CC[ACGT]GG
              174  CCTGG               
BstNI         118  CCTGG                CC^[AT]GG
              174  CCTGG               
Cac8I         229  GCGGGC               GC[ACGT]^[ACGT]GC
              244  GCATGC              
DraII          86  AGGACCT              [GA]G^G[ACGT]CC[CT]
              170  AGGACCT             
EcoO109I       86  AGGACCT              [GA]G^G[ACGT]CC[CT]
              170  AGGACCT             
EcoRII        118  CCTGG                ^CC[AT]GG
              174  CCTGG               
MvaI          118  CCTGG                CC^[AT]GG
              174  CCTGG               
MwoI          194  GCAAAGTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              203  GCTTCCAGAGC         
PpuMI          86  AGGACCT              [GA]G^G[AT]CC[CT]
              170  AGGACCT             
RsaI           97  GTAC                 GT^AC
              265  GTAC                
ScrFI         118  CCTGG                CC^[ACGT]GG
              174  CCTGG               
Tsp509I        53  AATT                 ^AATT
              225  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AvaII       Cfr13I      MnlI        NlaIII      Sau96I