Restriction map for
pF1KB8858
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# NO CUT ENZYMES ( 206 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HindIII HinfI HpaI HpaII KasI
KpnI KspI MaeI MaeII MaeIII
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIII NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII Sau3AI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI TfiI
Tru9I Tsp45I TspRI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 205 GTCGAC GT^[AC][GT]AC
AcsI 217 GAATTC [GA]^AATT[CT]
ApoI 217 GAATTC [GA]^AATT[CT]
BpmI 22 CTGGAG CTGGAG(16/14)
BsaBI 184 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 237 CATTGC GCAATG(2/0)
BstF5I 183 GGATG GGATG(2/0)
Cac8I 120 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 241 GCGC GCG^C
EarI 13 GAAGAG CTCTTC(1/4)
EcoNI 81 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 217 GAATTC G^AATTC
EcoT14I 174 CCAAGG C^C[AT][AT]GG
FokI 183 GGATG GGATG(9/13)
HaeIII 178 GGCC GG^CC
HhaI 241 GCGC GCG^C
HinP1I 241 GCGC G^CGC
HincII 205 GTCGAC GT[CT]^[GA]AC
HindII 205 GTCGAC GT[CT]^[GA]AC
HphI 91 GGTGA GGTGA(8/7)
Ksp632I 13 GAAGAG CTCTTC(1/4)
MamI 184 GATGCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 103 CAGCTG C[AC]G^C[GT]G
PflMI 174 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 103 CAGCTG CAG^CTG
SalI 205 GTCGAC G^TCGAC
SapI 13 GAAGAGC GCTCTTC(1/4)
SspI 168 AATATT AAT^ATT
StuI 177 AGGCCT AGG^CCT
StyI 174 CCAAGG C^C[AT][AT]GG
TaqI 206 TCGA T^CGA
TthHB8I 206 TCGA T^CGA
Van91I 174 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 14 enzymes) #
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BsaJI 174 CCAAGG C^C[ACGT][ACGT]GG
227 CCCTGG
BsiYI 81 CCTCTCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
174 CCAAGGCCTGG
BslI 81 CCTCTCTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
174 CCAAGGCCTGG
BssKI 180 CCTGG ^CC[ACGT]GG
228 CCTGG
BstNI 180 CCTGG CC^[AT]GG
228 CCTGG
DdeI 54 CTCAG C^T[ACGT]AG
86 CTAAG
EcoRII 180 CCTGG ^CC[AT]GG
228 CCTGG
MboII 13 GAAGA GAAGA(8/7)
41 TCTTC
MnlI 81 CCTC CCTC(7/6)
25 GAGG
MvaI 180 CCTGG CC^[AT]GG
228 CCTGG
MwoI 96 GCTGAAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
241 GCGCTGAAGGC
ScrFI 180 CCTGG CC^[ACGT]GG
228 CCTGG
SfaNI 184 GATGC GCATC(5/9)
18 GCATC
Tsp509I 30 AATT ^AATT
218 AATT
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# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
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AluI BbvI Fnu4HI ItaI TseI