##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       HphI        ItaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TfiI        
Tru9I       TseI        Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          217  GAATTC               [GA]^AATT[CT]
ApoI          217  GAATTC               [GA]^AATT[CT]
AspHI         103  GAGCTC               G[AT]GC[AT]^C
AspI          163  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         103  GAGCTC               G[GA]GC[CT]^C
BglI           19  GCCATGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          103  GAGCTC               G[AGT]GC[ACT]^C
BpmI           10  CTGGAG               CTGGAG(16/14)
BsiHKAI       103  GAGCTC               G[AT]GC[AT]^C
BsmBI         194  GAGACG               CGTCTC(1/5)
Bsp1286I      103  GAGCTC               G[AGT]GC[ACT]^C
BsrI          180  ACTGG                ACTGG(1/-1)
BssKI         213  CCAGG                ^CC[ACGT]GG
BssSI         256  CACGAG               CACGAG(-5/-1)
BstNI         213  CCAGG                CC^[AT]GG
BstXI          48  CCAATATTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         250  GCCTGC               GC[ACGT]^[ACGT]GC
Cfr13I         27  GGCCC                G^G[ACGT]CC
EcoRI         217  GAATTC               G^AATTC
EcoRII        213  CCAGG                ^CC[AT]GG
MaeII          38  ACGT                 A^CGT
MboII          84  GAAGA                GAAGA(8/7)
MvaI          213  CCAGG                CC^[AT]GG
PflFI         163  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI          103  GAGCTC               GAGCT^C
Sau96I         27  GGCCC                G^G[ACGT]CC
ScrFI         213  CCAGG                CC^[ACGT]GG
SfiI           18  GGCCATGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI           50  AATATT               AAT^ATT
TaqI           35  TCGA                 T^CGA
Tsp45I        206  GTGAC                ^GT[GC]AC
Tsp509I       218  AATT                 ^AATT
TspRI         179  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       163  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        35  TCGA                 T^CGA
XcmI           45  CCACCAATATTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BsaJI          20  CCATGG               C^C[ACGT][ACGT]GG
              236  CCATGG              
BsmAI          68  GAGAC                GTCTC(1/5)
              194  GAGAC               
DdeI            5  CTGAG                C^T[ACGT]AG
              132  CTTAG               
DsaI           20  CCATGG               C^C[GA][CT]GG
              236  CCATGG              
EcoT14I        20  CCATGG               C^C[AT][AT]GG
              236  CCATGG              
MaeIII        206  GTGAC                ^GT[ACGT]AC
              241  GTTAC               
MwoI           19  GCCATGGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              226  GCCTTTGTTGC         
NcoI           20  CCATGG               C^CATGG
              236  CCATGG              
StyI           20  CCATGG               C^C[AT][AT]GG
              236  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AluI        HaeIII      MnlI        NlaIII